In Vivo Gene Expression Profile of Human Intestinal Epithelial Cells: From the Viewpoint of Drug Metabolism and Pharmacokinetics

Quantitative Assessment of the Impact of Crohn's Disease on Protein Abundance of Human Intestinal Drug-Metabolising Enzymes and Transporters

“…Despite the importance of the ileum in oral drug disposition, expression of transporters, such as MRP1 and MCT1, has not been reported. Some studies combined ulcerative colitis and CD patients; 18,24 others combined expression value of DMEs from the ileum and colon, 25 impeding differential analysis of the expression data. Further, the control groups were not always healthy subjects and in some studies the control group was lacking altogether 14,15,18,23,24,26 preventing the generation of disease perturbation factors (DPF).…”

“…Some studies combined ulcerative colitis and CD patients; 18,24 others combined expression value of DMEs from the ileum and colon, 25 impeding differential analysis of the expression data. Further, the control groups were not always healthy subjects and in some studies the control group was lacking altogether 14,15,18,23,24,26 preventing the generation of disease perturbation factors (DPF). The DPF reports alteration in protein expression due to disease as a ratio relative to healthy expression.…”

Analysis of Complex Absorption After Multiple Dosing: Application to the Interaction Between the P-glycoprotein Substrate Talinolol and Rifampicin

“…What remains to be explained, however, are the processes underlying the first peak (after about one hour), which is not affected by P-gp induction, and the second higher peak (between 2 and 4 h after administration), which is shifted to later times after P-gp induction. Here we offer an alternative hypothesis to the "intestinal storage model" originally suggested by Weitschies et al 2005 [15], which is based on more recent results on the abundance of multidrug transporters along the small and large intestine [23][24][25]. Intestinal P-gp is a high-affinity efflux carrier which counteracts the uptake.…”

Human duodenal organoid‐derived monolayers serve as a suitable barrier model for duodenal tissue

“…(B) Differentially expressed SLCs within duodenal tissue samples (20–116, n = 5), ODMs (21–125, n = 3), organoids (21–112; n = 8), and Caco‐2 (27–115; n = 6). Each symbol presents one dataset: gray square ■ = GSE156453, 39 white square □ = Ma'ayeh et al. ; 44 black circle ● = GSE164334, 38 black triangle ▲ = GSE160695, 43 white circle ○ = GSE163706, 42 black square ◆ = GSE127938, 40 gray circle = GSE167286, 41 black star ★ = own data 15 (values are shown as mean ± SEM)…”

“…Comparison of the qualitative expression of typical transporter genes between Caco‐2, tissue, ODMs, and organoids was performed using the following data sets, available at Gene Expression Omnibus (GEO): GSE164334, 38 GSE156453, 39 GSE127938, 40 GSE167286, 41 GSE163706, and 42 GSE160695; 43 and one data set from Ma'ayeh et al. 44 and our own data 15 .…”