In vivo IgA coating of anaerobic bacteria in human faeces.

Waaij, Laurens A. van der; Limburg, P. C.; Mesander, G; Waaij, D. van der

doi:10.1136/gut.38.3.348

Cited by 190 publications

(128 citation statements)

References 28 publications

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“…Coating of bacteria has been previously observed in feces samples recovered from human and mouse (12,41), suggesting that this takes place at all times in the gut lumen. We made the assumption that nonspecific SIgA serving as substitute of natural SIgA can bind to commensals in a Fab-independent fashion.…”

Section: Siga Binds Bacteria In a Fab-and Fc-independent Manner-mentioning

confidence: 96%

“…The biological function of such SIgA remains speculative; however, various studies have underscored the natural coating of commensal bacteria by SIgA, a process that may be involved in the homeostatic gut sensing of the microbiota (12,41,44).…”

Section: Discussionmentioning

confidence: 99%

“…One can speculate that interaction between SIgA and commensals plays a role in modulating the colonization by the microbiota in steady-state conditions. Moreover, in vivo coating of commensal bacteria by SIgA has been described in analysis of human feces (12,13). Because abundant intestinal secretion of natural SIgA with unknown specificity has also been described, we speculated that the latter can be involved in binding to commensals (8, 14 -16).…”

mentioning

confidence: 99%

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Recognition of Gram-positive Intestinal Bacteria by Hybridoma- and Colostrum-derived Secretory Immunoglobulin A Is Mediated by Carbohydrates

Mathias

Corthésy

2011

Journal of Biological Chemistry

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Section: Siga Binds Bacteria In a Fab-and Fc-independent Manner-mentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Recognition of Gram-positive Intestinal Bacteria by Hybridoma- and Colostrum-derived Secretory Immunoglobulin A Is Mediated by Carbohydrates

Mathias

Corthésy

2011

Journal of Biological Chemistry

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“…Time course experiments showed that there is no dependence of the final concentration of bacteria on adhesion. 3 Because intestinal bacteria have been shown to be coated with a mixture of specific and nonspecific, "natural" SIgA (19,20), we sought to examine 3 B. Corthé sy, unpublished results. whether the combination of LPR and BL with SIgA might have influenced the adhesion properties of the bacteria.…”

Section: Adhesion Of Lpr Alone Bl Alone or In Complexes Withmentioning

confidence: 99%

Potentiation of Polarized Intestinal Caco-2 Cell Responsiveness to Probiotics Complexed with Secretory IgA

Mathias

Duc

Favre

et al. 2010

Journal of Biological Chemistry

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The precise mechanisms underlying the interaction between intestinal bacteria and the host epithelium lead to multiple consequences that remain poorly understood at the molecular level. Deciphering such events can provide valuable information as to the mode of action of commensal and probiotic microorganisms in the gastrointestinal environment. Potential roles of such microorganisms along the privileged target represented by the mucosal immune system include maturation prior, during and after weaning, and the reduction of inflammatory reactions in pathogenic conditions. Using human intestinal epithelial Caco-2 cell grown as polarized monolayers, we found that association of a Lactobacillus or a Bifidobacterium with nonspecific secretory IgA (SIgA) enhanced probiotic adhesion by a factor of 3.4-fold or more. Bacteria alone or in complex with SIgA reinforced transepithelial electrical resistance, a phenomenon coupled with increased phosphorylation of tight junction proteins zonula occludens-1 and occludin. In contrast, association with SIgA resulted in both enhanced level of nuclear translocation of NF-B and production of epithelial polymeric Ig receptor as compared with bacteria alone. Moreover, thymic stromal lymphopoietin production was increased upon exposure to bacteria and further enhanced with SIgA-based complexes, whereas the level of pro-inflammatory epithelial cell mediators remained unaffected. Interestingly, SIgA-mediated potentiation of the Caco-2 cell responsiveness to the two probiotics tested involved Fab-independent interaction with the bacteria. These findings add to the multiple functions of SIgA and underscore a novel role of the antibody in interaction with intestinal bacteria.

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“…However it has also been reported that commensal bacteria can elicit systemic antibody responses (Kimura et al, 1997). In addition a proportion of the resident microflora is covered by antibodies in the lumen of the gut, mainly of the IgA isotype (Van der Waaij et al, 1996).…”

Section: Interactions Between the Host And The Intestinal Microfloramentioning

confidence: 99%

Interactions between the microbiota and the intestinal mucosa

2002

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The intestinal microflora can be considered as a postnatally aquired organ composed of a large diversity of bacterial cells that can perform different functions for the host. This organ is highly exposed to environmental influences and thus modulated in its composition and functions by external factors, such as nutrition. Specific components of the intestinal microflora, including lactobacilli and bifidobacteria, have been associated with beneficial effects on the host, such as promotion of gut maturation and integrity, antagonisms against pathogens and immune modulation. In addition, the microflora seem to play a significant role in the maintenance of intestinal immune homeostasis and prevention of inflammation. At the present time, the contribution of intestinal epithlial cell in the first line of defence against pathogenic bacteria and microbial antigens has been recognized, in contrast, the interactions of intestinal epithelial cells with commensal bacteria are less understood. The present work summarizes the increasing scientific attention for mechanisms of the innate immune response of the host to different components of the autochthonous microflora and suggests a potential role for selected probiotic bacteria in the regulation of intestinal inflammation.

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In vivo IgA coating of anaerobic bacteria in human faeces.

Abstract: The bacterial flora in the human colon, although extremely diverse, has a relatively stable composition and non-infectious anaerobic bacteria are dominant. The

Cited by 190 publications

References 28 publications

Recognition of Gram-positive Intestinal Bacteria by Hybridoma- and Colostrum-derived Secretory Immunoglobulin A Is Mediated by Carbohydrates

Recognition of Gram-positive Intestinal Bacteria by Hybridoma- and Colostrum-derived Secretory Immunoglobulin A Is Mediated by Carbohydrates

Potentiation of Polarized Intestinal Caco-2 Cell Responsiveness to Probiotics Complexed with Secretory IgA

Interactions between the microbiota and the intestinal mucosa

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