In vivo micro particle image velocimetry measurements of blood-plasma in the embryonic avian heart

Vennemann, Peter; Kiger, Ken; Lindken, Ralph; Groenendijk, Bianca C.W.; Vos, Sandra Stekelenburg-de; Hagen, Timo L.M. ten; Ursem, Nicolette; Poelmann, R. E.; Westerweel, J.; Hierck, Beerend P.

doi:10.1016/j.jbiomech.2005.03.015

Cited by 178 publications

(178 citation statements)

References 34 publications

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“…The zinc finger transcription factor Krü ppel-Like Factor-2 (KLF2/LKLF) belongs to the family of Sp/XKLF transcription factors, is uniquely expressed in vascular endothelial cells, is induced by high levels of shear stress, and is repressed by low and disturbed flow (Groenendijk et al, 2004;Dekker et al, 2002;Wang et al, 2006). We showed that already during embryonic development, patterning of KLF2 gene expression relates to areas of high shear stress (Groenendijk et al, 2004;Vennemann et al, 2006). Experimental changes in embryonic blood flow lead to changes in gene expression patterning of KLF2, ET1, and eNOS (Groenendijk et al, 2005), and lead to loss of proper cardiovascular function (Stekelenburg-de Vos et al, 2005Ursem et al, 2004) and the development of structural anomalies (Hogers et al, 1997(Hogers et al, , 1999.…”

Section: Discussionmentioning

confidence: 99%

Primary cilia sensitize endothelial cells for fluid shear stress

Hierck

Heiden

Alkemade

et al. 2008

Developmental Dynamics

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Primary cilia are mechanosensors for fluid shear stress, and are involved in a number of syndromes and congenital anomalies. We identified endothelial cilia in areas of low shear stress in the embryonic heart. The objective of the present study was to demonstrate the role of primary cilia in mechanosensing. Ciliated embryonic endothelial cells were cultured from the heart, and non-ciliated cells from the arteries. Nonciliated cells that were subjected to fluid shear stress showed significantly less induction of the shear marker Krü ppel-Like Factor-2, as compared to ciliated cells. In addition, ciliated cells from which the cilia were chemically removed show a similar decrease in flow response. This shows that primary cilia sensitize endothelial cells for fluid shear stress. In addition, we targeted and stabilized the connection of the cilium to the cytoplasm by treatment with Colchicine and Taxol/Paclitaxel, respectively, and show that microtubular integrity is essential to sense shear stress. Developmental Dynamics 237:725-735, 2008.

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Section: Discussionmentioning

confidence: 99%

Primary cilia sensitize endothelial cells for fluid shear stress

Hierck

Heiden

Alkemade

et al. 2008

Developmental Dynamics

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157

108

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“…By using a microchannel close to a rectangular shape, Bitsch and his co-workers (Bitsch et al, 2005) have reported blunt profiles. More recently, by using liposomes tracer particles the bloodplasma velocity was measured in the beating heart of a chicken embryo (Vennemann et al, 2006). Kim and Lee (2006) have analysed the flow behaviour of blood through a circular opaque microchannel by using an X-ray PIV technique.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Blood Flow Behaviour in Microchannels with Simple and Complex Geometries

Garcia¹,

Dias²,

Lima³

2012

Applied Biological Engineering - Principles and Practice

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“…The most plausible explanation relies on the formation of a cell-free plasma layer near the wall, with red blood cells (RBCs) tending to migrate toward the center of the microtube. Extensive research ensued on the flow properties of blood in both dilute and concentrated suspensions using several measuring techniques such as double-slit photometry 7,8 , video microscopy and image analysis [4][5][6]9 , laser-Doppler anemometry 10,11 , and particle-measuring methods [12][13][14][15] . Although extensive studies have been conducted on the microhemodynamic behavior of single RBCs in dilute suspensions, their behavior in concentrated suspensions remains poorly understood, in part due to technical limitations.…”

Section: Introductionmentioning

confidence: 99%

Measurement of Individual Red Blood Cell Motions Under High Hematocrit Conditions Using a Confocal Micro-PTV System

et al. 2009

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Developments in optical experimental techniques have helped in elucidating how blood flows through microvessels. Although initial developments were encouraging, studies on the flow properties of blood in microcirculation have been limited by several technical factors, such as poor spatial resolution and difficulty obtaining quantitative detailed measurements at such small scales. Recent advances in computing, microscopy, and digital image processing techniques have made it possible to combine a particle tracking velocimetry (PTV) system with a confocal microscope. We document the development of a confocal micro-PTV measurement system for capturing the dynamic flow behavior of red blood cells (RBCs) in concentrated suspensions. Measurements were performed at several depths through 100-µm glass capillaries. The confocal micro-PTV system was able to detect both translational and rotational motions of individual RBCs flowing in concentrated suspensions. Our results provide evidence that RBCs in dilute suspensions (3% hematocrit) tended to follow approximately linear trajectories, whereas RBCs in concentrated suspensions (20% hematocrit) exhibited transversal displacements of about 2% from the original path. Direct and quantitative measurements indicated that the plasma layer appeared to enhance the fluctuations in RBC trajectories owing to decreased obstruction in transversal movements caused by other RBCs. Using optical sectioning and subsequent image contrast and resolution enhancement, the system provides previously unobtainable information on the motion of RBCs, including the trajectories of two or more RBCs interacting in the same focal plane and RBC dispersion coefficients in different focal planes.

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In vivo micro particle image velocimetry measurements of blood-plasma in the embryonic avian heart

Cited by 178 publications

References 34 publications

Primary cilia sensitize endothelial cells for fluid shear stress

Primary cilia sensitize endothelial cells for fluid shear stress

In Vitro Blood Flow Behaviour in Microchannels with Simple and Complex Geometries

Measurement of Individual Red Blood Cell Motions Under High Hematocrit Conditions Using a Confocal Micro-PTV System

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