In Vivo Microelectrode Arrays for Detecting Multi-Region Epileptic Activities in the Hippocampus in the Latent Period of Rat Model of Temporal Lobe Epilepsy

Dai, Yuchuan; Song, Yilin; Xie, Jingyu; Xu, Shengwei; Li, Xinrong; He, Enhui; Yin, Huabing; Cai, Xinxia

doi:10.3390/mi12060659

Cited by 7 publications

(6 citation statements)

References 30 publications

(31 reference statements)

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“…For improved visualization of the frequency distribution, we linearly interpolated the initial 32 by 4 sites to generate an 875 by 656 pixel plot, which was subsequently overlaid with the mouse brain atlas as per the method outlined. [ 48 ] Readers can find the original, non‐interpolated plot in the supplementary materials. This visualization reveals a ripple pattern emerging at coordinates AP −1.5, ML −1.95 mm, DV 1.4 mm.…”

Section: Resultsmentioning

confidence: 99%

High‐Resolution Recording of Neural Activity in Epilepsy Using Flexible Neural Probes

Cheng,

Li,

Tian

et al. 2023

Adv Materials Technologies

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Epilepsy, a prevalent neurological disorder, necessitates precise and reliable electrophysiological recording for accurate study and diagnosis. Although traditional stereo‐electroencephalography and micro‐wire probes are widely used in epilepsy research, they are limited by recording site numbers and mechanical properties. This study explores the use of high‐density, ultra‐flexible neural probes in epilepsy monitoring, highlighting their advantages in terms of recording performance, scalability, and tissue compatibility. This work validates the effectiveness of the probes in detecting characteristic epileptic signals across various stages, including resting, preictal, and ictal stages, in two different mouse models of epilepsy. Additionally, the high spatial resolution of the probes allows to capture fine spatial propagation of epilepsy‐associated high‐frequency oscillations. Furthermore, the flexible probes exhibit superior biocompatibility, reducing inflammation and neuronal apoptosis compared to rigid electrodes. The results underscore the promising potential of ultra‐flexible neural probes for advancing epilepsy research, providing a powerful technological platform for future studies in the field.

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Section: Resultsmentioning

confidence: 99%

High‐Resolution Recording of Neural Activity in Epilepsy Using Flexible Neural Probes

Cheng,

Li,

Tian

et al. 2023

Adv Materials Technologies

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“…Invasive methods include intracranial EEG (iEEG) using depth or subdural EEG recordings (ECoG) to study seizure onset and spread as well as SD and seizure propagation in SUDEP models at a higher spatiotemporal resolution (22,30). Stereotaxically inserted multi-channel electrodes can record local field potentials (LFPs) and single-cell activity (204,205). Modulation of neuronal activity via electrical stimulation with these electrodes is possible but is much less precise than optogenetic manipulation.…”

Section: Techniques To Study Network Interaction Involved In Sudepmentioning

confidence: 99%

Autonomic dysfunction in epilepsy mouse models with implications for SUDEP research

et al. 2023

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Epilepsy has a high prevalence and can severely impair quality of life and increase the risk of premature death. Sudden unexpected death in epilepsy (SUDEP) is the leading cause of death in drug-resistant epilepsy and most often results from respiratory and cardiac impairments due to brainstem dysfunction. Epileptic activity can spread widely, influencing neuronal activity in regions outside the epileptic network. The brainstem controls cardiorespiratory activity and arousal and reciprocally connects to cortical, diencephalic, and spinal cord areas. Epileptic activity can propagate trans-synaptically or via spreading depression (SD) to alter brainstem functions and cause cardiorespiratory dysfunction. The mechanisms by which seizures propagate to or otherwise impair brainstem function and trigger the cascading effects that cause SUDEP are poorly understood. We review insights from mouse models combined with new techniques to understand the pathophysiology of epilepsy and SUDEP. These techniques include in vivo, ex vivo, invasive and non-invasive methods in anesthetized and awake mice. Optogenetics combined with electrophysiological and optical manipulation and recording methods offer unique opportunities to study neuronal mechanisms under normal conditions, during and after non-fatal seizures, and in SUDEP. These combined approaches can advance our understanding of brainstem pathophysiology associated with seizures and SUDEP and may suggest strategies to prevent SUDEP.

