In Vivo Evaluation of the Genotoxic Effects of Gonadotropins on Rat Reticulocytes

Abstract

In rats, the micronucleus genotoxicity assay suggests a dose-dependent gonadotropin effect on genomic instability in bone marrow stem cells in vivo.

The determination of possible genetic damage to women undergoing in vitro fertilization due to infertility caused by the male factor

“…For this purpose, they administrated CC (in concentrations of 0.16, 0.32, and 0.64 mg/kg/ day bw) to rats intraperitoneally for 5 days, and they reported a dosedependent increase in micronucleus frequency in bone-marrow stem cells. Duran et al (2011) evaluated the genotoxic effects of gonadotropins on rat reticulocytes in an in situ MN assay. They observed statistically significant differences among all parameters of cell abnormalities and MN cells except for the anaphase bridge abnormality by using high doses of subcutaneous gonadotropins.…”

“…In another study, Elbling and Colot (1987) showed that superovulation with gonadotropins increased the rates of SCE in newborn mice. Duran et al (2011) investigated the in vivo genotoxic effects of gonadotropins on rat reticulocytes with the micronucleus test, and they reported that gonadotropins increased micronucleus frequencies dose dependently. In addition, Dursun et al (2006) and Xu et al (2011) reported that high concentrations of FSH in in vitro maturation (IVM) medium significantly increased the first meiotic division error, resulting in more aneuploid oocytes during IVM.…”