In vivo measurement of F420 fluorescence in cultures of Methanobacterium thermoautotrophicum

Reuter, Bernd; Egeler, T.; Schneckenburger, Herbert; Schoberth, Siegfried M.

doi:10.1016/0168-1656(86)90046-5

Cited by 17 publications

(7 citation statements)

References 11 publications

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“…The final extracellular concentration of coenzyme F420 and FO reached 270 nM with M. barkeri grown on methanol and is considerably less than the value of approximately 5.8 FM previously recorded for M. thermoautotrophicum (18). The higher concentration noted with M. thermoautotrophicium may reflect a greater amount of coenzyme F420 present in this methanogen (10,14), a larger proportion (70 to 90%) of total fluorescence in the supernatant (27), and a higher cell concentration. The relative proportions of the coenzyme F420 analogs were similar in the cells and supernatant with the exception of FO, which was principally located in the culture supernatant.…”

Section: Discussionmentioning

confidence: 88%

Changes in concentrations of coenzyme F420 analogs during batch growth of Methanosarcina barkeri and Methanosarcina mazei

Peck

1989

Appl Environ Microbiol

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Coenzyme F420 has been assayed by high-performance liquid chromatography with fluorimetric detection; this permits quantification of individual coenzyme F420 analogs whilst avoiding the inclusion of interfering material. The total intracellular coenzyme F420 content of Methanosarcina barkeri MS cultivated on methanol and on H2-C02 and of Methanosarcina mazei S-6 cultured on methanol remained relatively constant during batch growth. The most abundant analogs in M. barkeri were coenzymes F420-2 and F420-4, whilst in M. mazei coenzymes F420-2 and F420-3 predominated. Significant changes in the relative proportions of the coenzyme F420 analogs were noted during batch growth, with coenzymes F420-2 and F420-4 showing opposite responses to each other and the same being also true for coenzymes F420-3 and F420-5. This suggests that an enzyme responsible for transferring pairs of glutamic acid residues may be active. The degradation fragment FO was also detected in cells in late exponential and stationary phase. Coenzyme F420 analogs were present in the culture supernatant of both methanogens, in similar proportions to that in the cells, except for FO which was principally located in the supernatant.

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Section: Discussionmentioning

confidence: 88%

Changes in concentrations of coenzyme F420 analogs during batch growth of Methanosarcina barkeri and Methanosarcina mazei

Peck

1989

Appl Environ Microbiol

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“…The method of fluorimetric determination of F420 (as also applied in this paper) yields the total concentration of this coenzyme. Recently, it was suggested that the methanogenic activity should be assessed by measuring only the oxidized part of F420 (22).…”

Section: Discussionmentioning

confidence: 99%

Relationship of Intracellular Coenzyme F ₄₂₀ Content to Growth and Metabolic Activity of Methanobacterium bryantii and Methanosarcina barkeri

Heine-Dobbernack¹,

Schoberth²,

Sahm³

1988

Appl Environ Microbiol

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The use of F420 as a parameter for growth or metabolic activity of methanogenic bacteria was investigated. Two representative species of methanogens were grown in batch culture: Methanobacterium bryantii (strain M.o.H.G.) on H2 and CO2, and Methanosarcina barkeri (strain Fusaro) on methanol or acetate. The total intracellular content of coenzyme F420 was followed by high-resolution fluorescence spectroscopy. F420 concentration in M. bryantii ranged from 1.84 to 3.65 ,Lmol g of protein-'; and in M. barkeri grown with methanol it ranged from 0.84 to 1.54 ,umol g-depending on growth conditions. The content of F420 in M. barkeri was influenced by a factor of 2 depending on the composition of the medium (minimal or complex) and by a factor of 3 to 4 depending on whether methanol or acetate was used as the carbon source. A comparison of F420 content with protein, cell dry weight, optical density, and specific methane production rate showed that the intracellular content of F420 approximately followed the increase in biomass in both strains. In contrast, no correlation was found between specific methane production rate and intracellular F420 content. However, qCH4(F420), calculated by dividing the methane production rate by the coenzyme F420 concentration, almost paralleled qCH4(protein). These results suggest that F420 may be used as a specific parameter for estimating the biomass, but not the metabolic activity, of methanogens; hence qCH4(F420) determined in mixed populations with complex carbon substrates must be considered as measure of the actual methanogenic activity and not as a measure of potential activity.

