In-vivo imaging of psoriatic lesions with polarization multispectral dermoscopy and multiphoton microscopy

Kapsokalyvas, Dimitrios; Cicchi, Riccardo; Bruscino, Nicola; Alfieri, Domenico; Prignano, Francesca; Massi, Daniela; Lotti, Torello; Pavone, Francesco S.

doi:10.1364/boe.5.002405

Cited by 35 publications

(30 citation statements)

References 35 publications

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“…Kapsokalyvas et al (126) presented a multiphoton microscope and a polarization multispectral dermoscope (PMD) situated in the clinics. Both are optimized for in vivo measurements of skin.…”

Section: Multiphoton/multimodal Nonlinear Optical Microscopymentioning

confidence: 99%

Multimodal Imaging Spectroscopy of Tissue

Vogler

Heuke

Bocklitz

et al. 2015

Annual Rev. Anal. Chem.

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Advanced optical imaging technologies have experienced increased visibility in medical research, as they allow for a label-free and nondestructive investigation of tissue in either an excised state or living organisms. In addition to a multitude of ex vivo studies proving the applicability of these optical imaging approaches, a transfer of various modalities toward in vivo diagnosis is currently in progress as well. Furthermore, combining optical imaging techniques, referred to as multimodal imaging, allows for an improved diagnostic reliability due to the complementary nature of retrieved information. In this review, we provide a summary of ongoing multifold efforts in multimodal tissue imaging and focus in particular on in vivo applications for medical diagnosis. We also discuss the advantages and potential limitations of the imaging methods and outline opportunities for future developments.

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“…Kapsokalyvas et al (126) presented a multiphoton microscope and a polarization multispectral dermoscope (PMD) situated in the clinics. Both are optimized for in vivo measurements of skin.…”

Section: Multiphoton/multimodal Nonlinear Optical Microscopymentioning

confidence: 99%

Multimodal Imaging Spectroscopy of Tissue

Vogler

Heuke

Bocklitz

et al. 2015

Annual Rev. Anal. Chem.

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“…The volumes are rotated 30° in the y - z plane. (g) The papillae in healthy skin are short, typically 30 μ m, and with small diameter, around 29 μ m. (h) Psoriatic papillae dimensions are bigger with typical lengths more than 100 μ m and a diameter of around 65 μ m. (Scale bars: 50 μ m.) Figure modified from [55]. …”

Section: Figurementioning

confidence: 99%

Clinical Nonlinear Laser Imaging of Human Skin: A Review

Cicchi

Kapsokalyvas

Pavone

2014

BioMed Research International

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Nonlinear optical microscopy has the potential of being used in vivo as a noninvasive imaging modality for both epidermal and dermal imaging. This paper reviews the capabilities of nonlinear microscopy as a noninvasive high-resolution tool for clinical skin inspection. In particular, we show that two-photon fluorescence microscopy can be used as a diagnostic tool for characterizing epidermal layers by means of a morphological examination. Additional functional information on the metabolic state of cells can be provided by measuring the fluorescence decay of NADH. This approach allows differentiating epidermal layers having different structural and cytological features and has the potential of diagnosing pathologies in a very early stage. Regarding therapy follow-up, we demonstrate that nonlinear microscopy could be successfully used for monitoring the effect of a treatment. In particular, combined two-photon fluorescence and second-harmonic generation microscopy were used in vivo for monitoring collagen remodeling after microablative fractional laser resurfacing and for quantitatively monitoring psoriasis on the basis of the morphology of epidermal cells and dermal papillae. We believe that the described microscopic modalities could find in the near future a stable place in a clinical dermatological setting for quantitative diagnostic purposes and as a monitoring method for various treatments.

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“…Such morpho‐functional approach represents a powerful tool for tissue characterization and pathology assessment. Finally, the high penetration of near infrared wavelengths (typically ~500 μm at 840 nm ) allows maintaining high spatial resolution when imaging in thick tissue, and hence the possibility to image both thin slices and thick samples . In fact, there is a consolidated literature on NLO applications to the study of atherosclerotic plaques .…”

Section: Introductionmentioning

confidence: 99%

Improved label‐free diagnostics and pathological assessment of atherosclerotic plaques through nonlinear microscopy

Baria

Nesi

Santi

et al. 2018

Journal of Biophotonics

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Coronary heart disease is the most common type of heart disease caused by atherosclerosis. In fact, an arterial wall lesion centered on the accumulation of cholesterol-rich lipids and the accompanying inflammatory response generates a plaque, whose rupture may result in a thrombus with fatal consequences. Plaque characterization for assessing the severity of atherosclerosis is generally performed through standard histopathological examination based on hematoxylin/eosin staining, which is operator-dependent and requires relatively long procedures. In this framework, nonlinear optical microscopy is a valid, label-free alternative to standard diagnostic methods. We combined second-harmonic generation (SHG), two-photon excited fluorescence (TPEF) and fluorescence lifetime imaging microscopy in a multimodal scheme for obtaining morphological and molecular information on human carotid ex vivo specimens affected by atherosclerosis. In this study, discrimination between different tissues within the atherosclerotic plaque was achieved based on both lifetime, TPEF-to-SHG ratio, and image pattern analysis. The presented methodology aims to be a starting point for future fully automated and fast characterization of atherosclerotic biopsies; moreover, it could be extended to the study of other tissues and pathologies. Combined TPEF/SHG mapping of a carotid specimen affected by atherosclerosis.

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In-vivo imaging of psoriatic lesions with polarization multispectral dermoscopy and multiphoton microscopy

Cited by 35 publications

References 35 publications

Multimodal Imaging Spectroscopy of Tissue

Multimodal Imaging Spectroscopy of Tissue

Clinical Nonlinear Laser Imaging of Human Skin: A Review

Improved label‐free diagnostics and pathological assessment of atherosclerotic plaques through nonlinear microscopy

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