In Vivo Inhibition of Murine Leukemia and Sarcoma Viruses by the Heteropolyanion 5-Tungsto-2-antimoniate2

Jasmin, C; Chermann, Jean‐Claude; Hervé, Guy; Tézé, A.; Souchay, Pierre; Boy-Loustau, Christiane; Raybaud, N.; Sinoussi, F. Barre; Raynaud, M

doi:10.1093/jnci/53.2.469

Cited by 117 publications

(33 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No mechanism for these reactions has been shown. An Sb polytungstate, (NHg)a 7 Na [NaSb 9 W 21 08 6 ]" 14H2 O, exhibits activity against various virus induced tumours [251] including Friend leukaemia and Moloney sarcoma virus [260] and the mechanism of action may involve inhibition of the viral polymerase by the anion [251].…”

Section: Antiviral Activitymentioning

confidence: 99%

The structure and reactivity of arsenic compounds: Biological activity and drug design

Dhubhghaill

Sadler

1991

Structure and Bonding

View full text Add to dashboard Cite

Section: Antiviral Activitymentioning

confidence: 99%

The structure and reactivity of arsenic compounds: Biological activity and drug design

Dhubhghaill

Sadler

1991

Structure and Bonding

View full text Add to dashboard Cite

“…These compounds have been shown to exhibit antiviral activity both in vitro and in vivo at noncytotoxic doses against various DNA and RNA viruses. For example, HPA protects mice against lethal infections induced by retroviruses such as Friend leukemia virus [2,3] and murine mammary tumor virus [4], and nononcogenic viruses such as vesicular stomatitis virus [2] and encephalomyocarditis virus [2]. The compound has also been shown to be effective against rabies virus multiplication [5] as well as experimental scrapie in mice [6].…”

mentioning

confidence: 99%

Differential inhibition of various mammalian DNA polymerase activities by ammonium 21‐tungsto‐9‐antimoniate (HPA23)

Ono

Nakane

Barré‐Sinoussi

et al. 1988

European Journal of Biochemistry

Self Cite

View full text Add to dashboard Cite

The 21-tungsto-Pantimoniate ammonium salt (HPA23), known as an antiviral agent, has been shown to be a potent inhibitor of both human and murine DNA polymerase CI and murine DNA polymerase y. HPA23 inhibited the activity of DNA polymerase CI in noncompetitive fashion with respect to either deoxynucleotide substrate or nucleic acid template . primer. The Ki of murine DNA polymerase c1 for HPA23 was determined to be 24 nM. The activity of mouse DNA polymerase y also was strongly inhibited by HPA23 (Ki, 20 nM), and the mode of inhibition was competitive with respect to the template . primer, (rA)n . (dT)12--18, and noncompetitive to substrate, dTTP. DNA polymerase fl and terminal deoxynucleotidyltransferase, however, were relatively resistant to inhibition by HPA23. The observed inhibitions by HPA23 seem to be closely related to the polyanionic property of this drug.Tungstic heteropolyanions (HPAs) belong to a family of polyoxotungstates of a cryptate structure with an alkaline or alkaline-earth cation in the central cage and negative charges on all oxygen atoms [l]. These compounds have been shown to exhibit antiviral activity both in vitro and in vivo at noncytotoxic doses against various DNA and RNA viruses. For example, HPA protects mice against lethal infections induced by retroviruses such as Friend leukemia virus [2,3] and murine mammary tumor virus [4], and nononcogenic viruses such as vesicular stomatitis virus [2] and encephalomyocarditis virus [2]. The compound has also been shown to be effective against rabies virus multiplication [5] as well as experimental scrapie in mice [6]. A systematic examination of a number of HPAs revealed that one of them, ammonium 21-tungsto-9-antimoniate (HPA23), had the strongest antiviral activity [7].The exact mechanism of antiviral action of HPA is not yet known. However, ammonium 5-tungsto-2-antimoniate inhibited AMV and MLV reverse transcriptases [8, 91 and Abbreviations. HPA, heteropolyanion; AMV, avian myeloblastosis virus; MLV, murine leukemia virus; IdUrd, 5-iodo-2'-deoxyuridine; terminal transferase, terminal deoxynucleotidyltransferase; RLV, Rauscher leukemia virus; HIV, human immundeficiency virus.Enzymes. DNA polymerase, deoxynucleoside triphosphate: DNA deoxynucleotidyltransferase (EC 2.7.7.7); terminal deoxynucleotidyltransferase (EC 2.7.7.31); reverse transcriptase (EC 2.7.7.49). associated reverse transcriptase, since the enzyme activity was strongly inhibited in the presence of the drug [12]. In spite of these findings, specificity of the inhibition by HPA23 of various eukaryotic DNA polymerases has remained to be elucidated.Our recent works on the modulation of Epstein-Barr virus gene expression in a human lymphoblastoid cell line (Raji) have revealed that HPA23 inhibited early antigen expression induced by 5-iodo-2'-deoxyuridine (IdUrd) [13]. Treatment of Raji cells with HPA23 caused a marked decrease in DNA polymerase CI activity which could presumably result in an inhibition of IdUrd incorporation leading to the reduction of early antigen expression ...

show abstract

“…All these studies employed a regime of 9 to 12 daily doses of HPA-23 (the first two doses were given before scrapie inoculation) based on a regimen successfully used against murine leukemia and sarcoma viruses (19). We developed an effective three-dose regime to define the optimum times between HPA-23 treatment and scrapie infection.…”

mentioning

confidence: 99%

Suppression of scrapie infection in mice by heteropolyanion 23, dextran sulfate, and some other polyanions

Kimberlin¹,

Walker²

1986

Antimicrob Agents Chemother

133

View full text Add to dashboard Cite

Studies of polyanions that suppress scrapie have been done to pinpoint the cell types in the lymphoreticular system which are important in pathogenesis and to suggest possible prophylactic or therapeutic strategies for the unconventional slow viruses. A regime of three daily injections of the inorganic heteropolyanion HPA-23 reduced the effective scrapie dose by more than 99%; i.e., some mice survived peripherally injected doses of 100 50% lethal dose units. The effect was greatest when the first dose of HPA-23 was given 4 h ifter injecting scrapie, but it declined rapidly as this interval was increased, and there was virtually no effect 2 days after infection. A single dose of high-molecular-weight organic polyanions such as carrageenan or dextran sulfate (DS-500) greatly reduced (i.e., >99%) the efficiency of scrapie infection. In contrast to HPA-23, DS-500 was equally effective whether given 4 days before or 8 h after the time of infection. The antiscrapie effect of DS-500 appeared to be independent of its activity as a B-cell mitogen and of its ability to produce a cytotoxic blockade of phagocytic cells. DS-500 probably caused the aggregation and loss from blood of scrapie inoculum which was present immediately after injection, but it had additional effects on scrapie at later times.

show abstract

In Vivo Inhibition of Murine Leukemia and Sarcoma Viruses by the Heteropolyanion 5-Tungsto-2-antimoniate2

Cited by 117 publications

References 0 publications

The structure and reactivity of arsenic compounds: Biological activity and drug design

The structure and reactivity of arsenic compounds: Biological activity and drug design

Differential inhibition of various mammalian DNA polymerase activities by ammonium 21‐tungsto‐9‐antimoniate (HPA23)

Suppression of scrapie infection in mice by heteropolyanion 23, dextran sulfate, and some other polyanions

Contact Info

Product

Resources

About