In Vivo Examinations on the Biocompatibility of Poly(d,l)lactide

Heidemann, W.; Ruffieux, Kurt; Jeschkeit-Schubbert, S.; J, Fischer; Wintermantel, E.; Jung, Hee Kyeong; Krüger, Gerhard; Gerlach, Klaus

doi:10.1515/bmte.2001.46.s1.206

Cited by 3 publications

(9 citation statements)

References 2 publications

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“…At regulär time intervals (2,4,6,8,12,16,20,24,28,32,36,40,44, 52 weeks after Implantation) three randomly sampled rats were narcotized VNith ether/O 2 . The pH values of the normal muscles, at least 2 cm distant from the implants, äs well äs the pH values of the tissue sunroun?…”

Section: Determinationofphvaluementioning

confidence: 99%

“…The biocompatibility of intrinsically amorphous Poly(D,L)lactide (PDLIj\) implants was demonstrated in vivo; the polymer was macroscopically resorbed within 52 weeks and even microscopically detectable remnants were resorbed from die extracellular space within 72 weeks post Implantation [9]. It has also been shown that a decrease in pH value occurs in both in vitro incubation solutions containing samples of pure PDLLA [10,11], and in vivo tissues in the vicinity of pre-degraded PDLLA [12].…”

Section: Introductionmentioning

confidence: 99%

“…The admixture of sodium hydrogen phosphate (NaP) to PDLLA led to a certain stabilization of pH value in the environment of samples in vitro and [11,12]. The admixture of sodium hydrogen phosphate (NaP) to PDLLA led to a certain stabilization of pH value in the environment of samples in vitro and [11,12].…”

Section: Introductionmentioning

confidence: 99%

“…In a previous study it was suggested that a fibrous tissue layer surrounding the implants might act äs a barrier, thus impeding the removal of degradation products [9]. Additionally, it was to lest if the admixturc of a small quantity of NaP particles would significantly change the mechanical properties of PDLLA.…”

Section: Introductionmentioning

confidence: 99%

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The Effect of an Admixture of Sodium Hydrogen Phosphate or Heparin-coating to Poly(D,L)lactide - Results of an Animal Study. In-vivo-Studie über die Auswirkung des Zusatzes von Natriumhydrogenphosphat zu Poly(D,L)laktid und über die Oberflächenbeschichtung von Poly(D,L)laktid mit Heparin

Heidemann¹,

Ruffieux²,

Fischer³

et al. 2003

Biomedizinische Technik/Biomedical Engineering

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The study was aimed at investigating the effect of an admixture of sodium hydrogen phosphate (NaP) on the pH value around degrading poly(D,giactide (PDLLA) and the possible improvement of PDLLA biocompatibility by coating its surface with heparin. PDLLA ± NaP was injection-molded to form rods (20 3 2 (mm)) and cubes (3x2x2 (mm)). Half of the pure PDLLA samples were surface-coated using heparin. One rod and cube each of PDLLA, PDL-LA+NaP and PDLLA/Hep were implanted into the dorsal muscles of 42 rats. From the Z* to 52 nd week after Operation, pH measurements were performed in the environment around the implants. The samples were then harvested for histological and mechanical analyses. No significant decrease in pH-values was observed in the tissue around the implants. Pure PDLLA and PDLLA/Hep samples were macroscopically resorbed after 52 weeks, while the degradation of PDLLA+NaP was still in progress. Approximately 80% of the initial bending strength of PDLLA or PDLLA/Hep rods was present after six weeks, while the bending strength of PDLLA+Nap was reduced to 50% after 4 weeks. Heparin-coating of PDLLA did not improve its btocompatibility but did increase its resorption. While no significant effect of NaP on pH value was found, its admixture did reduce the mechanical characteristics of the implants.Schlüsselwörter: Poly(D,L)Laktid, PDLLA, Natriumhydrogenphosphat, Heparin-Beschichtung in der vorliegenden Studie wurde untersucht, ob durch eine Beimengung von Natriumhydrogenphosphat (NaP) zu Poly(D,L)Laktid (PDL-LA) der Gewebe-pH-Wert während des Abbaus stabilisiert wird und inwieweit durch eine Oberflächenbeschichtung mit Heparin die Biokompatibilität von PDLLA verbessert werden kann. Stäbchen (20 3 2 (mm)) und Würfel (3x2x2 (mm)) aus PDLLA mit und ohne Beimengung von NaP wurden spritzgegossen, und die Hälfte der Proben aus reinem PDLLA wurde mit Heparin beschichtet. Je ein Stäbchen und ein Würfel aus PDLLA, PDLLA + NaP und PDL-LA/Heparin wurden in die Rückenmuskulatur von 42 Ratten implantiert. Zwischen der 2. und der 52. Woche nach der Implantation wurden die Proben nach Messung der pH-Werte entnommen und histologisch sowie biomechanisch untersucht. Ein signifikanter Abfall der pH-Werte im Gewebe in der Umgebung der Implantate trat während der Degradation nicht auf. Die PDLLA-und PDLLA/Hep-Proben waren nach 52 Wochen makroskopisch resorbiert, die Degradation der PDLLA+NaP-lmplantate war zu diesem Zeitpunkt noch nicht abgeschlossen. Die PDLLA-und PDLLA/Hep-Stäbchen wiesen nach 6 Wochen noch 80% ihrer Anfangsbiegefestigkeit auf, die Biegefestigkeit der PDLLA+NaP-Stäbchen war bereits nach 4 Wochen auf 50 % gefallen. Durch die Heparin-Beschichtung konnte keine Verbesserung der Biokompatibilität von PDLLA nachgewiesen werden, dagegen verlängerte sich die Resorptionszeit. Eine Beimengung von NaP zu PDLLA führte nicht zu einer pH-Stabilisierung, verschlechterte aber die mechanischen Eigenschaften der Implantate.

