In vivo hydrogen peroxide diffusivity in brain tissue supports volume signaling activity

Ledo, Ana; Fernandes, Eliana; Salvador, Armindo; Laranjinha, João; Barbosa, Rui M.

doi:10.1016/j.redox.2022.102250

Cited by 25 publications

(22 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…H 2 O 2 could be detoxified by the activity of CAT, GPX, APX, and GR, as well as by the ascorbate–glutathione cycle. Molecular hydrogen peroxide is relatively stable, and its reaction with most biological molecules is limited (Ledo et al 2022 ). H 2 O 2 is electrically neutral ROS, but it is detrimental because it can cross cell membranes and reach cell parts far from the site of its formation.…”

Section: Discussionmentioning

confidence: 99%

Improving the effects of salt stress by β-carotene and gallic acid using increasing antioxidant activity and regulating ion uptake in Lepidium sativum L.

2022

View full text Add to dashboard Cite

Background Plant growth and development are severely affected by soil salinity. This study was carried out to evaluate the interaction of foliar application of antioxidants (β-carotene and gallic acid) and salt stress on Lepidium sativum seedlings. Results Our findings revealed that total dry and fresh weight were adversely affected by 25 mM NaCl salinity stress. Moreover, K+ content decreased while Na+ content increased significantly. The foliar application of β-carotene and gallic acid significantly mitigated the effects of salt stress by regulating ion uptake, reducing H2O2 and malondialdehyde (MDA) content, as well as increasing enzymatic antioxidant activity, phenolic, glutathione, and chlorophyll content. Conclusions β-carotene- and gallic acid-treated plants had higher salt tolerance.

show abstract

Section: Discussionmentioning

confidence: 99%

Improving the effects of salt stress by β-carotene and gallic acid using increasing antioxidant activity and regulating ion uptake in Lepidium sativum L.

2022

View full text Add to dashboard Cite

show abstract

“…Here we show that a rapid systemic ROS signal can be triggered by a local treatment of high light (HL) stress in Pinus sylvestris (Pine, a Gymnosperm), Azolla filiculoides (Azolla, a fern), and Selaginella moellendorffii (Selaginella, a Lycopodiaceae; Figure 1). The average rate of the ROS signal measured in these organisms is 0.14 cm min −1 (Table S1), faster than the diffusion rate of H2O2 in water or biological systems (the diffusion coefficient of H2O2 in water or tissues is in the range of 1-2.5 10 −5 cm 2 min −1 ) [34][35][36] , supporting the active nature of this systemic ROS signal. The findings presented in Figure 1 suggest that rapid systemic ROS signaling is conserved across several different members of the vascular plant family and may have evolved before these lineages diverged from a common ancestor.…”

Section: Ros As Rapid Systemic Signals In Vascular Plantsmentioning

confidence: 68%

“…A similar directional spread of the ROS signal was also observed in isolated hearts (Figure 8; Movie S2), further supporting the notion that it is not a simple diffusion process. In addition, the rate of the ROS signal observed in hearts was also faster than that of H2O2 in biological systems or water (Table S1) [34][35][36] . Taken together, these findings suggest that the ROS signal observed in a monolayer of H9c2 cells (Figure 7), or isolated hearts (Figure 8), is driven by an active process of ROS production (that could be inhibited by DPI; Figures 7 and 8), involving multiple cells along its path (i.e., not only cells at and around the injury site).…”

