In vivo imaging using polymeric nanoparticles stained with near-infrared chemiluminescent and fluorescent squaraine catenane endoperoxide

Lee, Jung-Jae; White, Alexander G.; Rice, Douglas R.; Smith, Bradley D.

doi:10.1039/c3cc40630j

Cited by 41 publications

(35 citation statements)

References 31 publications

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“…Therefore, alternative approaches to PDT have been attempted using a new type of excitation that employs light generated inside of the body by CL instead of using an external light source [138,139]. The endogenous light generation by CL is also useful for autofluorescence-free diagnostic imaging with high sensitivity.As an example, thermally controllable polymeric NPs were devised for in vivo imaging [140,141]. Two types of dyes, squaraine rotaxane endoperoxide (SREP) and squaraine catenane endoperoxide (SCEP), were examined for both CL and fluorescence imaging (Fig.…”

Section: Chemi-and Bioluminescence-based Imaging and Photodynamic Thementioning

confidence: 99%

Nanoparticles of Conjugated Molecules and Polymers for Biomedical Applications

Seo

Lee

Cho

et al. 2015

Advances in Polymer Science

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Semiconducting organic materials based on π-conjugated molecules or polymers have recently received considerable attention for their use as imaging and therapeutic agents for biomedical applications. Because π-conjugated materials exhibit desirable characteristics such as high brightness, photostability, emission tunability, and low toxicity, they are an attractive alternative to conventional fluorophores. In this review, the recent development of π-conjugated molecular nanoparticles for in vitro and in vivo biomedical applications is discussed.

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Section: Chemi-and Bioluminescence-based Imaging and Photodynamic Thementioning

confidence: 99%

Nanoparticles of Conjugated Molecules and Polymers for Biomedical Applications

Seo

Lee

Cho

et al. 2015

Advances in Polymer Science

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“…Somewhat surprisingly, the utility of this approach as an intraoperative imaging technique has remained unexplored 10 . Indeed, the closest example to this technique is the oxidation of luminol by myeloperoxidase in mice 11,12,13 . Chemiluminescent biomedical imaging is therefore a rather unexplored area of research that could offer the following advantages: (1) minimal autofluorescence resulting in a low background signal with higher signal-to-noise ratios; (2) tunable wavelengths of chemiluminescent emissions ranging from the visible to the near-infrared; and (3) functionalizable chemiluminescent complexes that, when combined with linker technologies and targeted biomolecules that already exist, provide access to whole libraries of targeted molecular imaging probes 14 .…”

Section: Introductionmentioning

confidence: 99%

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

Büchel

Carney

Tang

et al. 2017

JoVE

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Intraoperative imaging techniques have the potential to make surgical interventions safer and more effective; for these reasons, such techniques are quickly moving into the operating room. Here, we present a new approach that utilizes a technique not yet explored for intraoperative imaging: chemiluminescent imaging. This method employs a ruthenium-based chemiluminescent reporter along with a custom-built nebulizing system to produce ex vivo or in vivo images with high signal-to-noise ratios. The ruthenium-based reporter produces light following exposure to an aqueous oxidizing solution and re-reduction within the surrounding tissue. This method has allowed us to detect reporter concentrations as low as 6.9 pmol/cm2. In this work, we present a visual guide to our proof-of-concept in vivo studies involving subdermal and intravenous injections in mice. The results suggest that this technology is a promising candidate for further preclinical research and might ultimately become a useful tool in the operating room.

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“…Compared to systemic delivery by an intravenous route, tracking organ-specific delivery of NP vehicles is demanding, as an additional compartment must be included in any pharmacokinetic analysis. We and others have shown that NPs accumulate within the airways and alveolar air sacs, providing a potential depot effect for cargo release, and also impeding clearance or degradation that relies on extra-pulmonary metabolism or excretion[34, 35]. …”

Section: Introductionmentioning

confidence: 99%

In vivo fate tracking of degradable nanoparticles for lung gene transfer using PET and Ĉerenkov imaging

et al. 2016

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Nanoparticles (NPs) play expanding roles in biomedical applications including imaging and therapy, however, their long-term fate and clearance profiles have yet to be fully characterized in vivo. NP delivery via the airway is particularly challenging, as the clearance may be inefficient and lung immune responses complex. Thus, specific material design is required for cargo delivery and quantitative, noninvasive methods are needed to characterize NP pharmacokinetics. Here, biocompatible poly(acrylamidoethylamine)-b-poly(DL-lactide) block copolymer-based degradable, cationic, shell-cross-linked knedel-like NPs (Dg-cSCKs) were employed to transfect plasmid DNA. Radioactive and optical beacons were attached to monitor biodistribution and imaging. The preferential release of cargo in acidic conditions provided enhanced transfection efficiency compared to non-degradable counterparts. In vivo gene transfer to the lung was correlated with NP pharmacokinetics by radiolabeling Dg-cSCKs and performing quantitative biodistribution with parallel positron emission tomography and Čerenkov imaging. Quantitation of imaging over 14 days corresponded with the pharmacokinetics of NP movement from the lung to gastrointestinal and renal routes, consistent with predicted degradation and excretion. This ability to noninvasively and accurately track NP fate highlights the advantage of incorporating multifunctionality into particle design.

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In vivo imaging using polymeric nanoparticles stained with near-infrared chemiluminescent and fluorescent squaraine catenane endoperoxide

Cited by 41 publications

References 31 publications

Nanoparticles of Conjugated Molecules and Polymers for Biomedical Applications

Nanoparticles of Conjugated Molecules and Polymers for Biomedical Applications

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

In vivo fate tracking of degradable nanoparticles for lung gene transfer using PET and Ĉerenkov imaging

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