In vivo imaging of functional disruption, recovery and alteration in rat olfactory circuitry after lesion

Cross, Donna; Flexman, Jennifer A.; Anzai, Yoshimi; Morrow, Thomas; Maravilla, Kenneth R.; Minoshima, Shinsei

doi:10.1016/j.neuroimage.2006.04.229

Cited by 30 publications

(24 citation statements)

References 37 publications

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“…3B, arrow); (2) signiˆcantly enhanced regions and their order of detection on the t-maps of Group A (Fig. 3A, Table 1) that support theˆndings of Cross and associates 13,14 ; (3) similar regions of signiˆcant enhancement and extent of enhancement in Groups A and B at 48 and 72 hours and 7 days (Figs. 3-5, Table 1), which show that verapamil did not aŠect the pathway to transport Mn; and (4) initial detection of signiˆcant enhancement from Mn 2＋ 48 hours after MnCl 2 administration in Group B (Fig.…”

Section: Voxel-based Comparison and Roi Analysissupporting

confidence: 67%

“…[13][14][15][16][17][18] With these techniques, spatial normalization of the brain permits voxel-based analysis of the entire brain and more objective results than those of conventional ROI/VOI analysis. The results are less susceptible to changes in subject shape and size, and position, shape, and size of the ROI/VOI do not vary between observers.…”

Section: Magnetic Resonance In Medical Sciencesmentioning

confidence: 99%

“…[13][14][15] These methods using MEMRI are expected to become a basic and important method for rat brain research, so their applicability for such investigation requires validation. We applied Statistical Parametric Mapping 8 software (SPM8; Wellcome Department of Cognitive Neurology, London, UK [http://www.ˆl.ion.…”

Section: Magnetic Resonance In Medical Sciencesmentioning

confidence: 99%

“…Cross's group have reported investigations of functional transport of Mn in the olfactory system of normal rats, 13 alterations in function transport after injury, 14 and age-related decrease in axonal transport 15 with the statistical mapping method using MEMRI. In this study, we investigated the eŠect of a calcium-channel blocker (verapamil) on the transport of Mn in olfactory connections using the statistical method with MEMRI.…”

Section: Voxel-based Comparison and Roi Analysismentioning

confidence: 99%

“…An FWHM value that is too large for smoothing prevents detection of small regions enhanced by Mn 2＋ , and one that is too small reduces the statistical power, as described. Because recent studies made no reference to the smoothing parameter for statistical mapping of the rat brain using MEMRI, [11][12][13][14][15][16][17][18] we set the parameter such that the t-score was increased. Figure 7 shows that smoothing increased the t-score.…”

Section: Increasing Statistical Power By Smoothingmentioning

confidence: 99%

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Statistical Parametric Mapping for Effects of Verapamil on Olfactory Connections of Rat Brain in Vivo using Manganese-enhanced MR Imaging

et al. 2011

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Purpose: We investigated the eŠect of verapamil on the transport of manganese in the olfactory connections of rat brains in vivo using statistical parametric mapping and manganese-enhanced magnetic resonance (MR) imaging.Methods: We divided 12 7-week-old male Sprague-Dawley rats into 2 groups of six and injected 10 mL of saline into the right nasal cavities of theˆrst group and 10 mL of verapamil (2.5 mg/mL) into the other group. Twenty minutes after the initial injection, we injected 10 mL of MnCl 2 (1 mol/L) into the right nasal cavities of both groups. We obtained serial T 1 -weighted MR images before administering the verapamil or saline and at 0.5, one, 24, 48, and 72 hours and 7 days after administering the MnCl 2 , spatially normalized the MR images on the rat brain atlas, and analyzed the data using voxel-based statistical comparison.Results: Statistical parametric maps demonstrated the transport of manganese. Manganese ions created signiˆcant enhancement (t-score＝36.6) 24 hours after MnCl 2 administration in the group administered saline but not at the same time point in the group receiving verapamil. The extent of signiˆcantly enhanced regions peaked at 72 hours in both groups and both sides of the brain. The peak of extent in the right side brain in the group injected with saline was 70.2 mm 3 and in the group with verapamil, 92.4 mm 3 . The extents in the left side were 64.0 mm 3 for the group with saline and 53.2 mm 3 for the group with verapamil.Conclusion: We applied statistical parametric mapping using manganese-enhanced MR imaging to demonstrate in vivo the transport of manganese in the olfactory connections of rat brains with and without verapamil and found that verapamil did aŠect this transport.

