In Vivo Measurement of Gastric Mucus pH in Canines: Effect of High Luminal Acidity and Prostaglandin E2

Patronella, Christopher K.; Vanĕk, I; Bowen, John C.

doi:10.1016/s0016-5085(88)80005-2

Cited by 23 publications

(14 citation statements)

References 13 publications

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“…In a study in which the pH of the gastric surface was measured with a Ag/AgCI microelectrode in dogs, progressive luminal acidification resulted in a decreasing lumen-mucosa pH gradient (Patronella, Vanek and Bowen 1988). However, after progressive luminal acidification to pH 1.0 over 120 mins, the mucosal pH abruptly increased from 1.0 -1.5 to 4.0-7.4.…”

Section: Discussionmentioning

confidence: 99%

Regional gastric pH measurement in horses and foals

Murray

Grodinsky

1989

Equine Veterinary Journal

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Summary The pH of the gastric mucosal surface and gastric content was measured in 18 foals (mean age: 20 days) and 27 horses (mean age: 2.9 years) with a pH electrode passed through an endoscope biopsy channel. A reference electrode was attached to a shaved area on the neck. pH Measurements of the gastric mucosal surface at the dorsal squamous fundus (SF), squamous mucosa adjacent to the margo plicatus (MP), glandular fundus (GF) and the fluid or feed contents of the stomach were recorded in duplicate for each animal. In adult horses, the SF pH was greatest (5.46±1.82), with a decreasing pH ventrally toward the MP (4.12±1.62), to the glandular fundic mucosa (3.09±1.90), and fluid contents (2.72±1.86). The differences in pH at each site within the stomach were significant (P<0.05). In foals, mean pH measurement of the SF was 4.88±1.30, the MP was 4.92±1.29, the GF was 2.10±1.45, milk and fluid contents was 1.85±0.53 (six foals), and feed contents was 3.39±1.77 (12 foals). The pH of the SF and MP were significantly greater (P<0.05) than that of feed contents, which was significantly greater (P<0.05) than that of the glandular mucosal surface and fluid contents. The results indicate a dorsal to ventral pH gradient of the gastric mucosal surface in adult horses, and that gastric acid secretion is competent in young foals.

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Section: Discussionmentioning

confidence: 99%

Regional gastric pH measurement in horses and foals

Murray

Grodinsky

1989

Equine Veterinary Journal

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“…">Chief (zymogenic) cells: secrete pepsinogen. Parietal or oxyntic cells: Secrete hydrochloric acid (HCl) to facilitate protein digestion and activate digesting enzymes (e.g., pepsinogens) that become active in the stomach's acid environment and intrinsic factor, essential for vitamin B12 absorption from the small intestine. Neck mucous cells: mucous secreting cells (mucous layer pH 4–6 compared to stomach acid pH 1–2)6 to protect the gastric lining from damage by stomach acids. Argentaffin cells (Enterochromaffin‐like cells): endocrine cells secreting a variety of hormones including histamine, gastrin, motilin, substance P, vasoactive intestinal polypeptide and somatostatin. Antral pylorus region : These glands are very extensive, secreting an alkaline mucous.…”

Section: Introductionmentioning

confidence: 99%

“…Neck mucous cells: mucous secreting cells (mucous layer pH 4–6 compared to stomach acid pH 1–2)6 to protect the gastric lining from damage by stomach acids.…”

Section: Introductionmentioning

confidence: 99%

Factors Influencing the Gastric Residence of Dosage Forms in Dogs

Martinez

Papich

2009

Journal of Pharmaceutical Sciences

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“…The pH gradient protects the gastric mucosa against back-diffusing acid from the lumen. Earlier studies of the pH gradient and the pH at the gastric epithelial cell surface (pH jm ) were generally conducted in a non-acid-secreting situation, and a total collapse of the gradient was observed when the luminal pH decreased below 1.4 (8,40). However, luminal acidity is a result of endogenous acid secretion, and investigation of the pH gradient during stimulation of acid secretion therefore seems highly relevant.…”

Section: What Is the Ph At The Epithelial Cell Surface During Acid Sementioning

confidence: 99%

Acid Transport through Gastric Mucus

Phillipson

2004

Upsala Journal of Medical Sciences

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The gastric mucosa is frequently exposed to endogenously secreted hydrochloric acid of high acidity. Gastric mucosal defense mechanisms are arranged at different levels of the gastric mucosa and must work in unison to maintain its integrity.In this work, several mechanisms underlying gastric mucosal resistance to strong acid were investigated in anesthetized rats and mice. The main findings were as follows:Only when acid secretion occurred did the pH gradient in the mucus gel withstand back-diffusion of luminal acid (100 mM or 155 mM HCl), and keep the juxtamucosal pH (pHjm) neutral. Thus, with no on-going acid secretion and low luminal pH, the pH gradient was destroyed.Bicarbonate ions, produced concomitant with hydrogen ions in the parietal cells during acid secretion and transported by the blood to the surface epithelium, were carried transepithelially through a DIDS-sensitive transport. Prostaglandin-dependent bicarbonate secretion seemed to be less important in maintaining a neutral pH jm .Removal of the loosely adherent mucus layer did not influence the maintenance of the pH jm . Hence, only the firmly adherent mucus gel layer, approximately 80 µm thick, seemed to be important for the pH jm .Staining of the mucus gel with a pH-sensitive dye revealed that secreted acid penetrated the mucus gel from the crypt openings toward the gastric lumen only in restricted paths (channels). One crypt opening was attached to one channel, and the channel was irreversibly formed during acid secretion.Gastric mucosal blood flow increased on application of strong luminal acid (155 mM HCl). This acid-induced hyperemia involved the inducible but not the neural isoform of nitric oxide synthase. These results suggest a novel role for iNOS in gas-1

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In Vivo Measurement of Gastric Mucus pH in Canines: Effect of High Luminal Acidity and Prostaglandin E2

Cited by 23 publications

References 13 publications

Regional gastric pH measurement in horses and foals

Regional gastric pH measurement in horses and foals

Factors Influencing the Gastric Residence of Dosage Forms in Dogs

Acid Transport through Gastric Mucus

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