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Section: Determinationofphvaluementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Effect of an Admixture of Sodium Hydrogen Phosphate or Heparin-coating to Poly(D,L)lactide - Results of an Animal Study. In-vivo-Studie über die Auswirkung des Zusatzes von Natriumhydrogenphosphat zu Poly(D,L)laktid und über die Oberflächenbeschichtung von Poly(D,L)laktid mit Heparin

Heidemann¹,

Ruffieux²,

Fischer³

et al. 2003

Biomedizinische Technik/Biomedical Engineering

Self Cite

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“…К его недостаткам можно отнести риск закисления тканей в про-цессе деградации [2,7]. Полигидроксиалканоа-ты -линейные полимеры, получаемые микробио-логическим путем, при этом в силу особенностей биосинтеза, характеризуются широким спектром значений скорости деградации и механических параметров, что позволяет применять полиокси-алканоаты в различных областях медицинской науки и практики [8,13,21].…”

Section: Introductionunclassified

Influence of Composition and Concentration of Biodegradable Polymers on Structure and Physico-Mechanical Properties of Electrospun Scaffolds

Шишкова¹,

Насонова²,

Ходыревская³

et al. 2016

Kompleks. probl. serdečno-sosud. zabol.

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Результаты. Концентрация раствора полимера значительно влияет на диаметр волокон, формируемых в процессе ЭС, при этом не оказывая существенного влияния на размер образуемых пор. Использование 6 % раствора полимеров не позволяет полу-чать полноценные волокна, что негативно отражается на их морфологии и физико-механических свойствах. для создания матриц на основе ПгБВ, ПлА и ПКл оптимальнее всего использовать концентрацию раствора 8-10 %. добавление к ПгБВ ПлА уменьшает диаметр волокон и размер пор, а включение в полимерную композицию ПлА приводит к увеличению эластичности матриц.Заключение. Состав полимерной композиции и концентрация раствора оказывают существенное влияние на размер пор, структуру и диаметр волокон, формируемых в процессе электроспиннинга, что в свою очередь сказывается на физико-механиче-ских свойствах получаемых матриц.Ключевые слова: матрицы, биодеградируемые полимеры, электроспиннинг, пористость. Purpose. to investigate the composition and concentration of the polymer solutions on structure and physico-mechanical properties of the electrospun scaffolds. INFLUENCE OF COMPOSITION AND CONCENTRATION OF BIODEGRADABLE POLYMERS ON STRUCTURE AND PHYSICO-MECHANICAL PROPERTIES OF ELECTROSPUN SCAFFOLDSMaterials and methods. We prepared electrospun scaffolds from poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), polycaprolactone (PCL), and poly(d,L-lactide) (PLA) in concentrations of 6, 8, and 10 %. We investigated mechanical properties of the scaffolds, fiber diameter, and pore size.

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The influence of biocomposites containing genetically modified flax fibers on gene expression in rat skeletal muscle

Gredes¹,

Kunert‐Keil²,

Dominiak³

et al. 2010

Biomedizinische Technik/Biomedical Engineering

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In many studies, natural flax fibers have been proven to be resistant and surgically suitable. Genetically modified flax fibers, derived from transgenic flax expressing three bacterial genes for the synthesis of poly-3-hydroxybutyric acid (PHB), have better mechanical properties than unmodified flax fibers. The aim of this study was to examine the biocompatibility of composites containing flax fibers from transgenic polyhydroxybutyrate producing (M50) and control (wt-NIKE) plants in a polylactide (PLA) matrix in rat Musculus latissimus dorsi. For this purpose, effects of biocomposites on the expression of growth factors and osteogenic differentiation, in particular the mRNA expression of vascular endothelial growth factor, insulin like growth factor 1, insulin like growth factor 2, collagen-1, collagen-2 and myostatin, were analyzed using quantitative RT-PCR. The biocomposites did not show any inflammation response after subcutaneous insertion. The results following subcutaneous insertion of PLA alone and PLA-M50 showed no significant changes on the gene expression of all tested genes, whereas PLA-wt-NIKE reduced the mRNA amount of myostatin, VEGFA and IGF2, respectively. It can be asserted that modified flax membranes with PHB and other organic substances have a good biocompatibility to the muscle and they do not disrupt the muscle function. Furthermore, composites from transgenic flax plants producing PHB did not differ from composites of non-transgenic flax plants.

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In Vivo Examinations on the Biocompatibility of Poly(d,l)lactide

Cited by 3 publications

References 2 publications

The Effect of an Admixture of Sodium Hydrogen Phosphate or Heparin-coating to Poly(D,L)lactide - Results of an Animal Study. In-vivo-Studie über die Auswirkung des Zusatzes von Natriumhydrogenphosphat zu Poly(D,L)laktid und über die Oberflächenbeschichtung von Poly(D,L)laktid mit Heparin

The Effect of an Admixture of Sodium Hydrogen Phosphate or Heparin-coating to Poly(D,L)lactide - Results of an Animal Study. In-vivo-Studie über die Auswirkung des Zusatzes von Natriumhydrogenphosphat zu Poly(D,L)laktid und über die Oberflächenbeschichtung von Poly(D,L)laktid mit Heparin

Influence of Composition and Concentration of Biodegradable Polymers on Structure and Physico-Mechanical Properties of Electrospun Scaffolds

The influence of biocomposites containing genetically modified flax fibers on gene expression in rat skeletal muscle

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