In vivo immunoregulatory properties of the novel mitochondria-targeted antioxidant SkQ1

Yang, Yuhui; Karakhanova, Svetlana; Soltek, Sabine; Werner, Jens; Philippov, Pavel P.; Bazhin, Alexandr V.

doi:10.1016/j.molimm.2012.04.010

Cited by 24 publications

(27 citation statements)

References 33 publications

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“…C57BL/6 or B7-H1KO mice at the age of 8-12 weeks were used for the study. Blood was collected under anesthesia by the cardiac puncher; afterward the mice were sacrificed and their spleens removed and used for preparation of single-cell splenocyte suspensions [33].…”

Section: Micementioning

confidence: 99%

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Immunological in vivo effects of B7-H1 deficiency

et al. 2014

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Section: Micementioning

confidence: 99%

“…Flow cytometry (FACS) analysis of murine splenocytes was performed as described previously [33]. Freshly isolated spleen cell suspension was resuspended in the stain buffer (PBS supplemented with 1% mouse serum and 1 mM EDTA).…”

Section: Flow Cytometry Analysis Of Murine Splenocytesmentioning

confidence: 99%

Immunological in vivo effects of B7-H1 deficiency

et al. 2014

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“…The mice were sacrificed 21 d after the cell implantation and their tumors were removed to prepare single cell suspensions as described elsewhere. 39 Flow cytometry analysis of MDSC in peripheral blood of PDAC patients…”

Section: Cell Culturementioning

confidence: 99%

Characterization of myeloid leukocytes and soluble mediators in pancreatic cancer: importance of myeloid-derived suppressor cells

et al. 2015

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Pancreatic ductal adenocarcinoma (PDAC) represents one of the deadliest cancers in the world. PDAC cells activate tumor-specific immune responses but simultaneously trigger a strong immunosuppression. We showed that PDAC cells produce high amount of chronic inflammatory mediators and PDAC tumors build an immunosuppressive cytokine milieu, which correlates with tumor progression. We observed a low frequency of dendritic cells (DC) and a pronounced accumulation of macrophages and myeloid-derived suppressor cells (MDSC) in murine PDAC tumors. A strong accumulation of MDSC has also been demonstrated in the peripheral blood of resected PDAC patients. While DC and macrophages seem not to play a significant role in this PDAC model in the context of immunosuppression, MDSC are highly suppressive, and their accumulation is associated with an increase in intratumoral VEGF concentration during the PDAC progression. Application of the phosphodiesterase-5 inhibitor sildenafil led to a prolonged survival of PDACbearing female mice, which was due to the decrease in MDSC frequencies and in the systemic VEGF level. This led to a restoration of anticancer immune responses, manifested in the recovery of T lymphocyte functions and in an increase in the frequency of conventional CD4 C T cells in tumors and IFNg level in serum of PDAC-bearing mice. Thus, MDSC are strongly involved in the PDAC-associated immunosuppression and that their depletion could create new approaches for therapy of PDAC.

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“…Flow cytometry (FACS) analysis of murine splenocytes was performed as described elsewhere [21]. Briefly, single cell suspensions of spleens were prepared in the StainBuffer (PBS supplemented with 1% mouse serum and 1 mM EDTA).…”

Section: Flow Cell Biology Analysis Of Murine Splenocyte Samplesmentioning

confidence: 99%

“…After 24 h cultivation the cells were analyzed by FACS (Fig. S2) using our established murine panels [21]. The IFN treatment of splenocytes led to an increase in the frequency of lymphocytes in the cultivated cells (Fig.…”

Section: Influence Of Chemotherapeutics On Splenocytes From Tumor-beamentioning

confidence: 99%

In vitro immunomodulatory properties of gemcitabine alone and in combination with interferon-alpha

et al. 2015

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In vivo immunoregulatory properties of the novel mitochondria-targeted antioxidant SkQ1

Cited by 24 publications

References 33 publications

Immunological in vivo effects of B7-H1 deficiency

Immunological in vivo effects of B7-H1 deficiency

Characterization of myeloid leukocytes and soluble mediators in pancreatic cancer: importance of myeloid-derived suppressor cells

In vitro immunomodulatory properties of gemcitabine alone and in combination with interferon-alpha

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