In vivo model of Propionibacterium (Cutibacterium) spp. biofilm in Drosophila melanogaster

Bronnec, Vicky; Alexeyev, Oleg A.

doi:10.1016/j.anaerobe.2021.102450

Cited by 5 publications

(10 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently a germ-free Drosophila melanogaster (fruit fly) model was developed to study C. acnes , C. avidum and Cutibacterium granulosum biofilms [ 144 ]. By maintaining the fruit flies on a lipid-rich diet, an anaerobic lipid-rich environment is created in the gut, which mimics the environment of the hair follicle.…”

Section: Model Systems To Study C Acnes Biofilm Formationmentioning

confidence: 99%

The role of biofilm formation in the pathogenesis and antimicrobial susceptibility of Cutibacterium acnes

Coenye

Spittaels

Achermann

2022

Biofilm

View full text Add to dashboard Cite

Cutibacterium acnes (previously known as Propionibacterium acnes ) is frequently found on lipid-rich parts of the human skin. While C. acnes is most known for its role in the development and progression of the skin disease acne, it is also involved in many other types of infections, often involving implanted medical devices. C. acnes readily forms biofilms in vitro and there is growing evidence that biofilm formation by this Gram-positive, facultative anaerobic micro-organism plays an important role in vivo and is also involved in treatment failure. In this brief review we present an overview on what is known about C. acnes biofilms (including their role in pathogenesis and reduced susceptibility to antibiotics), discuss model systems that can be used to study these biofilms in vitro and in vivo and give an overview of interspecies interactions occurring in polymicrobial communities containing C. acnes .

show abstract

Section: Model Systems To Study C Acnes Biofilm Formationmentioning

confidence: 99%

The role of biofilm formation in the pathogenesis and antimicrobial susceptibility of Cutibacterium acnes

Coenye

Spittaels

Achermann

2022

Biofilm

View full text Add to dashboard Cite

show abstract

“…Bacterial Strains and Planktonic Culture Conditions P. acnes KPA171202 and P. granulosum DSM 20700 (DSMZ, Braunschweig, Germany) were cultured under anaerobic conditions at 37°C and rotation 250 rpm as previously described (Bronnec and Alexeyev, 2021).…”

Section: Methodsmentioning

confidence: 99%

“…Total DNA from P. granulosum was extracted as previously described (Bronnec and Alexeyev, 2021). Paired-end sequencing was performed using Illumina HiSeq2000 2x100 bp runs.…”

Section: P Granulosum Dsm 20700 Genome Sequencingmentioning

confidence: 99%

“…Several volumes (1 µL, 3 µL and 5 µL) of this faction were incubated with 500 ng of P. acnes DNA for two hours at 37°C. P. acnes DNA was extracted as previously described (Bronnec and Alexeyev, 2021). As controls, reaction mix were supplemented with 3 mM MgCl 2 and 10 mM EDTA.…”

Section: Dnase Activity Assaysmentioning

confidence: 99%

“…The in vivo model of P. acnes biofilm in Drosophila melanogaster (Bronnec and Alexeyev, 2021) has been used to evaluate the dispersal activity of BmdE. Briefly, the feeding infection method has been used to develop a mature biofilm of acnes in the fruit fly gut.…”

Section: In Vivo P Acnes Biofilm Dispersion With R-bmdementioning

confidence: 99%

See 2 more Smart Citations

Propionibacterium (Cutibacterium) granulosum Extracellular DNase BmdE Targeting Propionibacterium (Cutibacterium) acnes Biofilm Matrix, a Novel Inter-Species Competition Mechanism

Bronnec

Eilers

Jahns

et al. 2022

Front. Cell. Infect. Microbiol.

Self Cite

View full text Add to dashboard Cite

Acne vulgaris is the most common dermatological disorder worldwide affecting more than 80% of adolescents and young adults with a global prevalence of 231 million cases in 2019. The involvement of the skin microbiome disbalance in the pathophysiology of acne is recognized, especially regarding the relative abundance and diversity of Propionibacterium acnes a well-known dominant human skin commensal. Biofilms, where bacteria are embedded into a protective polymeric extracellular matrix, are the most prevalent life style for microorganisms. P. acnes and its biofilm-forming ability is believed to be a contributing factor in the development of acne vulgaris, the persistence of the opportunistic pathogen and antibiotic therapy failures. Degradation of the extracellular matrix is one of the strategies used by bacteria to disperse the biofilm of competitors. In this study, we report the identification of an endogenous extracellular nuclease, BmdE, secreted by Propionibacterium granulosum able to degrade P. acnes biofilm both in vivo and in vitro. This, to our knowledge, may represent a novel competitive mechanism between two closely related species in the skin. Antibiotics targeting P. acnes have been the mainstay in acne treatment. Extensive and long-term use of antibiotics has led to the selection and spread of resistant bacteria. The extracellular DNase BmdE may represent a new bio-therapeutical strategy to combat P. acnes biofilm in acne vulgaris.

show abstract

Detailed protocol for germ-free Drosophila melanogaster colonization with Propionibacterium spp. biofilms

Bronnec

Alexeyev

2022

STAR Protocols

Self Cite

View full text Add to dashboard Cite

In vivo model of Propionibacterium (Cutibacterium) spp. biofilm in Drosophila melanogaster

Cited by 5 publications

References 49 publications

The role of biofilm formation in the pathogenesis and antimicrobial susceptibility of Cutibacterium acnes

The role of biofilm formation in the pathogenesis and antimicrobial susceptibility of Cutibacterium acnes

Propionibacterium (Cutibacterium) granulosum Extracellular DNase BmdE Targeting Propionibacterium (Cutibacterium) acnes Biofilm Matrix, a Novel Inter-Species Competition Mechanism

Detailed protocol for germ-free Drosophila melanogaster colonization with Propionibacterium spp. biofilms

Contact Info

Product

Resources

About