In vivo models of mucin biosynthesis and function

Syed, Zulfeqhar A.; Zhang, Liping; Hagen, Karl S.

doi:10.1016/j.addr.2022.114182

Cited by 22 publications

(18 citation statements)

References 122 publications

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“…The in vivo importance of MUC2 was confirmed by the ablation of MUC2 in mice. MUC2 −/− mice displayed aberrant intestinal morphology, increased intestinal permeability and mitochondrial damage, and developed spontaneous colitis and colorectal cancer 43 . These studies highlight the importance of MUC2 in the intestinal barrier.…”

Section: Discussionmentioning

confidence: 71%

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Colonic mucin‐2 attenuates acute necrotizing pancreatitis in rats by modulating intestinal homeostasis

et al. 2023

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Mucin‐2 (MUC2) secreted by goblet cells participates in the intestinal barrier, but its mechanism in acute necrotizing pancreatitis (ANP) remains unclear. In acute pancreatitis (AP) patients, the functions of goblet cells (MUC2, FCGBP, CLCA1, and TFF3) decreased, and MUC2 was negatively correlated with AP severity. ANP rats treated with pilocarpine (PILO) (PILO+ANP rats) to deplete MUC2 showed more serious pancreatic and colonic injuries, goblet cell dysfunction, gut dysbiosis, and bacterial translocation than those of ANP rats. GC‐MS analysis of feces showed that PILO+ANP rats had lower levels of butyric acid, isobutyric acid, isovaleric acid, and hexanoic acid than those of ANP rats. The expression of MUC2 was associated with colonic injury and gut dysbiosis. All these phenomena could be relieved, and goblet cell functions were also partially reversed by MUC2 supplementation in ANP rats. TNF‐α‐treated colonoids had exacerbated goblet cell dysfunction. MUC2 expression was negatively correlated with the levels of pro‐inflammatory cytokines (IL‐1β and IL‐6) (p < .05) and positively related to the expression of tight junction proteins (Claudin 1, Occludin, and ZO1) (p < .05). Downregulating MUC2 by siRNA increased the levels of the pro‐inflammatory cytokines in colonoids. MUC2 might maintain intestinal homeostasis to alleviate ANP.

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Section: Discussionmentioning

confidence: 71%

“…MUC2 −/− mice displayed aberrant intestinal morphology, increased intestinal permeability and mitochondrial damage, and developed spontaneous colitis and colorectal cancer. 43 These studies highlight the importance of MUC2 in the intestinal barrier. Our study concentrated on the function of MUC2 in AP and its potential mechanism.…”

Section: Discussionmentioning

confidence: 95%

Colonic mucin‐2 attenuates acute necrotizing pancreatitis in rats by modulating intestinal homeostasis

et al. 2023

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“…Mucin mRNA levels generally do not re ect mucin synthesis rates because mucins are 85% carbohydrate and technically regulated by post-translational management, such as gltcosylation [58]. Glycosylation increases the number of hydroxyl groups in the intestinal mucosa, which makes it easier to capture exogenous probiotics and make them stay on the intestinal surface [59]. Therefore, RS might increase the number of adhesion receptors in HT-29 cells by promoting the expression of mucin genes and surface glycosylation, making it easier for exogenous probiotics adhere and colonize.…”

Section: Discussionmentioning

confidence: 99%

Effects and Mechanisms of Resveratrol on the Adhesion of Lactobacillus acidophilus NCFM

Yuan

Zhang

Pan

et al. 2022

Probiotics & Antimicro. Prot.

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Based on the adhesion and surface properties of Lactobacillus acidophilus NCFM, ve common polyphenols in fruits and vegetables were screened, and the reasons for resveratrol promoting adhesion were systematically explained. The effects of resveratrol, epicatechin, quercetin, hesperidin and caffeic acid on adhesion and surface properties of NCFM were evaluated. The results showed that resveratrol could signi cantly enhance NCFM adhesion to mucin, followed by epicatechin, caffeic acid and hesperidin, while quercetin had a certain degree of inhibition. The effects of these polyphenols on surface hydrophobicity and auto-aggregation of NCFM were consistent with adhesion results. Then, how resveratrol promotes NCFM adhesion was further explored. The results of proteomic analysis showed that resveratrol changed the surface layer proteins of NCFM, involving changes in the relative abundance of 16 proteins. In addition, resveratrol promoted the expression of mucin genes and the glycosylation mucins on the HT-29 cell surface. Our results indicate that resveratrol changes the surface layer proteins of NCFM to modify surface properties and adhere to mucins. Meanwhile resveratrol promotes expression and glycosylation of mucins in HT-29 cells. Our ndings provide theoretical support for an in-depth explanation of the interaction among resveratrol, NCFM and the HT-29 cells.

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“…Once the secretion begins, the paracrystalline structure is lost and the lumen is filled with an amorph secretion [ 37 , 38 ]. A rise in pH and disappearance of calcium ions lead to the unfolding and hydration of the granule contents with water coming from the hemolymph, increasing the total volume [ 39 , 40 ]. At the end of the third instar larval stage, the salivary gland becomes bloated and full of glue.…”

Section: Production and Expectoration Of The Gluementioning

confidence: 99%

“…The glue may also act as a preservative and avoid fungal or bacterial infections. The glycoproteins that make up the glue belong to the mucin family, and mucins are known to have antimicrobial properties [ 39 ]. In particular, the glue protein Eig71Ee, also named gp150, which is present in hemolymph, lies in structures that entrap bacteria [ 53 ].…”

Section: Role Of Drosophila Glue In Natural Environmentsmentioning

confidence: 99%

Drosophila Glue: A Promising Model for Bioadhesion

Monier

Courtier‐Orgogozo

2022

Insects

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The glue produced by Drosophila larvae to attach themselves to a substrate for several days and resist predation until the end of metamorphosis represents an attractive model to develop new adhesives for dry environments. The adhesive properties of this interesting material have been investigated recently, and it was found that it binds as well as strongly adhesive commercial tapes to various types of substrates. This glue hardens rapidly after excretion and is made of several proteins. In D. melanogaster, eight glue proteins have been identified: four are long glycosylated mucoproteins containing repeats rich in prolines, serines and threonines, and four others are shorter proteins rich in cysteines. This protein mix is produced by the salivary glands through a complex packaging process that is starting to be elucidated. Drosophila species have adapted to stick to various substrates in diverse environmental conditions and glue genes appear to evolve rapidly in terms of gene number, number of repeats and sequence of the repeat motifs. Interestingly, besides its adhesive properties, the glue may also have antimicrobial activities. We discuss future perspectives and avenues of research for the development of new bioadhesives mimicking Drosophila fly glue.

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In vivo models of mucin biosynthesis and function

Cited by 22 publications

References 122 publications

Colonic mucin‐2 attenuates acute necrotizing pancreatitis in rats by modulating intestinal homeostasis

Colonic mucin‐2 attenuates acute necrotizing pancreatitis in rats by modulating intestinal homeostasis

Effects and Mechanisms of Resveratrol on the Adhesion of Lactobacillus acidophilus NCFM

Drosophila Glue: A Promising Model for Bioadhesion

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