In Vivo Healthy Benefits of Galacto-Oligosaccharides from Lupinus albus (LA-GOS) in Butyrate Production through Intestinal Microbiota

Abstract

Animal digestive systems host microorganism ecosystems, including integrated bacteria, viruses, fungi, and others, that produce a variety of compounds from different substrates with healthy properties. Among these substrates, α-galacto-oligosaccharides (GOS) are considered prebiotics that promote the grow of gut microbiota with a metabolic output of Short Chain Fatty Acids (SCFAs). In this regard, we evaluated Lupinus albus GOS (LA-GOS) as a natural prebiotic using different animal models. Therefore, the aim of this work was to evaluate the effect of LA-GOS on the gut microbiota, SCFA production, and intestinal health in healthy and induced dysbiosis conditions (an ulcerative colitis (UC) model). Twenty C57BL/6 mice were randomly allocated in four groups (n = 5/group): untreated and treated non-induced animals, and two groups induced with 2% dextran sulfate sodium to UC with and without LA-GOS administration (2.5 g/kg bw). We found that the UC treated group showed a higher goblet cell number, lower disease activity index, and reduced histopathological damage in comparison to the UC untreated group. In addition, the abundance of positive bacteria to butyryl-CoA transferase in gut microbiota was significantly increased by LA-GOS treatment, in healthy conditions. We measured the SCFA production with significant differences in the butyrate concentration between treated and untreated healthy groups. Finally, the pH level in cecum feces was reduced after LA-GOS treatment. Overall, we point out the in vivo health benefits of LA-GOS administration on the preservation of the intestinal ecosystem and the promotion of SCFA production.

Lupin Protein Concentrate as a Novel Functional Food Additive That Can Reduce Colitis-Induced Inflammation and Oxidative Stress

“…Besides exerting a dose-dependent inhibition of MMP-9 in vitro [ 14 ], the LPC presents the extra advantage of being a prepared food, rather than a purified or isolated compound (nutraceutical of pharmacological), which allows it to be characterized as GRAS while permitting an easier application in functional diets [ 16 ]. As a lupin concentrate, it has the additional advantage of containing other beneficial health activities due to the presence of specific polyphenols and carbohydrates from lupin seeds which have been reported to reduce oxidative stress and improve the gut microbiome, both rather important factors in reducing or preventing IBDs [ 20 ]. Nonetheless, although LPC was tested against MMP activity [ 16 ], its nutritional profile and antioxidant activities remained to be studied.…”

“…Evidence suggests that IBDs are associated with an imbalance between ROS and antioxidant activity, which generates oxidative stress as the result of either ROS overproduction or a decrease in antioxidant activity [ 18 , 19 ], meaning that the main key players in ROS scavenging are often identified as important clinical targets in IBD management [ 17 , 18 , 19 ]. Recent reports demonstrate that lupin is a promising source of antioxidant phenolics for functional food production, as well as an inductor of a healthier gut microbiome [ 20 ]. These observations highlight the importance to further pursue the potential of this LPC as a functional ingredient, and to test its potential in vivo, both as an MMP-9 inhibitor and as an oxidative stress reducer in IBDs.…”

Dietary Resistant Starch From Potato Regulates Bone Mass by Modulating Gut Microbiota and Concomitant Short-Chain Fatty Acids Production in Meat Ducks

“…These discrepancies might interpret into that the regulation of gut microbiota on bone is not only attributed to the ratio of Firmicutes and Bacteroidetes, but also the generation of fermentation, especially SCFAs. Indeed, Firmicutes bacteria produce high amounts of butyrate and propionate, such as Faecalibacterium prausnitzii, Roseburia sp ., and Eubacterium rectale , whereas Bacteroidetes bacteria produce high levels of acetate and propionate ( 25 , 26 ). Accordingly, in the current study, the elevated content of Firmicutes resulted in a higher concentration of propionate and butyrate in cecal chyme that is consistent with our previous observations ( 14 ).…”

β-Caryophyllene: A Therapeutic Alternative for Intestinal Barrier Dysfunction Caused by Obesity

“…The quantity of goblet cells was measured using APERIO Image Scope software ( ) (accessed on: 24 March 2022) by LEICA at a 20-X magnification (100 μm). Therefore, the number of goblet cells was expressed as the “number of goblet cells per crypt per 100 µm” [ 47 ].…”