In Vivo Hemostatic Effect of the Medicinal Plant Extract Ankaferd Blood Stopper in Rats Pretreated With Warfarin

Cipil, Handan; Koşar, Ali; Kaya, Arif; Uz, Burak; Haznedaroğlu, İbrahim C.; Göker, Hakan; Özdemir, Oktay; Köroğlu, Mustafa; Kirazlı, Şerafettin; Firat, Hüseyin

doi:10.1177/1076029608329578

Cited by 77 publications

(90 citation statements)

References 17 publications

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“…Numerous studies reported the hemostatic effects of topical ABS in the animals with normal [10,12,14,[16][17][18] and defective hemostasis [15,24], and have set the preclinical stage for the development of this hemostatic product. The effects of ABS on hemostatic parameters have also been searched [2,6,25].…”

Section: Discussionmentioning

confidence: 99%

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The effects of Ankaferd Blood Stopper on transcription factors in HUVEC and erythrocyte protein profile

Yılmaz¹,

Güleç²,

Torun³

et al. 2011

Turk J Hematol

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Objective: Ankaferd ® Blood Stopper (ABS) is an herbal extract that has historically been used as a hemostatic agent in traditional Turkish medicine. ABS is comprised of a standardized herbal mixture of T. vulgaris, G. glabra, V. vinifera, A. officinarum, and U. dioica. ABS's basic mechanism of action is the formation of an encapsulated protein web, which represents the focal point for vital erythrocyte masses. The hemostatic effects of ABS have been observed in vitro and in vivo. ABS was registered as a hemostatic agent for external hemorrhages and dental bleeding following phase I randomized, double-blind crossover placebo-controlled clinical research, and safety and efficacy reports. In terms of the potential use of ABS, transcription factors may be novel factors that play a role in the hemostatic and other pleiotropic effects of ABS. Materials and Methods: Hence, the present study aimed to investigate the effects of ABS on endothelium, and possible transcription factor changes in HUVEC (human umbilical vein endothelial cells) and the erythrocyte membrane profile. ABS (5 μL and 50 μL) was administered to HUVEC (in 75 cm 2 ; ~75% fullness) for 5 min and 15 min. Results: ABS caused significant increases in the level of activation of the following transcription factors; AP2, AR, CRE/ATF1, CREB, E2F1-5, E2F6, EGR, GATA, HNF-1, ISRE, Myc-Max, NF-1, NFkB, p53, PPAR, SMAD 2/3, SP1, TRE/AP1, and YY1. Following erythrocyte membrane isolation, protein complexes were undissolved, but denatured. The protein complex formed was resistant to heat and detergent. Trypsin and sonication were used in order to break this complex; the complex dissolved and erythrocyte membrane proteins were released in SDS-PAGE. Conclusion: ABS established a very fast and solid protein web, and increased the level of transcription factor activation. Therefore the cellular effects of ABS could be related to different intracellular biological pathways. (Turk J Hematol 2011; 28: 276-85)

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Section: Discussionmentioning

confidence: 99%

“…The hemostatic effects of ABS have been observed in vitro and in vivo [12][13][14][15][16][17][18]. Use of ABS as a hemostatic agent in external hemorrhages and in dental treatment in humans provided the first data showing that ABS was safe and effective in humans [9].…”

Section: Introductionmentioning

confidence: 99%

The effects of Ankaferd Blood Stopper on transcription factors in HUVEC and erythrocyte protein profile

Yılmaz¹,

Güleç²,

Torun³

et al. 2011

Turk J Hematol

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“…Topical safety of ABS in normal healthy human volunteers was shown in a randomized double-blind cross-over Phase I clinical trial after preclinical animal studies [2][3][4][5][6]. ABS has been approved for local topical applications in external post-surgical and post-dental surgery bleedings in Turkey (www.Ankaferd.com).…”

