In Vivo Microdialysis: A Method for Sampling Extracellular Fluid in Discrete Brain Regions

Krebs-Kraft, Desiree L.; Frantz, Kyle J.; Parent, Marise B.

doi:10.1007/978-0-387-30401-4_9

Cited by 9 publications

(7 citation statements)

References 345 publications

(428 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cages were situated in a temperature and humidity controlled room on the same light/dark schedule used in the vivarium. Rats were given approximately 24 h to recover from the surgery prior to the collection of microdialysis samples, which is accepted as a sufficient period of time for brain tissue to normalize after probe implantation (Krebs-Kraft et al 2007). Rats had ad libitum access to food and water during recovery, but not during the experiment.…”

Section: Methodsmentioning

confidence: 99%

Impact of metabotropic glutamate 2/3 receptor stimulation on activated dopamine release and locomotion

Pehrson

Moghaddam

2010

Psychopharmacology

View full text Add to dashboard Cite

Rationale-Activation of metabotropic glutamate (mGlu) 2/3 receptors may provide a novel strategy for treating schizophrenia. This effect is thought to be mediated through dopamineindependent mechanisms because mGlu2/3-receptor agonists have no considerable affinity for dopamine receptors. These agonists, however, reduce amphetamine-induced hyperlocomotion suggesting that they influence dopamine neurotransmission.Objective-We evaluated whether the inhibitory effect of mGlu2/3-receptor activation on amphetamine-induced hyper-locomotion correlates with attenuated dopamine release. We also assessed whether mGlu 2/3 receptor activation has inhibitory effects on activity-dependent vesicular release of dopamine in behaving animals.Methods-Microdialysis was used to measure extracellular levels of dopamine in the dorsal striatum (DStr) and nucleus accumbens (NAc) of freely moving rats. The effect of the mGlu2/3-receptor agonist LY354740 on dopamine release and locomotion elicited by amphetamine, electrical stimulation of the ventral tegmental area, or L-dopa was assessed.Results-We find that the inhibitory effect of mGlu2/3 activation on amphetamine-induced hyperlocomotion correlates with an attenuated increase in dopamine release in the NAc and DStr. However, when dopamine levels were increased by electrical stimulation of dopamine neurons or by administration of the dopamine precursor L-dopa, activation of mGlu2/3 receptors had no effect on dopamine release or on behavior.Conclusions-Activation of mGlu2/3 receptors attenuates amphetamine-induced dopamine release through a mechanism that does not affect activity dependent vesicular release, reuptake or synthesis of dopamine.

show abstract

Section: Methodsmentioning

confidence: 99%

Impact of metabotropic glutamate 2/3 receptor stimulation on activated dopamine release and locomotion

Pehrson

Moghaddam

2010

Psychopharmacology

View full text Add to dashboard Cite

show abstract

“…The most widely adopted in vivo sampling technique for tonic DA detection is microdialysis [ 44 , 45 , 46 ], which must be coupled with an analytical method such as high-performance liquid chromatography (HPLC) or electrochemical techniques [ 47 , 48 , 49 ], to identify and quantify the neurochemicals in the dialysate. Additionally, it suffers from poor temporal resolution (minutes [ 50 ]), preventing direct correlation of tonic and phasic DA concentrations to neural spiking activity [ 51 , 52 ].…”

Section: Introductionmentioning

confidence: 99%

Flexible Glassy Carbon Multielectrode Array for In Vivo Multisite Detection of Tonic and Phasic Dopamine Concentrations

Castagnola

Robbins

et al. 2022

Biosensors

View full text Add to dashboard Cite

Dopamine (DA) plays a central role in the modulation of various physiological brain functions, including learning, motivation, reward, and movement control. The DA dynamic occurs over multiple timescales, including fast phasic release, as a result of neuronal firing and slow tonic release, which regulates the phasic firing. Real-time measurements of tonic and phasic DA concentrations in the living brain can shed light on the mechanism of DA dynamics underlying behavioral and psychiatric disorders and on the action of pharmacological treatments targeting DA. Current state-of-the-art in vivo DA detection technologies are limited in either spatial or temporal resolution, channel count, longitudinal stability, and ability to measure both phasic and tonic dynamics. We present here an implantable glassy carbon (GC) multielectrode array on a SU-8 flexible substrate for integrated multichannel phasic and tonic measurements of DA concentrations. The GC MEA demonstrated in vivo multichannel fast-scan cyclic voltammetry (FSCV) detection of electrically stimulated phasic DA release simultaneously at different locations of the mouse dorsal striatum. Tonic DA measurement was enabled by coating GC electrodes with poly(3,4-ethylenedioxythiophene)/carbon nanotube (PEDOT/CNT) and using optimized square-wave voltammetry (SWV). Implanted PEDOT/CNT-coated MEAs achieved stable detection of tonic DA concentrations for up to 3 weeks in the mouse dorsal striatum. This is the first demonstration of implantable flexible MEA capable of multisite electrochemical sensing of both tonic and phasic DA dynamics in vivo with chronic stability.

show abstract

“…Furthermore, microdialysis experiments are generally performed in a limited timeframe. In most studies published, experiments are initiated 16 -24 h after probe implantation with a maximal duration of 48 -72 h based on BBB functionality and formation of glial scar tissue [24][25][26][27][28][29][30][31]. However, there appears to be a lack of consensus relating to the integrity status of the BBB which is especially an important aspect when studying BBB transport [28,[31][32][33][34][35].…”

Section: Introductionmentioning

confidence: 99%

Applicability of cerebral open flow microperfusion and microdialysis to quantify a brain-penetrating nanobody in mice

Custers

Wouters

Jaspers

et al. 2021

Analytica Chimica Acta

View full text Add to dashboard Cite

In Vivo Microdialysis: A Method for Sampling Extracellular Fluid in Discrete Brain Regions

Cited by 9 publications

References 345 publications

Impact of metabotropic glutamate 2/3 receptor stimulation on activated dopamine release and locomotion

Impact of metabotropic glutamate 2/3 receptor stimulation on activated dopamine release and locomotion

Flexible Glassy Carbon Multielectrode Array for In Vivo Multisite Detection of Tonic and Phasic Dopamine Concentrations

Applicability of cerebral open flow microperfusion and microdialysis to quantify a brain-penetrating nanobody in mice

Contact Info

Product

Resources

About