In Vivo Imaging of Mouse Cochlea by Optical Coherence Tomography

Tona, Yosuke; Sakamoto, Tatsunori; Nakagawa, Takayuki; Adachi, Takuji; Taniguchi, Mirei; Torii, Hiroko; Hamaguchi, Kozo; Ito, Juichi

doi:10.1097/mao.0000000000000252

Cited by 19 publications

(15 citation statements)

References 25 publications

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“…The novel improvements in imaging technologies resulted in the introduction of experimental imaging modalities to evaluate the intracochlear microanatomy such as OCT and SLOT (7,(11)(12)(13)(14). These high resolution modalities are nondestructive and facilitate near real time evaluation of the structures.…”

Section: Discussionmentioning

confidence: 99%

Preparation of Human Inner Ear Structures for High Resolution Imaging Studies

et al. 2017

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Background: Introduction of the novel imaging modalities such as optical coherence tomography and scanning laser optical tomography allow the morphological and functional evaluation of the intracochlear structures up to the histological details. The prerequisite of these modalities is the preparation of the membranous labyrinth by removing the dense otic capsule to enhance the optical penetration depth. In the present study, a combination of the chemical decalcification and bone drilling method was explained for the preparation of the human inner ear structures for further studies. Methods: In this study, nine human temporal bones were used and trimmed in cubes containing middle and inner ear structures. The samples were immersed in 5% nitric acid, 20% EDTA, and 10% EDTA solutions, respectively. The samples were brought out and rinsed every 90 minutes, and mechanical removal of the softened bone was performed with surgical drill system until the complete decalcification of the samples was confirmed by X-ray imaging. The prepared samples were evaluated by microcomputed tomography imaging for anatomical distortions caused by the preparation process.

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Section: Discussionmentioning

confidence: 99%

Preparation of Human Inner Ear Structures for High Resolution Imaging Studies

et al. 2017

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“…The feasibility of OCT for quantitative assessment of the endolymphatic space, organ of Corti, and lateral wall was evaluated by comparison with H&E-stained images using a surgically induced EH model, 11 a model of deafness induced by kanamycin-ethacrynic acid, 24 and a model of strial atrophy induced by streptomycin sulfate perfusion. 25 Unlike the procedure utilized in the study by Tona et al, 17 in this study, cochlear OCT images were acquired in vitro after decalcification using EDTA to achieve a high resolution. In the current study, 3-D reconstruction of the OCT images was performed and the hearing status was evaluated at different time points after surgical induction of EH.…”

Section: Discussionmentioning

confidence: 99%

“…Tona et al reported that OCT is useful for the evaluation of EH using Slc26A4 (−/−) mice in vivo. 17 In that study, the second turn was chosen to estimate EH because of the limited penetration depth of OCT. The hearing level was estimated and OCT images and histological findings were compared in Slc26A4 (−/−) , Slc26A4 (+/−) , and Slc26A4 (+/+) mice.…”

Section: Discussionmentioning

confidence: 99%

“…It is difficult to identify EH in patients with Meniere's disease because clinical examinations such as the auditory brainstem response (ABR) and caloric tests only provide functional information regarding a damaged cochlea or vestibule. Consequently, many studies have attempted to investigate the pathophysiology of Meniere's disease and the presence/absence of EH using histological examination in animal models, [11][12][13][14][15][16][17] with the most popular model being that induced by surgical ablation of the endolymphatic duct and sac. A surgical technique proposed by Kimura requires penetration of the brain's protective covering, leading to an increase in morbidity and mortality associated with penetration of the central nervous system.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the usefulness of three-dimensional optical coherence tomography in a guinea pig model of endolymphatic hydrops induced by surgical obliteration of the endolymphatic duct

et al. 2015

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Abstract. Optical coherence tomography (OCT) has advanced significantly over the past two decades and is currently used extensively to monitor the internal structures of organs, particularly in ophthalmology and dermatology. We used ethylenediamine tetra-acetic acid (EDTA) to decalcify the bony walls of the cochlea and investigated the inner structures by deep penetration of light into the cochlear tissue using OCT on a guinea pig model of endolymphatic hydrops (EH), induced by surgical obliteration of the endolymphatic duct. The structural and functional changes associated with EH were identified using OCT and auditory brainstem response tests, respectively. We also evaluated structural alterations in the cochlea using three-dimensional reconstruction of the OCT images, which clearly showed physical changes in the cochlear structures. Furthermore, we found significant anatomical variations in the EH model and conducted graphical analysis by strial atrophy for comparison. The physical changes included damage to and flattening of the organ of Corti-evidence of Reissner's membrane distention-and thinning of the lateral wall. These results indicate that observation of EDTA-decalcified cochlea using OCT is significant in examination of gradual changes in the cochlear structures that are otherwise not depicted by hematoxylin and eosin staining.

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“…The use of local contrasting agent has started to enable this visualization [4]. Attempts to visualize cochlear structure in vivo using a technique known as optical coherence tomography (OCT) have been made with success [5,6].…”

Section: Hyd =mentioning

confidence: 99%

Estimation of the Degree of Endolymphatic Hydrops Using Optical Coherence Tomography

Karvonen

Uranishi

Sakamoto

et al. 2016

ABE

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Endolymphatic hydrops is a disorder in which an excessive amount of endolymph uid causes an increase in the pressure of the endolymphatic system of the inner ear. In cochlea, endolymphatic hydrops can cause stretching in the scala media, a tubular construction that, along with two other similar structures, the scala vestibuli and scala tympani, runs through the cochlea from the base to the top. Visualizing the enlargement of the scala media has traditionally been done by histological study of tissue sections, and the degree can be calculated by comparing the ratio of the area of the scala media to the combined total area of the three compartments. However, this requires the obtainment of tissue samples, and thus is not suited for in vivo measurements, and is a labor intensive process in general. Imaging modalities that have been used to measure endolymphatic hydrops include MRI, μMRI and μCT. Recently, optical coherence tomography (OCT) has been applied for visualizing cochlea of mice in vivo. However, just visually discriminating between healthy and af icted cochlea, and judging the degree of af iction can be dif cult. Thus, we aim to establish a new technique for estimating the degree of enlargement of the scala media. We measured two healthy and two diseased cochlea using conventional area calculation, as well as our method, and present results that show that our technique correlates with established standards for measuring the degree of endolymphatic hydrops in cochlea (R = 0.977, p = 0.5 × 10 −7).

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In Vivo Imaging of Mouse Cochlea by Optical Coherence Tomography

Abstract: OCT can be applied in the detection of endolymphatic hydrops in living mice, indicating the potential of OCT for cochlear imaging analyses for clinical use in the near future.

Cited by 19 publications

References 25 publications

Preparation of Human Inner Ear Structures for High Resolution Imaging Studies

Preparation of Human Inner Ear Structures for High Resolution Imaging Studies

Evaluation of the usefulness of three-dimensional optical coherence tomography in a guinea pig model of endolymphatic hydrops induced by surgical obliteration of the endolymphatic duct

Estimation of the Degree of Endolymphatic Hydrops Using Optical Coherence Tomography

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