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Section: Voxel-based Comparison and Roi Analysissupporting

confidence: 67%

Section: Magnetic Resonance In Medical Sciencesmentioning

confidence: 99%

Section: Magnetic Resonance In Medical Sciencesmentioning

confidence: 99%

Section: Voxel-based Comparison and Roi Analysismentioning

confidence: 99%

Section: Increasing Statistical Power By Smoothingmentioning

confidence: 99%

See 3 more Smart Citations

Statistical Parametric Mapping for Effects of Verapamil on Olfactory Connections of Rat Brain in Vivo using Manganese-enhanced MR Imaging

et al. 2011

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Assessing transneuronal dysfunction utilizing manganese‐enhanced MRI (MEMRI)

Serrano

Deshazer

Smith

et al. 2008

Magnetic Resonance in Med

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In this study we utilized manganese-enhanced MRI (MEMRI) to evaluate the in vivo transneuronal efficiency of manganese ion (Mn 2؉ ) movement as a means to assess overall changes in neuronal function. We designated this extension the manganese transfer index (MTI) value. To evaluate the MTI value as an index of transneuronal physiology we examined both pharmacological agents and different mouse models of neuronal dysfunction. We found that treatment with isoflurane, which attenuates synaptic vesicle release, or memantine, which attenuates postsynaptic uptake of Ca 2؉ as well as Mn 2؉

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Measurement of neuro‐energetics and neurotransmission in the rat olfactory bulb using ¹H and ¹H–[¹³C] NMR spectroscopy

et al. 2023

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The olfactory bulb (OB) plays a fundamental role in the sense of smell and has been implicated in several pathologies, including Alzheimer's disease. Despite its importance, high metabolic activity and unique laminar architecture, the OB is not frequently studied using MRS methods, likely due to the small size and challenging location. Here we present a detailed metabolic characterization of OB metabolism, in terms of both static metabolite concentrations using 1H MRS and metabolic fluxes associated with neuro‐energetics and neurotransmission by tracing the dynamic 13C flow from intravenously administered [1,6‐13C2]‐glucose, [2‐13C]‐glucose and [2‐13C]‐acetate to downstream metabolites, including [4‐13C]‐glutamate, [4‐13C]‐glutamine and [2‐13C]‐GABA. The unique laminar architecture and associated metabolism of the OB, distinctly different from that of the cerebral cortex, is characterized by elevated GABA and glutamine levels, as well as increased GABAergic and astroglial energy metabolism and neurotransmission. The results show that, despite the technical challenges, high‐quality 1H and 1H–[13C] MR spectra can be obtained from the rat OB in vivo. The derived metabolite concentrations and metabolic rates demonstrate a unique metabolic profile for the OB. The metabolic model provides a solid basis for future OB studies on functional activation or pathological conditions.

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In vivo imaging of functional disruption, recovery and alteration in rat olfactory circuitry after lesion

Cited by 30 publications

References 37 publications

Statistical Parametric Mapping for Effects of Verapamil on Olfactory Connections of Rat Brain in Vivo using Manganese-enhanced MR Imaging

Statistical Parametric Mapping for Effects of Verapamil on Olfactory Connections of Rat Brain in Vivo using Manganese-enhanced MR Imaging

Assessing transneuronal dysfunction utilizing manganese‐enhanced MRI (MEMRI)

Measurement of neuro‐energetics and neurotransmission in the rat olfactory bulb using ¹H and ¹H–[¹³C] NMR spectroscopy

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In vivo imaging of functional disruption, recovery and alteration in rat olfactory circuitry after lesion

Cited by 30 publications

References 37 publications

Statistical Parametric Mapping for Effects of Verapamil on Olfactory Connections of Rat Brain in Vivo using Manganese-enhanced MR Imaging

Statistical Parametric Mapping for Effects of Verapamil on Olfactory Connections of Rat Brain in Vivo using Manganese-enhanced MR Imaging

Assessing transneuronal dysfunction utilizing manganese‐enhanced MRI (MEMRI)

Measurement of neuro‐energetics and neurotransmission in the rat olfactory bulb using 1H and 1H–[13C] NMR spectroscopy

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Measurement of neuro‐energetics and neurotransmission in the rat olfactory bulb using ¹H and ¹H–[¹³C] NMR spectroscopy