Section: Introductionmentioning

confidence: 99%

Functional proteomic analysis of Ankaferd® Blood Stopper

Demiralp¹,

Haznedaroğlu²,

Akar³

2010

Turk J Hematol

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Objective: Ankaferd® Blood Stopper (ABS) comprises a standardized mixture of the plants Thymus vulgaris, Glycyrrhiza glabra, Vitis vinifera, Alpinia officinarum, and Urtica dioica. The basic mechanism of action for ABS is the formation of an encapsulated protein network that provides focal points for vital erythrocyte aggregation. ABSinduced protein network formation with blood cells, particularly erythrocytes, covers the primary and secondary hemostatic system without disturbing individual coagulation factors. Materials and Methods: To understand the effect mechanisms of ABS on hemostasis, a proteomic analysis using 2D gel electrophoresis and mass spectrometer was performed. Results: Proteins of plant origin in Ankaferd ® were NADP-dependent-malic enzyme, ribulose bisphosphatecarboxylase-large chain, maturase K, ATP synthase subunit-beta, ATP synthase subunit-alpha, chalcone-flavanone isomerase-1, chalcone-flavanone isomerase-2, and actin-depolymerizing factor. Furthermore, functional proteomic studies revealed that proteins resembling human peptides have been detected within Ankaferd ® , including ATP synthase, mucin-16 (CD164 sialomucin-like 2 protein), coiled-coil domain containing 141 hypothetical protein LOC283638 isoform 1, hypothetical protein LOC283638 isoform 2, dynactin 5, complex I intermediate-associated protein 30, mitochondrial, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, TP synthase, H+ transporting, mitochondrial actin binding 1 isoform, LIM domain and actin binding 1 isoform a, LIM domain and actin binding 1 isoform b, spectrin alpha non erythrocytic 1, prolactin releasing hormone receptor, utrophin, tet oncogene family member 2 isoform b, protein phosphatase 1 regulatory subunit 12A, NIMA (never in mitosis gene a)-related kinase, ATP-binding cassette protein C12, Homo sapiens malic enzyme 1, mitochondrial NADP(+)-dependent malic enzyme 3, ME2 protein, nuclear factor 1 B-type, abhydrolase domain-containing protein 12B, E3 SUMO-protein ligase PIAS2, alpha-1, 2-glucosyltransferase ALG10-A, cofilin, non-muscle isoform, 18 kDa phosphoprotein, p18, actin-depolymerizing factor (ADF), twinfilin-1, ankyrin repeat and FYVE domain-containing protein 1, usherin precursor, urotensin II receptor, interleukin 4, and midkine. Conclusion: Proteomic analysis of Ankaferd ® represents a true basis for the upcoming Ankaferd ® studies focusing on its wound healing, hemostatic, anti-infective, antineoplastic, and preservative biological actions. (Turk J Hematol 2010; 27: 70-7)

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“…The quantity of bleeding was measured as follows; rats were placed individually in a standard plastic restraining cage with the tail allowed to hang out freely, the tip of the tail of each rat was cut (4-5 mm) with a sharp pair of scissors and a stopwatch started immediately, a pre-weighed blotting paper was used to collect all drops of blood that flowed from the site of the inflicted injury, the blotting paper was re-weighed after the appearance of the last drop of blood, the difference in weight of the dry and wet blotting paper was taken as the amount of bleeding (Cipil et al, 2009;Yalcinkaya et al, 2011).…”

Section: Amount Of Bleedingmentioning

confidence: 99%

Utility of silver nanoparticles for the analysis of diosmin and rutin in Persicaria salicifolia extract, authentic and pharmaceutical dosage forms monitored with their haemostatic activity

Salem¹,

El‐Hela²,

Abdel-Hady³

2018

Afr. J. Pharm. Pharmacol.

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The present work was conducted to evaluate a simple effective technique for spectrophotometric determination of two flavonoids namely diosmin (1) and rutin (2) in the isolated fractions obtained from Persicaria salicifolia, authentic and pharmaceutical dosage forms, the method is based on the reduction of Ag + cations to silver nanoparticles (Ag-NPs) by the effect of these flavonoids in the presence of polyvinylpyrrolidone (PVP) as a stabilizing agent which result in production of intense brown colour corresponding to the formation of the surface plasmon resonance of produced silver nanoparticles. The plasmon absorbance of the Ag-NPs allows quantitative spectrophotometric determination of these flavonoids where calibration curves derived from the changes in absorbance at λ=415 and 432 nm were linear with concentration range of diosmin and rutin 1-8 and 0.5-7.0 µg/mL while their detection limits (3σ) were 0.26 and 0.12, respectively. The method was applied successfully for the determination of flavonoids in extract, authentic and pharmaceutical dosage forms. The haemostatic activity of the total methanol extract, isolated fractions, authentic and dosage forms were investigated for their usual and silver nanoparticulated forms using tail bleeding time and amount of bleeding assays in rats, where the nanoparticulated samples exhibited more significant activities.

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In Vivo Hemostatic Effect of the Medicinal Plant Extract Ankaferd Blood Stopper in Rats Pretreated With Warfarin

Abstract: ABS has in vivo hemostatic actions that may provide a therapeutic potential for the management of patients with deficient primary hemostasis in clinical medicine.

Cited by 77 publications

References 17 publications

The effects of Ankaferd Blood Stopper on transcription factors in HUVEC and erythrocyte protein profile

The effects of Ankaferd Blood Stopper on transcription factors in HUVEC and erythrocyte protein profile

Functional proteomic analysis of Ankaferd® Blood Stopper

Utility of silver nanoparticles for the analysis of diosmin and rutin in Persicaria salicifolia extract, authentic and pharmaceutical dosage forms monitored with their haemostatic activity

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