In vivo Microbial Diversity Analysis on Different Surfaces of Dental Restorative Materials via 16S rDNA Sequencing

Tu, Youying; Wang, Yuan; Su, Lei; Shao, Beibei; Duan, Zhuhui; Deng, Shengjue

doi:10.12659/msm.923509

Cited by 3 publications

(4 citation statements)

References 45 publications

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“…In situ studies involve patients wearing dental appliances carrying specimens that are exposed to natural saliva and biofilms. The specimens are removed periodically and evaluated with objective and sophisticated laboratory methods such as transmission electron microscopy (TEM) to study nanoleakage (Hass et al 2016) and advanced genomic sequencing to study the microbiome related to interfacial degradation (Tu et al 2020). Hence, in situ studies are considered a bridge between clinical studies, which are limited in providing mechanistic understandings of degradation, and in vitro studies, which are limited in simulating intraoral conditions.…”

Section: Clinical Modelsmentioning

confidence: 99%

“…In situ studies conducted on the tooth–composite interface have mainly investigated 1) the effect of the physical and chemical properties of restorative materials on the biofilm composition and vice versa (Tu et al 2020) and 2) the effectiveness of additives in inhibiting degradation of restorative materials, particularly the hybrid layer (Hass et al 2016; Melo et al 2018).…”

Section: Clinical Modelsmentioning

confidence: 99%

“…In addition, in situ studies can be constrained by study duration, subject variability, and noncompliance. Reported study durations ranged between 48 h (Tu et al 2020) and 30 d (Kusuma Yulianto et al 2019), and questions remain as to whether this is sufficient to study the degradative effect of biofilms. While study duration can potentially be extended, ethical and compliance concerns of extended appliance use are substantial barriers.…”

Section: Clinical Modelsmentioning

confidence: 99%

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A Review of Mechano-Biochemical Models for Testing Composite Restorations

Ning

Aregawi

et al. 2021

J Dent Res

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Due to the severe mechano-biochemical conditions in the oral cavity, many dental restorations will degrade and eventually fail. For teeth restored with resin composite, the major modes of failure are secondary caries and fracture of the tooth or restoration. While clinical studies can answer some of the more practical questions, such as the rate of failure, fundamental understanding on the failure mechanism can be obtained from laboratory studies using simplified models more effectively. Reviewed in this article are the 4 main types of models used to study the degradation of resin–composite restorations, namely, animal, human in vivo or in situ, in vitro biofilm, and in vitro chemical models. The characteristics, advantages, and disadvantages of these models are discussed and compared. The tooth–restoration interface is widely considered the weakest link in a resin composite restoration. To account for the different types of degradation that can occur (i.e., demineralization, resin hydrolysis, and collagen degradation), enzymes such as esterase and collagenase found in the oral environment are used, in addition to acids, to form biochemical models to test resin–composite restorations in conjunction with mechanical loading. Furthermore, laboratory tests are usually performed in an accelerated manner to save time. It is argued that, for an accelerated multicomponent model to be representative and predictive in terms of both the mode and the speed of degradation, the individual components must be synchronized in their rates of action and be calibrated with clinical data. The process of calibrating the in vitro models against clinical data is briefly described. To achieve representative and predictive in vitro models, more comparative studies of in vivo and in vitro models are required to calibrate the laboratory studies.

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Section: Clinical Modelsmentioning

confidence: 99%

Section: Clinical Modelsmentioning

confidence: 99%

Section: Clinical Modelsmentioning

confidence: 99%

See 1 more Smart Citation

A Review of Mechano-Biochemical Models for Testing Composite Restorations

Ning

Aregawi

et al. 2021

J Dent Res

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“…We also asked some volunteers to wear a plaque-collection device equipped with four restorative material sheets for two days, collected the plaque samples, and applied Miseq sequencing to obtain template DNA fragments for microbial diversity analysis. The results showed that the types and abundance of bacterial species were significantly influenced by the physicochemical properties of restorative materials [ 14 ] ( Table 1 ). In addition, other factors, such as diabetes, smoking, AIDS, etc., can change the local host environment and promote biofilm formation, leading to various oral diseases [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Interaction between microorganisms and dental material surfaces: general concepts and research progress

Ren

et al. 2023

Journal of Oral Microbiology

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Bacterial adhesion to dental materials’ surfaces is the initial cause of dental materials-related infections. Therefore, inhibiting bacterial adhesion is a critical step in preventing and controlling these infections. To this end, it is important to know how the properties of dental materials affect the interactions between microorganisms and material surfaces to produce materials without biological contamination. This manuscript reviews the mechanism of bacterial adhesion to dental materials, the relationships between their surface properties and bacterial adhesion, and the impact of bacterial adhesion on their surface properties. In addition, this paper summarizes how these surface properties impact oral biofilm formation and proposes designing intelligent dental material surfaces that can reduce biological contamination.

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Evaluation of 12-hour in situ bacterial colonization on smooth restorative material surfaces

Burrow

Leung

2022

Journal of Dentistry

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In vivo Microbial Diversity Analysis on Different Surfaces of Dental Restorative Materials via 16S rDNA Sequencing

Cited by 3 publications

References 45 publications

A Review of Mechano-Biochemical Models for Testing Composite Restorations

A Review of Mechano-Biochemical Models for Testing Composite Restorations

Interaction between microorganisms and dental material surfaces: general concepts and research progress

Evaluation of 12-hour in situ bacterial colonization on smooth restorative material surfaces

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