Section: Discussionmentioning

confidence: 96%

ROS are evolutionary conserved cell-to-cell signals

Fichman

Rowland

Oliver

et al. 2022

Preprint

View full text Add to dashboard Cite

Cell-to-cell communication is fundamental to multicellular organisms, as well as to unicellular organisms living in a microbiome. It is thought to have evolved as a stress- or quorum-sensing mechanism in unicellular organisms. A unique cell-to-cell communication mechanism that uses reactive oxygen species (ROS) as a signal (termed the ROS wave) was recently identified in flowering plants. This process is essential for systemic signaling and plant acclimation to stress and can spread from a small group of cells to the entire plant within minutes. Whether this signaling process is found in other organisms is however unknown. Here we report that a ROS-mediated cell-to-cell signaling process, like the ROS wave, can be found in ferns, mosses, multicellular and unicellular algae, amoeba, and mammalian cells. We further show that this process can be triggered by extracellular H2O2 application and blocked by inhibition of NADPH oxidases, and that at least in unicellular algae growing as a lawn on an agar plate, it communicates important stress-response signals between cells. Taken together, our findings suggest that an active process of cell-to-cell ROS signaling, like the ROS wave, evolved before unicellular and multicellular organisms diverged. This mechanism could have communicated an environmental stress signal between cells and coordinated the acclimation response of cells living in a community. The finding of a signaling process, like the ROS wave, in mammalian cells further contributes to our understanding of different diseases and could impact the development of new drugs that target, for example cancer or heart disease.

show abstract

“…During neuroinflammation, myeloid cells are proposed as main agents of oxidative damage induced to ODCs through their release of ROS/RNS (Mishra & Yong, 2016; Murray & Wynn, 2011). These radicals have different half‐lives which directly affect their extracellular distribution in the CNS, with peroxide potentially able to diffuse up to 100 μm in distance from its cellular source (Ledo et al, 2022; Santos et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

“…During EAE, ROS/RNS can be produced by both activated CNS‐resident microglia (Simpson & Oliver, 2020) and blood borne monocyte‐derived macrophages (Locatelli et al, 2018), which respectively proliferate and invade the CNS and together contribute to the formation of myeloid‐cell dominated CNS lesions (Jordão et al, 2019; Saederup et al, 2010). Despite the ability of some ROS to diffuse extracellularly to a relatively long distance from their cellular source (Ledo et al, 2022), the high reactivity of radicals will presumably affect cells near ROS/RNS production to a higher degree. At clinical disease onset in ODC mitoGFP‐Tomato mice, ODC mitochondria accordingly displayed: (I) compromised morphology in close proximity to lesions containing Iba1 + microglia/macrophages, and (II) higher oxidation state close to iNOS + microglia/macrophages.…”

Section: Discussionmentioning

confidence: 99%

Autoimmune neuroinflammation triggers mitochondrial oxidation in oligodendrocytes

Steudler

Ecott

Ivan

et al. 2022

Glia

View full text Add to dashboard Cite

Oligodendrocytes (ODCs) are myelinating cells of the central nervous system (CNS) supporting neuronal survival. Oxidants and mitochondrial dysfunction have been suggested as the main causes of ODC damage during neuroinflammation as observed in multiple sclerosis (MS). Nonetheless, the dynamics of this process remain unclear, thus hindering the design of neuroprotective therapeutic strategies. To decipher the spatio-temporal pattern of oxidative damage and dysfunction of ODC mitochondria in vivo, we created a novel mouse model in which ODCs selectively express the ratiometric H 2 O 2 biosensor mito-roGFP2-Orp1 allowing for quantification of redox changes in their mitochondria. Using 2-photon imaging of the exposed spinal cord, we observed significant mitochondrial oxidation in ODCs upon induction of the MS model experimental autoimmune encephalomyelitis (EAE). This redox change became already apparent during the preclinical phase of EAE prior to CNS infiltration of inflammatory cells. Upon clinical EAE development, mitochondria oxidation remained detectable and was associated with a significant impairment in organelle density and morphology. These alterations correlated with the proximity of ODCs to inflammatory lesions containing activated microglia/macrophages. During the chronic progression of EAE, ODC mitochondria maintained an altered morphology, but their oxidant levels decreased to levels observed in healthy mice. Taken together, our study implicates oxidative stress in ODC mitochondria as a novel pre-clinical sign of MS-like inflammation and demonstrates that evolving redox and morphological changes in mitochondria accompany ODC dysfunction during neuroinflammation.

show abstract

In vivo hydrogen peroxide diffusivity in brain tissue supports volume signaling activity

Cited by 25 publications

References 57 publications

Improving the effects of salt stress by β-carotene and gallic acid using increasing antioxidant activity and regulating ion uptake in Lepidium sativum L.

Improving the effects of salt stress by β-carotene and gallic acid using increasing antioxidant activity and regulating ion uptake in Lepidium sativum L.

ROS are evolutionary conserved cell-to-cell signals

Autoimmune neuroinflammation triggers mitochondrial oxidation in oligodendrocytes

Contact Info

Product

Resources

About