In Vivo Localization of Gelatinases (MMP-2 and -9) by in Situ Zymography with a Selective Gelatinase Inhibitor

Pirilä, Emma; Maisi, Päivi; Salo, Tuula; Koivunen, Erkki; Sorsa, Timo

doi:10.1006/bbrc.2001.5653

Cited by 39 publications

(45 citation statements)

References 37 publications

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“…The CTT peptide has also been used to modify the natural tropism of adenovirus for a therapeutic gene delivery in a rabbit restenosis model (Turunen et al, 2002). In addition, CTT peptide has been used to localize gelatinase activity in tissue samples using in situ zymography (Pirilä et al, 2001), and to evaluate the contribution of gelatinases in various biological processes including vasoconstriction, epithelial-mesenchymal transition and hepatitis (Cheng and Lovett, 2003;Fernandez-Patron et al, 2000;Franzke et al, 2002).…”

Section: Synthetic Gelatinase Inhibitorsmentioning

confidence: 99%

Gelatinase-mediated migration and invasion of cancer cells

Björklund

Koivunen

2005

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer

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609

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Section: Synthetic Gelatinase Inhibitorsmentioning

confidence: 99%

Gelatinase-mediated migration and invasion of cancer cells

Björklund

Koivunen

2005

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer

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463

609

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“…Immunohistological stainings were performed using standard procedures and antibodies as described previously (38). Paraffin-embedded tissue specimens were deparaffinized, and immunostainings for MMP-9 (R&D Systems, Minneapolis, MN), MMP-13 (Chemicon, Temecula, CA), MMP-25 (Sigma-Aldrich Co., St. Louis, MO), COX-1 (Santa Cruz Biotechnology, Santa Cruz, CA), COX-2 (Santa Cruz Biotechnology), myeloperoxidase (MPO) (HyCult Biotechnology, b.v., Uden, The Netherlands), laminin-332 (32), neutrophil elastase (NE; Calbiochem-Novabiochem, San Diego, CA), interleukin-1␤ (IL-1␤; R&D Systems), and tumor necrosis factor alpha (TNF-␣; R&D Systems) were performed as described previously (30).…”

Section: Animalsmentioning

confidence: 99%

Local and Systemic Responses in Matrix Metalloproteinase 8-Deficient Mice duringPorphyromonas gingivalis-Induced Periodontitis

et al. 2009

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Periodontitis is a bacterium-induced chronic inflammation that destroys tissues that attach teeth to jaw bone. Pathologically excessive matrix metalloproteinase 8 (MMP-8) is among the key players in periodontal destruction by initiating type I collagen degradation. We studied MMP-8 in Porphyromonas gingivalis-induced periodontitis by using MMP-8-deficient (MMP8 ؊/؊ ) and wild-type (WT) mice. Alveolar bone loss, inflammatory mediator expression, serum immunoglobulin, and lipoprotein responses were investigated to clarify the role of MMP-8 in periodontitis and systemic inflammatory responses. P. gingivalis infection induced accelerated site-specific alveolar bone loss in both MMP8؊/؊ and WT mice relative to uninfected mice. The most extensive bone degradation took place in the P. gingivalis-infected MMP8 ؊/؊ group. Surprisingly, MMP-8 significantly attenuated (P < 0.05) P. gingivalis-induced site-specific alveolar bone loss. Increased alveolar bone loss in P. gingivalis-infected MMP8 ؊/؊ and WT mice was associated with increase in gingival neutrophil elastase production. Serum lipoprotein analysis demonstrated changes in the distribution of high-density lipoprotein (HDL) and very-low-density lipoprotein (VLDL) particles; unlike the WT mice, the MMP8 ؊/؊ mice underwent a shift toward a smaller HDL/VLDL particle sizes. P. gingivalis infection increased the HDL/VLDL particle size in the MMP8 ؊/؊ mice, which is an indicator of lipoprotein responses during systemic inflammation. Serum total lipopolysaccharide activity and the immunoglobulin G-class antibody level in response to P. gingivalis were significantly elevated in both infected mice groups. Thus, MMP-8 appears to act in a protective manner inhibiting the development of bacterium-induced periodontal tissue destruction, possibly through the processing anti-inflammatory cytokines and chemokines. Bacterium-induced periodontitis, especially in MMP8 ؊/؊ mice, is associated with systemic inflammatory and lipoprotein changes that are likely involved in early atherosclerosis.

