In Vivo Expression of Neurotrophins and Neurotrophin Receptors Is Conserved in Adult Porcine Retina In Vitro

Garcı́a, Mónica; Forster, Valérie; Hicks, David; Vecino, Elena

doi:10.1167/iovs.03-0419

Cited by 59 publications

(49 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Immunolocalization of the TrkA revealed positive staining of retinal pigment epithelium and Müller cells. This result is consistent with previous studies [10,11,12,13,14,15]. …”

Section: Discussionsupporting

confidence: 94%

Nerve Growth Factor Helps Protect Retina in Experimental Retinal Detachment

Sun

Xu²,

Wang³

et al. 2007

Ophthalmologica

View full text Add to dashboard Cite

Purpose: To investigate the expression of nerve growth factor (NGF) and its receptor TrkA on the retina at different time points after retinal detachment (RD) and the protection effect of NGF in experimental RD. Methods: Sprague-Dawley rats were used as an RD animal model by injection of 0.1% sodium hyaluronate under the neurosensory retina. The expression of endogenous NGF and its receptor TrkA in the rat retina was detected using immunohistochemistry. The NGF (5 µg/eye) or phosphate-buffered saline were injected separately into vitreous every 4 days after the RD. The rat eyes were then observed at various time points. Morphologic changes were investigated by light microscopy. Retinal gliosis was detected by glial fibrillary acidic protein labeling. Results: The expression of endogenous NGF and TrkA was upregulated during RD procedure. Most of the NGF-treated retina had a well-organized structure. In NGF-treated RD eyes, the cells were still significantly more numerous than in phosphate-buffered-saline-treated retina. Glial fibrillary acidic protein labeling increased quickly after RD; the NGF-treated retina had less reactive gliosis than the control groups. Conclusions: Intravitreal injection of exogenous NGF can protect retinal cells from degeneration in experimental RD. It may exert its protective action by preventing the apoptosis of retinal cells after RD.

show abstract

“…Immunolocalization of the TrkA revealed positive staining of retinal pigment epithelium and Müller cells. This result is consistent with previous studies [10,11,12,13,14,15]. …”

Section: Discussionsupporting

confidence: 94%

Nerve Growth Factor Helps Protect Retina in Experimental Retinal Detachment

Sun

Xu²,

Wang³

et al. 2007

Ophthalmologica

View full text Add to dashboard Cite

show abstract

“…Moreover, the expression of different subtypes of neurofilaments among RGCs was found to be similar, irrespective of soma size. Nevertheless, we did find differential expression of other molecules, such as neurotrophin-3, in different types of porcine RGCs (García et al, 2003).…”

Section: Rgc Soma Sizescontrasting

confidence: 55%

Topography of pig retinal ganglion cells

Garcá

Ruiz-Ederra

Hernández-Barbáchano

et al. 2005

J of Comparative Neurology

Self Cite

View full text Add to dashboard Cite

In the present work we analyzed the distribution of retinal ganglion cells (RGCs) in the pig retina. RGCs were retrogradely labeled in vivo by injecting Fluoro-Gold into the optic nerve. RGC density and the distribution of RGCs in terms of soma size were analyzed. Different regions of the porcine retina were identified following analysis of the distribution of RGCs in terms of cell density and soma size: in the central retina, we found a high-density horizontal RGC band lying dorsal to the optic disc. Moreover, in this region, a high proportion of RCGs with small soma size was observed. From the central to the more peripheral retina, we observed a decrease in RGC density, together with a greater presence of RGCs with larger somas. The results of this study should prove to be useful as a foundation for future studies with the porcine retina as a model in ophthalmic research. The study also highlights the necessity to label the RGC population specifically with retrograde tracers in order to quantify precisely alterations in the cell population associated with experimental treatments.

