In vivo microbiome and associated immune markers: New insights into the pathogenesis of vaginal dysbiosis

Campisciano, Giuseppina; Zanotta, Nunzia; Licastro, Danilo; Seta, Francesco De; Comar, Manola

doi:10.1038/s41598-018-20649-x

Cited by 52 publications

(46 citation statements)

References 44 publications

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“…Taken together, we cannot exclude for a possible contribution of these core gut microbiota in promoting advanced PCa presentation within Africa. Associations to consider include, Bacteroides abundance within vaginal communities of women with bacterial vaginosis (BV), a common condition impacting southern African women, and Bacteroides abundance within the gut mucosa of patients with colorectal cancer …”

Section: Discussionmentioning

confidence: 99%

Metagenomic analysis reveals a rich bacterial content in high‐risk prostate tumors from African men

et al. 2019

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Background Inflammation is a hallmark of prostate cancer (PCa), yet no pathogenic agent has been identified. Men from Africa are at increased risk for both aggressive prostate disease and infection. We hypothesize that pathogenic microbes may be contributing, at least in part, to high‐risk PCa presentation within Africa and in turn the observed ethnic disparity. Methods Here we reveal through metagenomic analysis of host‐derived whole‐genome sequencing data, the microbial content within prostate tumor tissue from 22 men. What is unique about this study is that patients were separated by ethnicity, African vs European, and environments, Africa vs Australia. Results We identified 23 common bacterial genera between the African, Australian, and Chinese prostate tumor samples, while nonbacterial microbes were notably absent. While the most abundant genera across all samples included: Escherichia, Propionibacterium, and Pseudomonas, the core prostate tumor microbiota was enriched for Proteobacteria. We observed a significant increase in the richness of the bacterial communities within the African vs Australian samples (t = 4.6‐5.5; P = .0004‐.001), largely driven by eight predominant genera. Considering core human gut microbiota, African prostate tissue samples appear enriched for Escherichia and Acidovorax, with an abundance of Eubacterium associated with host tumor hypermutation. Conclusions Our study provides suggestive evidence for the presence of a core, bacteria‐rich, prostate microbiome. While unable to exclude for fecal contamination, the observed increased bacterial content and richness within the African vs non‐African samples, together with elevated tumor mutational burden, suggests the possibility that bacterially‐driven oncogenic transformation within the prostate microenvironment may be contributing to aggressive disease presentation in Africa.

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Section: Discussionmentioning

confidence: 99%

Metagenomic analysis reveals a rich bacterial content in high‐risk prostate tumors from African men

et al. 2019

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“…Immediately before the oocyte retrieval, the vagina was irrigated with 50 mL of sterile water, and 3 mL of cervico‐vaginal lavage fluid was collected for our investigations. Cytokine analysis was performed using magnetic bead‐based multiplex immunoassays (Bio‐Plex®; BIO‐RAD Laboratories, Milano, Italy) using the Bio‐Plex 200 reader (Luminex, Austin, TX, USA), as previously described . In brief, 50 µL of cervico‐vaginal washing and standards was added in duplicate to a 96‐multiwell plate containing analyte beads.…”

Section: Methodsmentioning

confidence: 99%

Cervico‐vaginal secretion cytokine profile: A non‐invasive approach to study the endometrial receptivity in IVF cycles

Zanotta

Monasta

Skerk

et al. 2018

American J Rep Immunol

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Several assisted reproductive techniques (ART), including conventional in vitro fertilization (IVF) or intra-cytoplasmic sperm injection (ICSI), are currently available for treatment of infertility. In most ART treatment cycles, one or more good-quality embryos are obtained.Nevertheless, in the last world report, the mean delivery rate per aspiration for fresh cycles reached approximately the 20%, 1 suggesting that a large percentage of good-quality embryos fail to implant.Successful embryo implantation and pregnancy require not only a good-quality embryo, but also a receptive endometrium and effective endometrial-embryonic interactions. 2 This embryo-uterine cross-talk seems to be regulated by several molecules leading to modulation of different cellular functions, such as maternal steroid Problem: Cytokines have a significant role in the process of embryo implantation, trophoblast growth, and differentiation by modulating the immune and endocrine system. The aim of this study was to investigate the profile of a large set of cytokines in the cervico-vaginal washing of women undergoing IVF, to explore the association of these proteins with a good receptive endometrium. Method of study:A cohort of 155 women scheduled for IVF cycle was recruited. All patients were asymptomatic for genitourinary infections and had been screened for chlamydia, mycoplasma, and other bacterial infections. All IVF subjects were treated according to standard clinical and laboratory protocols. A panel of 48 immune factors was analyzed on cervico-vaginal washing, using magnetic bead-based multiplex immunoassays (Bio-Plex, BIO-RAD Laboratories, Milano, Italy). Results:A total of 99 patients reached embryo transfer, of which 31 had a clinical pregnancy. A pattern of four pro-inflammatory immune molecules, IL-12p40, IFN-a, MIF, and MCP3 (P < 0.001), was found significantly up-regulated in the cervico-vaginal fluid of women with clinical pregnancy. A significantly increased expression of IL-9, Gro α , and SDF-1 α (P < 0.05) was observed in the presence of endometriosis, while high levels of IL-13 and L-15 were associated with ovulatory infertility factor (P < 0.05). Conclusion:In this pilot study, we demonstrated that the expression of specific cytokines in the cervico-vaginal washing on the day of oocyte retrieval might have a positive correlation with the potential clinical pregnancy. Therefore, cervico-vaginal secretion cytokine profiling might be a new, non-invasive approach to study the endometrial receptivity in IVF management. K E Y W O R D S cytokines, endometrium, implantation, infertility, IVF, pregnancy How to cite this article: Zanotta N, Monasta L, Skerk K, et al. Cervico-vaginal secretion cytokine profile: A non-invasive approach to study the endometrial receptivity in IVF cycles. Am J Reprod Immunol. 2019;81:e13064. https://doi.

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“…These results suggest that there are significant differences in vaginal microbiota related to ancestry (53); such differences might explain the observed prevalence of bacterial vaginosis and preterm birth. Vaginal dysbiosis is associated with increased levels of proinflammatory cytokines (58). Recently, Fettweis et al (53) observed that levels of the vaginal inflammatory cytokine CXCL10 were related to the L. crispatus/L.…”

Section: The Vaginal Microbiotamentioning

confidence: 99%

Recent Insights on the Maternal Microbiota: Impact on Pregnancy Outcomes

et al. 2020

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In vivo microbiome and associated immune markers: New insights into the pathogenesis of vaginal dysbiosis

Cited by 52 publications

References 44 publications

Metagenomic analysis reveals a rich bacterial content in high‐risk prostate tumors from African men

Metagenomic analysis reveals a rich bacterial content in high‐risk prostate tumors from African men

Cervico‐vaginal secretion cytokine profile: A non‐invasive approach to study the endometrial receptivity in IVF cycles

Recent Insights on the Maternal Microbiota: Impact on Pregnancy Outcomes

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