In vivo imaging of cancer biomarkers using activatable molecular probes

Targeted gene therapy and in vivo bioluminescent imaging for monitoring postsurgical recurrence and metastasis in mouse models of liver cancer

“…By inserting the luciferase gene into the targeted gene promoter, this gene becomes the reporter gene, thereby enabling the in vivo monitoring of the targeted gene (Hoehn, 2015). The technique has considerable advantages over traditional detection methods (Elias et al, 2008).…”

Metabolomics Research with Tandem Mass Spectrometry

“…The vital progress of metabolomics usually occurs when new instrumentation or technique appears. Various state of the art techniques have been applied to metabolomics studies, including: nuclear magnetic resonance (NMR) spectroscopy (Reo, 2002;Ward et al, 2007;Jordan and Cheng, 2007;Serkova et al, 2007;Duarte et al, 2009;Maher et al, 2009;Sofia et al, 2011), magnetic resonance imaging (MRI) (Elias et al, 2008;De Leon-Rodriguez et al, 2009), infrared spectroscopy (IR) (Singh and Sinclair, 2007;Shaw et al, 2009;Corte et al, 2010), gas chromatography mass spectrometry (GC-MS) (Styczynski et al, 2007;Xu et al, 2009;Lin et al, 2010;Dehaven et al, 2010;Carroll et al, 2010;Tsugawa et al, 2011), capillary electrophoresis-MS (Lee et al, 2007;Soga, 2007;Lapainis et al, 2009;Barbas et al, 2011;BritzMcKibbin, 2011) and HPLC-MS Lu et al, 2008;Llorach et al, 2009;Gika and Theodoridis, 2011;Bajad and Shulaev, 2011) . Current metabolomics and metabolic profiling studies rely almost exclusively on 1 H NMR, GC-MS and LC-MS due to the technological maturity of these instrumentations.…”

Developing Probes for Molecular Imaging

“…For example, imaging neuroendocrine tumors with peptides targeting somatostatin receptors such as DOTA-TOC, -NOC or -octreotide as well radiolabeled bombesin targeting the gastrin releasing peptide receptor have been used in humans, some of them such as octreotide are part of our clinical routine. These Nuclear Medicine approaches provide a nearly unsurpassed sensitivity (though optical imaging can get close), however, imaging probes for other modalities offer opportunities to image molecular interactions that are not physically possible with nuclides (Elias et al 2008). Such probes include activatable or smart sensing imaging agents, which comprise the most complex of all imaging probes (Fig.…”