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“…Temporal lobe epilepsy (TLE) is refractory focal epilepsy, [25] characterized by synchronous or abnormal discharge of brain neurons, which often originates in the CA3 region of the hippocampus [26,27]. Through in vitro and in vivo animal epilepsy models, it was determined that temporal lobe epilepsy begins in the CA3 area of the hippocampal horn, and the generated electrophysiological signals are gradually transmitted to the DG and CA1 areas to form local abnormal discharges [26,27]. At the same time, they are transmitted to the amygdala on the same side, even to the entire limbic system and the contralateral brain area, ultimately forming a group of epileptic discharges.…”

Section: Introductionmentioning

confidence: 99%

AsHC 360 Exposure Influence on Epileptiform Discharges in Hippocampus of Infantile Male Rats In Vitro

Dong,

Zhao,

Tian

et al. 2023

IJMS

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Arsenic-containing hydrocarbons (AsHCs) are typical arsenolipids found in various marine organisms. They can penetrate the blood–brain barrier, specifically affecting synaptic plasticity and the learning and memory ability of hippocampal neurons. Temporal lobe epilepsy often occurs in the hippocampus. Thus, the possible influence of AsHCs exposure to temporal lobe epilepsy garnered attention. The present study investigated the effects of epileptiform discharges (EDs) signals introduced by low-magnesium ACSF in the hippocampus of infantile male rats in vitro, using electrophysiological techniques with multi-electrode arrays under AsHC 360 exposure. In our study of the effects of AsHC 360 on EDs signals, we found that inter-ictal discharges (IIDs) were not significantly impacted. When AsHC 360 was removed, any minor effects observed were reversed. However, when we examined the impact of AsHC 360 on ictal discharges (IDs), distinct patterns emerged based on the concentration levels. For low-concentration groups (5, 20, 60 μg As L−1), both the frequency and duration effects on IDs returned to normal post-elimination of AsHC 360. However, this recovery was not evident for concentrations of 100 μg As L−1 or higher. IDs were only observed in EDs signals during exposures to AsHC 360 concentrations up to 60 μg As L−1. In these conditions, ID frequencies significantly enhanced with the increased of AsHC 360 concentration. At high concentrations of AsHC 360 (≥100 μg As L−1), the transition from IIDs or pre-ictal discharges (PIDs) to IDs was notably inhibited. Additional study on co-exposure of AsHC 360 (100 μg As L−1) and agonist (10 nM (S)-(-)-Bay-K-8644) indicated that the regulation of EDs signals under AsHC 360 exposure could be due to directly interference with the α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor (AMPAR) expression which influences the binding of excitatory glutamate neurotransmitter to AMPAR. The results suggest that EDs activities in the hippocampus of infantile Sprague Dawley rats are concentration-dependent on AsHC 360 exposure. Thus, it provides a basis for the seafood intake with AsHCs for epileptic patients and those with potential seizures.

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In Vivo Microelectrode Arrays for Detecting Multi-Region Epileptic Activities in the Hippocampus in the Latent Period of Rat Model of Temporal Lobe Epilepsy

Cited by 7 publications

References 30 publications

High‐Resolution Recording of Neural Activity in Epilepsy Using Flexible Neural Probes

High‐Resolution Recording of Neural Activity in Epilepsy Using Flexible Neural Probes

Autonomic dysfunction in epilepsy mouse models with implications for SUDEP research

AsHC 360 Exposure Influence on Epileptiform Discharges in Hippocampus of Infantile Male Rats In Vitro

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