In vivo imaging in experimental spinal cord injury – Techniques and trends

Hubertus, Vanessa; Meyer, Léa; Roolfs, Laurens; Waldmann, Lilly; Nieminen-Kelhä, Melina; Fehlings, Michael G.; Vajkoczy, Peter

doi:10.1016/j.bas.2021.100859

Cited by 3 publications

(1 citation statement)

References 144 publications

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“…Other methods to track cell migration include magnetic resonance imaging, in vivo microscopy, and micro-computed tomography. However, these methods require expensive equipment, making results reproducibility difficult, especially in low-income countries [31]. The myelin sheath, produced by oligodendrocytes in the central nervous system, renders the structure to the spinal cord and regulates neuronal growth through neurite outgrowth inhibitor (NOGO) molecules secreted by injured or dysfunctional oligodendrocytes.…”

Section: Discussionmentioning

confidence: 99%

Human Adipose-Derived Stem Cells Reduce Cellular Damage after Experimental Spinal Cord Injury in Rats

et al. 2023

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Traumatic spinal cord injury (SCI) is a devastating condition without an effective therapy. Cellular therapies are among the promising treatment strategies. Adult stem cells, such as mesenchymal stem cells, are often used clinical research for their immunomodulatory and regenerative potential. This study aimed to evaluate the effect of human adipose tissue-derived stem cells (ADSC) infusion through the cauda equina in rats with SCI. The human ADSC from bariatric surgery was isolated, expanded, and characterized. Wistar rats were subjected to blunt SCI and were divided into four groups. Two experimental groups (EG): EG1 received one ADSC infusion after SCI, and EG2 received two infusions, the first one after SCI and the second infusion seven days after the injury. Control groups (CG1 and CG2) received infusion with a culture medium. In vivo, cell tracking was performed 48 h and seven days after ADSC infusion. The animals were followed up for 40 days after SCI, and immunohistochemical quantification of myelin, neurons, and astrocytes was performed. Cellular tracking showed cell migration towards the injury site. ADSC infusion significantly reduced neuronal loss, although it did not prevent the myelin loss or enhance the area occupied by astrocytes compared to the control group. The results were similar when comparing one or two cell infusions. The injection of ADSC distal to the injured area was shown to be a safe and effective method for cellular administration in spinal cord injury.

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Section: Discussionmentioning

confidence: 99%

Human Adipose-Derived Stem Cells Reduce Cellular Damage after Experimental Spinal Cord Injury in Rats

et al. 2023

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Organization of collagen fibers and tissue hardening: Markers of fibrotic scarring after spinal cord injury in mice revealed by multiphoton-atomic force microscopy imaging

Manesco

Saavedra-Villanueva

Martin

et al. 2023

Nanomedicine: Nanotechnology, Biology and Medicine

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Perspectives on endoscopic functional photoacoustic microscopy

Yang,

2024

Applied Physics Letters

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Endoscopy, enabling high-resolution imaging of deep tissues and internal organs, plays an important role in basic research and clinical practice. Recent advances in photoacoustic microscopy (PAM), demonstrating excellent capabilities in high-resolution functional imaging, have sparked significant interest in its integration into the field of endoscopy. However, there are challenges in achieving functional PAM in the endoscopic setting. This Perspective article discusses current progress in the development of endoscopic PAM and the challenges related to functional measurements. Then, it points out potential directions to advance endoscopic PAM for functional imaging by leveraging fiber optics, microfabrication, optical engineering, and computational approaches. Finally, it highlights emerging opportunities for functional endoscopic PAM in basic and translational biomedicine.

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In vivo imaging in experimental spinal cord injury – Techniques and trends

Cited by 3 publications

References 144 publications

Human Adipose-Derived Stem Cells Reduce Cellular Damage after Experimental Spinal Cord Injury in Rats

Human Adipose-Derived Stem Cells Reduce Cellular Damage after Experimental Spinal Cord Injury in Rats

Organization of collagen fibers and tissue hardening: Markers of fibrotic scarring after spinal cord injury in mice revealed by multiphoton-atomic force microscopy imaging

Perspectives on endoscopic functional photoacoustic microscopy

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