show abstract

“…The temporal and spatial expression patterns of BDNF and TrkB receptors within the developing visual system indicate that BDNF is available to influence important aspects of RGC differentiation, including their morphological maturation. BDNF and its receptor TrkB are highly expressed in the visual system of most vertebrate species examined, from fish to mammals (Cellerino and Kohler, 1997;Cohen-Cory et al, 1996;Cohen-Cory and Fraser, 1994;Duprey-Diaz et al, 2002;Frost et al, 2001;Garcia et al, 2003;Hallbook et al, 1996;Hashimoto and Heinrich, 1997;Herzog et al, 1994;Herzog and von Bartheld, 1998;Jelsma et al, 1993;Perez and Caminos, 1995). BDNF was initially characterized for its ability to promote survival of cultured RGCs (Cohen-Cory and Fraser, 1994;Johnson et al, 1986;Rodriguez-Tebar et al, 1989), but BDNF does not modulate RGC programmed cell death in vivo because RGC numbers in TrkB-or BDNF-deficient mice are similar to wild types Pollock et al, 2003;Rohrer et al, 2001).…”

Section: S Cohen-cory and B Lommentioning

confidence: 99%

Neurotrophic regulation of retinal ganglion cell synaptic connectivity: from axons and dendrites to synapses

Cohen-Cory

Lom²

2004

Int. J. Dev. Biol.

View full text Add to dashboard Cite

This review highlights important events during the morphological development of retinal ganglion cells (RGCs), focusing on mechanisms that control axon and dendritic arborization as a means to understand synaptic connectivity with special emphasis on the role of neurotrophins during structural and functional development of RGCs. Neurotrophins and their receptors participate in the development of visual connectivity at multiple levels. In the visual system, neurotrophins have been shown to exert various developmental influences, from guiding the morphological differentiation of neurons to controlling the functional plasticity of visual circuits. This review article examines the role of neurotrophins, and in particular of BDNF, during the morphological development of RGCs, and discusses potential interactions between activity and neurotrophins during development of neuronal connectivity. Retinal ganglion cells (RGCs), the sole projection neurons from the retina, integrate, process, and convey all visual information transmitted to the brain (Dowling, 1987). Within the retina, RGCs attain their fates and differentiate early, preceding other types of retinal neurons (Fig. 1). RGCs reside near the lens in the ganglion cell layer (GCL) and exhibit distinctive, characteristic morphologies. Different morphological subtypes of mature RGCs are characterized by soma size and dendritic arborization patterns. To receive synaptic inputs from amacrine and bipolar neurons, each RGC elaborates several branched dendrites into the retina's inner plexiform layer (IPL) and to provide synaptic input to the central targets, each RGC extends a single axon that exits the retina via the optic nerve to synapse on neurons in the brain (the optic tectum in lower vertebrates or superior colliculus, pretectum, and lateral geniculate nucleus in higher vertebrates). When the RGC growth cone has reached and recognized its target region, the growth cone transforms into an actively branching axon terminal, extending and retracting branches it forms synapses with target neurons in the brain. The events of RGC axon extension, growth cone pathfinding, and target recognition all coincide with RGC dendritic arborization in the retina. RGCs begin to extend primary dendrites as their axons course towards their targets, and continue to elaborate complex dendritic arbors as their axon terminals innervate and branch within the target (Holt, 1989). KEY WORDS: retina, optic tectum, arborization, BDNF, visual systemThis review highlights important events during the morphological development of RGC that influence both the presynaptic (dendritic) and postsynaptic (axon) connectivity of RGC projection neurons. The elucidation of the events that guide development and differentiation of RGCs is due in part to recent advances in in vivo imaging techniques that have permitted visualization of cellular events that underlie the establishment of RGC synaptic connectivity (Cohen-Cory, 2002;Debski and Cline, 2002). The frog and fish retinotectal projections have p...

show abstract

In Vivo Expression of Neurotrophins and Neurotrophin Receptors Is Conserved in Adult Porcine Retina In Vitro

Cited by 59 publications

References 56 publications

Nerve Growth Factor Helps Protect Retina in Experimental Retinal Detachment

Nerve Growth Factor Helps Protect Retina in Experimental Retinal Detachment

Topography of pig retinal ganglion cells

Neurotrophic regulation of retinal ganglion cell synaptic connectivity: from axons and dendrites to synapses

Contact Info

Product

Resources

About