In Vivo Evaluation of the Wound Healing Activity of Extracts and Bioactive Constituents of the Marine Isopod Ceratothoa oestroides

Abstract

Wound healing is a fundamental response to tissue injury and a number of natural products has been found to accelerate the healing process. Herein, we report the preparation of a series of different polarity (organic and aqueous) extracts of the marine isopod Ceratothoa oestroides and the in vivo evaluation of their wound healing activity after topical administration of ointments incorporating the various extracts on wounds inflicted on SKH-hr1 hairless mice. The most active extract was fractionated for enrichment in the bioactive constituents and the fractions were further evaluated for their wound healing activity, while their chemical profiles were analyzed. Wound healing was evaluated by clinical assessment, photo-documentation, histopathological analysis and measurement of biophysical skin parameters, such as transepidermal water loss (TEWL), hydration, elasticity, and skin thickness. The highest levels of activity were exerted by treatment of the wounds with a fraction rich in eicosapentaenoic acid (EPA), as well as myristic and palmitoleic acids. Topical application of the bioactive fraction on the wounds of mice resulted in complete wound closure with a skin of almost normal architecture without any inflammatory elements.

Ointments containing Ceratothoa oestroides extract: Evaluation of their healing potential in the treatment of diabetic foot ulcers

“…The isopod Ceratothoa oestroides is a protandric hermaphrodite parasite mainly found in the oral cavity of sea bass Dicentrarchus labrax and sea bream Sparus aurata . The olive oil extract of C. oestroides has previously demonstrated remarkable wound healing efficacy without apparent toxic effects on animal models . Similarly, a significant healing activity of the extract in second degree burns has been reported .…”

Ucuùba Fat Characterization and Use to Obtain Lipid Nanoparticles by High‐Pressure Homogenization with Full Factorial Design

“…Myristic acid is a 14‐carbon chain saturated fatty acid widely distributed in fats throughout the animal and plant kingdoms 22. Topically applied on the skin, myristic acid proved to have bactericidal activity against S. epidermidis 23 and wound healing activity 24.…”

“…Myristic acid is a 14-carbon chain saturated fatty acid widely distributed in fats throughout the animal and plant kingdoms [22]. Topically applied on the skin, myristic acid proved to have bactericidal Oxidative stability index (OSI), 110 °C > 20 h activity against S. epidermidis [23] and wound healing activity [24]. Lauric acid is a saturated medium-chain fatty acid (C:12) also present in Babassu, coconut, and Tucuma ˜oil [2,18].…”

Exploration of the role of a lithophytic fern, Pteris vittata L. in wound tissue regeneration and remodelling of genes in hyperglycaemic rat model

“…GC-MS analysis of fraction 1 of PV led to the elucidation of eight to ten compounds, which were both aliphatic and aromatic in nature. The molecular formula and weights of the compounds isolated from PV were mainly 3-Hexadecene (C 16 [49], Stearic and Oleic acids are known to be associated with modulation of immune response in wound healing [50] and these fatty acids accelerate the inflammatory phase of wound healing [51]. L-Ascorbyl 2, 6-Dipalmitate (Ascorbic acid) one of the active compounds present is reported to be responsible for the antioxidant activity.…”