In vivo evaluation of vaginal films for mucosal delivery of nitric oxide

Yoo, Jin‐Wook; Acharya, Gayathri; Lee, Chi H.

doi:10.1016/j.biomaterials.2009.04.004

Cited by 50 publications

(40 citation statements)

References 39 publications

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“…For experimental details, see the Electronic Supplementary Material (ESM) [8,9]. Under an Bapparent electroosmotic mobility^(including suction effect) of 77×10 −5 cm 2 V −1 s −1 , the electrophoresis of a six-month-aged GSNO sample (stored in the solid state in the freezer) solubilized in BGE just before analysis gives rise to a UV electropherogram with two well-resolved peaks (labelled A and B) and a third, wider peak that seems, on referring to the MS electropherogram (in total ionic current mode), to be composed of two non-resolved peaks (labelled C and D) (Fig.…”

Section: Resultsmentioning

confidence: 99%

Capillary electrophoresis with mass spectrometric detection for separation of S-nitrosoglutathione and its decomposition products: a deeper insight into the decomposition pathways

et al. 2015

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S-Nitrosoglutathione (GSNO) is a very important biomolecule that has crucial functions in many physiological and physiopathological processes. GSNO acts as NO donor and is a candidate for future medicines. This work describes, for the first time, the separation and the detection of GSNO and its decomposition products using capillary electrophoresis coupled to mass spectrometry (CE-MS). The separation was performed in slightly alkaline medium (pH 8.5) under positive-ionization MS detection. The identification of three byproducts of GSNO was formally performed for the first time: oxidized glutathione (GSSG), glutathione sulfinic acid (GSO2H), and glutathione sulfonic acid (GSO3H). GSO2H and GSO3H are known to have important biological activity, including inhibition of the glutathione transferase family of enzymes which are responsible for the elimination of many mutagenic, carcinogenic, and pharmacologically active molecules. We observed, after the ageing of GSNO in the solid state, that the proportion of both GSSG and GSO3H increases whereas that of GSO2H decreases. These results enabled us to propose an oxidation scheme explaining the formation of such products.

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Section: Resultsmentioning

confidence: 99%

Capillary electrophoresis with mass spectrometric detection for separation of S-nitrosoglutathione and its decomposition products: a deeper insight into the decomposition pathways

et al. 2015

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“…Menstrual and cervical secretions and semen act as alkalizing agents to increase the vaginal pH [26,27] Speed of rotation 0, 2.5, and 5 rpm The dynamic movement was added on the system by shaking and rotating from vertical to horizontal position at various speeds [28,29] Site of application 7.5, 10, and 15 cm The loaded position of IVS determines the time required to achieve the effective NO concentration at the application site [30] IVS intravaginal system (i.e., drug delivery system), SVS simulating vaginal system (i.e., simulated organ for in vitro study), VFS vaginal fluid stimulant (i.e., synthetic vaginal fluids) environment for a prolonged delivery of NO. Poly(D,Llactic acid-co-glycolic acid) (PLGA) microparticles (MP) or nanoparticles were selected as carrier vehicles for DETA NONOate, considering that PLGA or polymer combinations is biocompatible and widely used as a biodegradable polymer [11,38]. Polymers would prevent DETA NONOate from decomposition at acidic pH, releasing NO at the mucosal sites in a controlled manner.…”

Section: Development Of a Microparticle For Nomentioning

confidence: 99%

“…MPs were suspended in two solutions; citrate buffer solution (pH 4.0) or PBS (pH 7.4) and kept in 37°C [11,38]. At a predetermined time interval, the solution was centrifuged and then the supernatant was removed.…”

Section: No Release Rates From Plga-based Mpsmentioning

confidence: 99%

“…The major challenge in intravaginal delivery of NO is a lack of delivery vehicles that allow for a localized and controlled delivery of NO under pH-sensitive conditions, in which they are initially exposed to acidic pH (i.e., normal healthy vagina pH 4.5) and later to male partner's semen (neutral pH) [8]. Various formulations including creams, patches, nanoparticles, and slow-release tablets are potential carriers for NO delivery through vaginal mucosa [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

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Treatment of blood flow abnormality using mucosal delivery of nitric oxide

Lee

2011

Drug Deliv. and Transl. Res.

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This review focuses on clinical application of intravaginal formulations containing nitric oxide (NO). Poly(D,L-lactic acid-co-glycolic acid)-based microparticles or nanoparticles encapsulated with nitric oxide prodrugs, such as diethylenetriamine diazeniumdiolate and S-nitrosoglutathione, have been developed for the treatment of blood flow abnormality in various diseases including diabetes. Advanced nanotechnology allows for production of novel formulations with the capability of long-term protection, preserving the integrity of the NO donors, and delivering NO in a controlled and sustained release manner at the mucosal sites. The gene expressions of MAPK and PKC in the vaginal mucosa upon exposure to microparticles were evaluated for the mechanistic study involved with blood flow changes. The blood flow changes and protein expression of the vaginal mucosa upon exposure to intravaginal formulations containing NO donors supported that NO therapy would be suitable for the treatment of blood flow abnormality. This review subsequently would help to establish a scientific foundation for clinical trials of intravaginal NO delivery systems in humans.

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“…The antifungal drugs clotrimazole (Sudeendra et al 2010) and itraconazole (ITR) (Dobaria et al 2009) were both formulated as vaginal films for treatment of vaginal candidiasis. A film formulation was studied which contains s-nitrosoglutathione, an endogenous NO donor, for use in female sexual arousal disorder (FSAD) (Yoo et al 2009).…”

Section: The History and Background Of Polymeric Thin Filmsmentioning

confidence: 99%

Microbicide Dosage Forms

Rohan

Devlin

Yang

2013

Current Topics in Microbiology and Immunology

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Microbicides are topically applied, user controlled dosage forms that are being developed to prevent the transmission of HIV during coitus. Early candidates focused on coitally dependent dosage forms such as gels and creams. More recent development has focused on broadening the coitally dependent options through the introduction of films and fast dissolving tablets. Additionally, it has become important to have longer acting products to minimize the burden of user compliance and thus vaginal rings have been developed providing sustained delivery of antiretroviral drugs. This chapter discusses the history of microbicides along with a detailed description of coitally dependent products, gels, films, tablets diaphragms, as well as coitally independent dosage forms such as vaginal rings and the introduction of a new technology, electrospun fibers.

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In vivo evaluation of vaginal films for mucosal delivery of nitric oxide

Cited by 50 publications

References 39 publications

Capillary electrophoresis with mass spectrometric detection for separation of S-nitrosoglutathione and its decomposition products: a deeper insight into the decomposition pathways

Capillary electrophoresis with mass spectrometric detection for separation of S-nitrosoglutathione and its decomposition products: a deeper insight into the decomposition pathways

Treatment of blood flow abnormality using mucosal delivery of nitric oxide

Microbicide Dosage Forms

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