In-vivo fluorescence imaging of adenosine 5′-triphosphate

Dong, Jiantong; Zhao, Meiping

doi:10.1016/j.trac.2016.03.020

Cited by 58 publications

(33 citation statements)

References 98 publications

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“…The recent development of ATP-biosensors enabled us to monitor changes in ATP concentration in single living cells (reviewed in Dong and Zhao, 2016). ATeam, the first FRET-based ATP biosensor, has successfully been introduced into mammalian cells and is now widely used to visualize ATP dynamics in living cells (Imamura et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Reliable imaging of ATP in living budding and fission yeast

Takaine

Ueno

Kitamura

et al. 2019

Journal of Cell Science

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Adenosine triphosphate (ATP) is a main metabolite essential for all living organisms. However, our understanding of ATP dynamics within a single living cell is very limited. Here, we optimized the ATP-biosensor QUEEN and monitored the dynamics of ATP with good spatial and temporal resolution in living yeasts. We found stable maintenance of ATP concentration in wild-type yeasts, regardless of carbon sources or cell cycle stages, suggesting that mechanism exists to maintain ATP at a specific concentration. We further found that ATP concentration is not necessarily an indicator of metabolic activity, as there is no clear correlation between ATP level and growth rates. During fission yeast meiosis, we found a reduction in ATP levels, suggesting that ATP homeostasis is controlled by differentiation. The use of QUEEN in yeasts offers an easy and reliable assay for ATP dynamicity and will answer several unaddressed questions about cellular metabolism in eukaryotes.

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Section: Introductionmentioning

confidence: 99%

Reliable imaging of ATP in living budding and fission yeast

Takaine

Ueno

Kitamura

et al. 2019

Journal of Cell Science

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“…Despite these advances, however, a few aptamer‐based diagnostic or therapeutic products are on the market. From the perspective of in vivo applications, aptamers without specific modification are negatively charged and naturally repelled from the cell membrane that carries the same charge, hence, limiting their ability to cross the cell membrane . Moreover, aptamers without protecting modifications are susceptible to degradation by nucleases inside the cellular matrix.…”

Section: Molecular Engineering Of Aptamer‐based Nanomaterials Toward mentioning

confidence: 99%

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Zhang

Lan

2019

Adv Healthcare Materials

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equally exciting and perhaps more challenging field of application is toward their in vivo applications, including living animals and human bodies, in diverse areas, [2] such as sensing, drug delivery, medical imaging, and cancer therapy. However, most of these nanomaterials lack selectivity toward targets of interests. Therefore, to employ these nanomaterials for a wider range of in vivo applications, various molecular engineering approaches have been employed to obtained nanomaterials functionalized with biomolecules to enable selective targeting. [3][4][5] Among these biomolecules, functional nucleic acids, or FNAs, have shown the most promise.FNAs are DNA or RNA molecules that can interact with or bind to a specific analyte, resulting in a conformational change and/or a catalytic reaction. [6] As their names suggested, ribozymes and deoxyribozymes (called DNAzyme hereafter) can act as enzymes in the presence of cofactors to catalyze various reactions, including cleavage and ligation of RNA/DNA, and phosphorylation and peroxidation of DNA. On the other hand, riboswitches and DNA aptamers are antibody-like receptors that can bind target molecules with high affinity and selectivity. Furthermore, the binding abilities of riboswitches and aptamers can be combined with the enzymatic function of ribozymes or DNAzymes to form aptazymes so that the binding of targets can inhibit or enhance the catalytic activities. These nucleic acids, including DNAzymes, aptamers, and aptazymes, are collectively known as FNAs to emphasize their functions beyond the traditional genetic storage and transformations roles for DNA and RNA. Unlike DNA and RNA inside biological systems, FNAs are isolated via a combinatorial technique known as in vitro selection, or a process also known as systematic evolution of ligands by exponential enrichment (SELEX). The discovery of new functions of nucleic acids has not only resulted in major advances in the fields of molecular biology and biochemistry, but also revolutionized sensors designs for both in vitro and in vivo applications. [7,8] Over the past decade, numerous FNA-based nanomaterials have been developed. [9][10][11][12] Among these FNA-based nanosystems, the functions of FNAs can be categorized into two types: diagnostic function and therapeutic function. For the diagnostic function, the FNAs serve either as the biorecognition ligands for direct sensing and imaging of the Recent advances in nanotechnology and engineering have generated many nanomaterials with unique physical and chemical properties. Over the past decade, numerous nanomaterials are introduced into many research areas, such as sensors for environmental monitoring, food safety, point-ofcare diagnostics, and as transducers for solar energy transfer. Meanwhile, functional nucleic acids (FNAs), including nucleic acid enzymes, aptamers, and aptazymes, have attracted major attention from the biomedical community due to their unique target recognition and catalytic properties. Benefiting from the recent progress of molecular engine...

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“…Since the nucleotides, especially ATP is pivotal in living systems [38],numerous fluorescent chemosensors have been developed for their detection [39][40][41][42][43], involving direct sensing [44,45], cation-based recognition units [46][47][48][49][50][51][52] and also some indicator displacement assays [53][54][55][56][57].…”

Section: Introductionmentioning

confidence: 99%