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“…Pottered and sliced granules were examined with a Zeiss Axioplan 2 epifluorescence microscope equipped with filter set 06 (bp 436/10 FT 460). To visualize methanogens in these samples, the fluorescence of coenzyme F 420 , present in most methanogens, was used as described by Reuter et al ( 1986 ).…”

Section: Methodsmentioning

confidence: 99%

Effect and behaviour of different substrates in relation to the formation of aerobic granular sludge

Pronk

Abbas

Al-Zuhairy

et al. 2015

Appl Microbiol Biotechnol

155

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When aerobic granular sludge is applied for industrial wastewater treatment, different soluble substrates can be present. For stable granular sludge formation on volatile fatty acids (e.g. acetate), production of storage polymers under anaerobic feeding conditions has been shown to be important. This prevents direct aerobic growth on readily available chemical oxygen demand (COD), which is thought to result in unstable granule formation. Here, we investigate the impact of acetate, methanol, butanol, propanol, propionaldehyde, and valeraldehyde on granular sludge formation at 35 °C. Methanogenic archaea, growing on methanol, were present in the aerobic granular sludge system. Methanol was completely converted to methane and carbon dioxide by the methanogenic archaeum Methanomethylovorans uponensis during the 1-h anaerobic feeding period, despite the relative high dissolved oxygen concentration (3.5 mg O2 L−1) during the subsequent 2-h aeration period. Propionaldehyde and valeraldehyde were fully disproportionated anaerobically into their corresponding carboxylic acids and alcohols. The organic acids produced were converted to storage polymers, while the alcohols (produced and from influent) were absorbed onto the granular sludge matrix and converted aerobically. Our observations show that easy biodegradable substrates not converted anaerobically into storage polymers could lead to unstable granular sludge formation. However, when the easy biodegradable COD is absorbed in the granules and/or when the substrate is converted by relatively slow growing bacteria in the aerobic period, stable granulation can occur.Electronic supplementary materialThe online version of this article (doi:10.1007/s00253-014-6358-3) contains supplementary material, which is available to authorized users.

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In vivo measurement of F420 fluorescence in cultures of Methanobacterium thermoautotrophicum

Cited by 17 publications

References 11 publications

Changes in concentrations of coenzyme F420 analogs during batch growth of Methanosarcina barkeri and Methanosarcina mazei

Changes in concentrations of coenzyme F420 analogs during batch growth of Methanosarcina barkeri and Methanosarcina mazei

Relationship of Intracellular Coenzyme F ₄₂₀ Content to Growth and Metabolic Activity of Methanobacterium bryantii and Methanosarcina barkeri

Effect and behaviour of different substrates in relation to the formation of aerobic granular sludge

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In vivo measurement of F420 fluorescence in cultures of Methanobacterium thermoautotrophicum

Cited by 17 publications

References 11 publications

Changes in concentrations of coenzyme F420 analogs during batch growth of Methanosarcina barkeri and Methanosarcina mazei

Changes in concentrations of coenzyme F420 analogs during batch growth of Methanosarcina barkeri and Methanosarcina mazei

Relationship of Intracellular Coenzyme F 420 Content to Growth and Metabolic Activity of Methanobacterium bryantii and Methanosarcina barkeri

Effect and behaviour of different substrates in relation to the formation of aerobic granular sludge

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Relationship of Intracellular Coenzyme F ₄₂₀ Content to Growth and Metabolic Activity of Methanobacterium bryantii and Methanosarcina barkeri