In vivo fluorescence imaging of threadlike tissues (Bonghan ducts) inside lymphatic vessels with nanoparticles

Yoo, Jung Sun; Johng, Hyeon-Min; Yoon, Tae‐Jong; Shin, Hyun-Chool; Lee, Byung-Cheon; Lee, Chang‐Hoon; Ahn, Byung Soo; Kang, Dae-In; Lee, Jin-Kyu; Soh, Kwang–Sup

doi:10.1016/j.cap.2006.09.011

Cited by 30 publications

(14 citation statements)

References 20 publications

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“…They are parts of a circulatory system that is thought to be the anatomical basis of classical acupuncture meridians and consists of several subsystems: superficial BHDs (Kim et al 2006) located in the skin, intravascular BHDs which are inside large blood vessels and lymphatic vessels, and organ surface BHDs on the surface of various internal organs. The intravascular BHDs inside blood vessels were confirmed using an acridine-orange staining method (Lee et al 2004a;Baik et al 2005), and those inside lymphatic vessels were recently observed employing a Janus Green B staining technique (Lee et al 2005a) as well as a nanoparticle injection method Yoo et al 2007). …”

Section: Introductionmentioning

confidence: 99%

“…A series of investigations have been performed to establish the novelty of the NTSs and to elucidate their details, looking into: (1) the anatomy and the basic histology using conventional staining methods and confocal laser scanning microscopy (Lee et al 2004a(Lee et al , 2005aShin et al 2005); (2) the ultrastructure using various electron microscopy such as scanning electron microscopy (SEM), transmission electron microscopy (TEM), cryo-SEM, and SEM with a focused-ion-beam (FIB/SEM; Lee et al 2007;Yoo et al 2007); (3) the cellular nature by using immunohistochemistry ; and (4) the circulatory function by injecting nanoparticles. The present work is concerned with the last category of these investigations.…”

Section: Introductionmentioning

confidence: 99%

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Measurement of flow speed in the channels of novel threadlike structures on the surfaces of mammalian organs

Sung

Kim

Lee

et al. 2007

Naturwissenschaften

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There have been several reports on novel threadlike structures (NTSs) on the surfaces of the internal organs of rats and rabbits since their first observation by Bonghan Kim in 1963. To confirm this novel circulatory function, it is necessary to observe the flow of liquid through the NTS as well as the structurally corroborating channels in the NTS. In this article, we report on the measurement of the flow speed of Alcian blue solution in the NTSs on the organ surfaces of rabbits, and we present electron microscopic images depicting the cribrous cross-section with channels. The speed was measured as 0.3 +/- 0.1 mm/s, and the flow distance was up to 12 cm. The flow was unidirectional, and the phase contrast microscopic images showed that the NTSs were strongly stained with Alcian blue. The ultrastructure of the NTSs revealed by cryo-scanning electron microscopy and high-voltage electron microscopy showed that (1) there were cell-like bodies and globular clumps of matter inside the sinus of the channel with thin strands of segregated zones which is a microscopic evidence of the liquid flow, (2) the sinuses have wall structures surrounded with extracellular matrices of collagenous fibers, and (3) there exists a cribriform structure of sinuses. To understand the mechanism for the circulation, a quantitative analysis of the flow speed has been undertaken applying a simplified windkessel model. In this analysis, it was shown that the liquid flow through the NTSs could be due to peristaltic motion of the NTS itself.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Measurement of flow speed in the channels of novel threadlike structures on the surfaces of mammalian organs

Sung

Kim

Lee

et al. 2007

Naturwissenschaften

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“…In this regard, in vivo fluorescence imaging method of novel threadlike tissues (Bonghan ducts) inside the lymphatic vessels of rats with fluorescent magnetic nanoparticles has been investigated (Yoo et al, 2007). The results of this study showed new applications of nanoparticles for in vivo imaging of hardly detectable tissues using fluorescence reflectance imaging and magnetophoretic control.…”

Section: In Vivo Fluorescence Spectroscopymentioning

confidence: 90%

“…Magnetic nanoparticles have been widely used in biomedical research such as magnetic carriers for bioseparation (Dolye et al, 2002, as cited in Yoo at al., 2007, enzyme and protein immobilization (Cao et al, 2003, as cited in Yoo at al., 2007, and contrast-enhancing media (Wu et al, as cited in Yoo at al., 2007). In this regard, in vivo fluorescence imaging method of novel threadlike tissues (Bonghan ducts) inside the lymphatic vessels of rats with fluorescent magnetic nanoparticles has been investigated (Yoo et al, 2007).…”

Section: In Vivo Fluorescence Spectroscopymentioning

confidence: 99%

Current Achievement and Future Potential of Fluorescence Spectroscopy

Al-Rawashdeh¹

2012

Macro to Nano Spectroscopy

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“…23 A sonicator (Digital Sonifier 450, Branson Co., Danbury, Connecticut) was used for breaking aggregates of nanoparticles in the suspension. Gray-scale images were analyzed using IMAGEJ software (Wayne Rasband, National Institute of Health, v1.43u).…”

Section: Imaging Device and Image Processingmentioning

confidence: 99%

Estimating the density of fluorescent nanoparticles in the primo vessels in the fourth ventricle and the spinal cord of a rat

et al. 2011

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Abstract. The primo vascular system is a novel circulatory system forming a network throughout an animal's body. Primo vessels were recently observed in the fourth ventricle of the brain and in the spinal cord of a rat by using fluorescent nanoparticles. In order to quantify the nanoparticles in the primo vessels, we measured the florescence of the nanoparticles and calibrated the measurements by using a reference suspension. We removed the noise due to autofluorescence with the technique of multispectral imaging. The line densities of nanoparticles and the contrast values of their images were, respectively, 0.5 ± 0.5 ng/mm and 0.7 ± 0.5 for primo vessels in the fourth ventricle, and 1.3 ± 0.6 ng/mm and 1.4 ± 0.2 for primo vessels in the spinal cord. The data obtained from and the procedures used in this work could be useful in evaluating the feasibility of using nanoparticles as a contrast agent during MRI or CT imaging of primo vessels in the brain or the spinal cord. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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In vivo fluorescence imaging of threadlike tissues (Bonghan ducts) inside lymphatic vessels with nanoparticles

Cited by 30 publications

References 20 publications

Measurement of flow speed in the channels of novel threadlike structures on the surfaces of mammalian organs

Measurement of flow speed in the channels of novel threadlike structures on the surfaces of mammalian organs

Current Achievement and Future Potential of Fluorescence Spectroscopy

Estimating the density of fluorescent nanoparticles in the primo vessels in the fourth ventricle and the spinal cord of a rat

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