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“…The latter approach was taken, first using a newly developed specific peptide inhibitor of gelatinases (57,86). Cyclic CTTHWGFTLC (cyclic CTT) has some preference for inhibiting MMP-2 relative to MMP-9 and is 10-fold more potent than STTHWGFTLS (STT).…”

Section: And Citations Therein)mentioning

confidence: 99%

Emerging role of relaxin in renal and cardiovascular function

Conrad

Novak

2004

American Journal of Physiology-Regulatory, Integrative and Comp

133

113

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Conrad, Kirk P., and Jacqueline Novak. Emerging role of relaxin in renal and cardiovascular function. Am J Physiol Regul Integr Comp Physiol 287: R250 -R261, 2004; 10.1152/ajpregu.00672.2003.-Although traditionally associated with reproductive processes, relaxin is emerging as an important player in renal and cardiovascular function. Much of our recently acquired understanding of relaxin in this new context has arisen from studies of maternal renal and cardiovascular adaptations to pregnancy in rats where the hormone is turning out to be an important mediator. First, we highlight the influence of relaxin on renal hemodynamics and glomerular filtration rate, as well as on other peripheral circulations. Second, we discuss the effect of relaxin on both the steady and pulsatile systemic arterial load, as well as on the heart, in particular, coronary blood flow. Third, we consider the impact of the hormone on cultured endothelial and vascular smooth muscle cells. Fourth, we address the interaction of relaxin with renal and cardiac disease, as well as its role in angiogenesis. Finally, in Perspectives, we point out several key research questions in need of investigation that relate to a potential autocrine/paracrine role of relaxin in renal and cardiovascular tissues. Furthermore, on the basis of its potent vasodilatory and matrix-degrading attributes, we speculate about the therapeutic potential of relaxin in renal and cardiovascular diseases. pregnancy; kidney circulation; glomerular filtration; osmoregulation; peripheral circulation; systemic hemodynamics; vasodilation; arterial compliance; heart; myogenic reactivity; angiogenesis; relaxin receptors; matrix metalloproteinase; gelatinase; endothelin B receptor; nitric oxide; angiotensin II THE OBJECTIVE OF THIS REVIEW is to highlight the new and emerging roles of relaxin (RLX) in the renal and cardiovascular systems. Traditionally, RLX has been tied to reproductive processes during pregnancy, because most investigations conducted by reproductive biologists have dealt with the hormone in this context. The traditional sources of RLX have been reproductive tissues such as the corpus luteum of the ovary from which the hormone emanates and circulates during the luteal phase of the menstrual cycle and during pregnancy in women (95). Furthermore, there has been no well-established role in the male. However, the concept of a role for RLX in cardiovascular function actually has its antecedents early on in the history of RLX research, when Frederick L. Hisaw, who discovered the hormone (47, 113), noted that after administration of RLX to castrated monkeys, there were marked morphological changes in the endothelial cells of the endometrium consistent with hypertrophy and hyperplasia (48). The concept that RLX can affect blood vessel structure and function has since gained considerable support, particularly in the last decade. Much of our recently gained knowledge of RLX in this new light has arisen from studies of maternal renal and cardiovascular function in the gravid rat mo...

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In Vivo Localization of Gelatinases (MMP-2 and -9) by in Situ Zymography with a Selective Gelatinase Inhibitor

Cited by 39 publications

References 37 publications

Gelatinase-mediated migration and invasion of cancer cells

Gelatinase-mediated migration and invasion of cancer cells

Local and Systemic Responses in Matrix Metalloproteinase 8-Deficient Mice duringPorphyromonas gingivalis-Induced Periodontitis

Emerging role of relaxin in renal and cardiovascular function

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