In Vivo FF-SS-OCT Optical Imaging of Physiological Responses to Photostimulation of Human Photoreceptor Cells

Hillmann, Dierck; Pfäffle, Clara; Spahr, Hendrik; Sudkamp, Helge; Franke, Gesa; Hüttmann, Gereon

doi:10.1007/978-3-030-16638-0_8

Cited by 3 publications

(5 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Adaptive optic technology has enabled the observation of the spatial distribution of photoreceptor cells or photoreceptor inner segments [ 48 ], and adaptive optical coherence tomography can now visualize individual cone photoreceptor cells and Müller cells [ 49 ]. Phase-sensitive full-field swept-source optical coherence tomography (FF-SS-OCT) can capture osmotic effects on the outer segment of the eye, thereby allowing the observation of single cone activity in the human eye [ 50 ]. In addition, by using FF-SS-OCT on the eye of a healthy volunteer, simultaneous functional imaging of activation in the photoreceptor and ganglion cell layer/inner plexiform layer was acquired [ 51 ].…”

Section: Future Directionsmentioning

confidence: 99%

Pluripotent stem cell-derived retinal organoid/cells for retinal regeneration therapies: A review

Mandai¹

2023

Regenerative Therapy

View full text Add to dashboard Cite

Section: Future Directionsmentioning

confidence: 99%

Pluripotent stem cell-derived retinal organoid/cells for retinal regeneration therapies: A review

Mandai¹

2023

Regenerative Therapy

View full text Add to dashboard Cite

“…A hybrid line-scan SLO and OCT system was also employed for confocal-OCT IOS imaging study of isolated retinas ( 47 ). Given the improved imaging speed, Fourier-domain OCT has dominated recent IOS imaging studies of both animal ( 33 , 35 , 37 , 39 , 42 , 44 , 48 – 52 ) and human ( 25 , 31 , 32 , 36 , 37 , 53 – 55 ) retinas.…”

Section: Functional Oct Developments For Intrinsic Signal Orgmentioning

confidence: 99%

“…High-speed 3D imaging can reduce intraframe eye movement artifacts, which permits robust registration of frames and tracking of photoreceptors, returning stable OCT phase information. Access to stable phase information allows detecting cellular deformations much smaller than its axial resolution, and the phase information has been recently used to measure light-evoked photoreceptor outer segment (OS) deformation ( 32 , 36 , 55 , 58 ). However, the parallel OCT suffers resolution loss from multiple scattering crosstalk.…”

Section: Functional Oct Developments For Intrinsic Signal Orgmentioning

confidence: 99%

See 1 more Smart Citation

Functional Optical Coherence Tomography for Intrinsic Signal Optoretinography: Recent Developments and Deployment Challenges

Kim

Son

et al. 2022

Front. Med.

View full text Add to dashboard Cite

Intrinsic optical signal (IOS) imaging of the retina, also termed as optoretinogram or optoretinography (ORG), promises a non-invasive method for the objective assessment of retinal function. By providing the unparalleled capability to differentiate individual retinal layers, functional optical coherence tomography (OCT) has been actively investigated for intrinsic signal ORG measurements. However, clinical deployment of functional OCT for quantitative ORG is still challenging due to the lack of a standardized imaging protocol and the complication of IOS sources and mechanisms. This article aims to summarize recent developments of functional OCT for ORG measurement, OCT intensity- and phase-based IOS processing. Technical challenges and perspectives of quantitative IOS analysis and ORG interpretations are discussed.

show abstract

“…Since the early days, in parallel to the spectral domain (Fourier domain) OCT, another class of en face OCT methods have been under development to increase en face imaging speed. Advances in camera technologies and light sources in recent years enabled the rise of en face OCT methods, such as Fourier-domain full-field OCT (FD-FF-OCT), [23][24][25][26] also known as swept-source full-field OCT (SS-FF-OCT), [27][28][29][30] line-field OCT (LF-OCT), [31][32][33][34][35] ultra-high-resolution OCT (UHR-OCT), [36][37][38][39][40][41][42] and so on. An excellent review of en face OCT methods can be found.…”

mentioning

confidence: 99%

Time-domain full-field optical coherence tomography (TD-FF-OCT) in ophthalmic imaging

Zhang

Mazlin

Fei

et al. 2023

Therapeutic Advances in Chronic Disease

View full text Add to dashboard Cite

Ocular imaging plays an irreplaceable role in the evaluation of eye diseases. Developing cellular-resolution ophthalmic imaging technique for more accurate and effective diagnosis and pathogenesis analysis of ocular diseases is a hot topic in the cross-cutting areas of ophthalmology and imaging. Currently, ocular imaging with traditional optical coherence tomography (OCT) is limited in lateral resolution and thus can hardly resolve cellular structures. Conventional OCT technology obtains ultra-high resolution at the expense of a certain imaging range and cannot achieve full field of view imaging. In the early years, Time-domain full-field OCT (TD-FF-OCT) has been mainly used for ex vivo ophthalmic tissue studies, limited by the low speed and low full-well capacity of existing two-dimensional (2D) cameras. The recent improvements in system design opened new imaging possibilities for in vivo applications thanks to its distinctive optical properties of TD-FF-OCT such as a spatial resolution almost insensitive to aberrations, and the possibility to control the curvature of the optical slice. This review also attempts to look at the future directions of TD-FF-OCT evolution, for example, the potential transfer of the functional-imaging dynamic TD-FF-OCT from the ex vivo into in vivo use and its expected benefit in basic and clinical ophthalmic research. Through non-invasive, wide-field, and cellular-resolution imaging, TD-FF-OCT has great potential to be the next-generation imaging modality to improve our understanding of human eye physiology and pathology.

show abstract

In Vivo FF-SS-OCT Optical Imaging of Physiological Responses to Photostimulation of Human Photoreceptor Cells

Cited by 3 publications

References 55 publications

Pluripotent stem cell-derived retinal organoid/cells for retinal regeneration therapies: A review

Pluripotent stem cell-derived retinal organoid/cells for retinal regeneration therapies: A review

Functional Optical Coherence Tomography for Intrinsic Signal Optoretinography: Recent Developments and Deployment Challenges

Time-domain full-field optical coherence tomography (TD-FF-OCT) in ophthalmic imaging

Contact Info

Product

Resources

About