In Vivo Loose-Patch-Juxtacellular Labeling of Cerebellar Neurons in Mice

Cerebellar Cortex 4–12 Hz Oscillations and Unit Phase Relation in the Awake Rat

“…For Purkinje cells, it is customary to confirm SS identity with the co-recording of complex spikes (Welsh et al, 1999 ; Gao et al, 2011 ), which we did not systematically do here, focusing on obtaining both strong GCL LFP oscillations and well-isolated units. For any type of cell, the method of juxtacellular labeling using micropipettes is also quite advantageous in identifying cell types that are recorded from (Barmack and Yakhnitsa, 2008 ; Brown et al, 2018 ) but this exceeded the scope of our experimental methods. Here, we used the cell’s location, its action potential as well as its firing pattern properties, especially the relation between the firing rate and its variability, as done previously (Vos et al, 1999 ).…”