In Vivo Evaluation of Porous Hydroxyapatite/Poly D/L-lactide Composite for Bone Substitutes and Scaffolds

Abstract

We had investigated the biocompatibility, osteoconductivity, and biodegradability of a porous composite of hydroxyapatite (HA) and poly-DL-lactide (PDLLA) implanted into rabbit femoral condyles. It showed excellent osteoconductivity and biodegradability as a bone substitute. Newly formed bones were remodeled, and materials were resorbed almost completely at 78weeks after implantation. In consideration of its biocompatibility and degradability, we investigated its potential for use as a cellular scaffold and evaluated its osteoinductive property. On implantation to the rat dorsal subcutaneous tissue loaded with syngeneic bone marrow cells, osteogenesis with enchondral ossification was seen both on and in the material at 3 weeks after implantation. This osteogenesis in the HA/PDLLA tended to get mature and newly formed bone tissues were found in the material by 6weeks. To investigate the osteoinductive property material itself has, we attempted to implant this porous composite material to extra-osseous canine dorsal muscle. At 2months, osteogenesis was seen in the pores of the material. It indicated the material induced osteogenesis with intramembranous ossification process. Therefore, HA/PDLLA might be a desirable material for bone substitutes and cellar scaffolds with osteoconductive and osteoinductive property.

Feasibility of a Three-Dimensional Porous Uncalcined and Unsintered Hydroxyapatite/poly-d/l-lactide Composite as a Regenerative Biomaterial in Maxillofacial Surgery

“…This is useful in the context of irregular bone reformation in maxillofacial surgery. According to Hasegawa et al [ 6 , 11 ], porous 3D-HA/PDLLA biomaterial resorbs much more rapidly than HA or β-TCP. This is very important for bone formation, where Chazono et al [ 46 ] previously reported that bone formation occurred after biomaterial resorption.…”

“…The pores of the 3D-HA/PDLLA composite are interconnected with an average interconnective pore diameter of 62 μm. The 3D-HA/PDLLA biomaterial was composed of 70 wt% uncalcined and unsintered HA (u-HA) and 30 wt% PDLLA matrix, with 4.1 ± 0.4 MPa compressive strength (viscosity-average molecular weight (Mv): 77 kDa; dextrorotatory-lactide acid/levorotatory-lactide acid ( d / l ) = 50/50 mol%) and without immediate collapse after breaking [ 11 ].…”

“…Previous studies concluded that the mechanical strength and strength retention of 3D-HA/PDLLA recommend its use for the reconstruction of long bones, such as the femoral condyles [ 11 ]. However, such results have not been demonstrated in irregular bones, such as the maxillofacial region.…”

Regularities of obtaining and propertіеs of the hydroxyapatite filled porous composites based on polyvinylpyrrolidone

“…Ключевые слова: пористые гидрогели, поливинилпирролидон, наночастицы серебра, гидроксиаппатит, бактерицидные и фунгицидные свойства DOI: 10.15587/1729-4061.2014.27701 кісткової тканини внаслідок різних хірургічних втручань, проведення імплантологічного лікування, а також різних кістково пластичних операцій [3]. Для вирішення цих проблем переважно застосовують ся різні вітчизняні і зарубіжні препарати, створені на основі гідроксіапатиту та трикальційфосфату, що містять хімічні елементи в таких же йонних формах, в яких вони знаходяться в живому організмі [4,5].…”

Application of a Bioactive/Bioresorbable Three-Dimensional Porous Uncalcined and Unsintered Hydroxyapatite/Poly-d/l-lactide Composite with Human Mesenchymal Stem Cells for Bone Regeneration in Maxillofacial Surgery: A Pilot Animal Study

“…3D-HA/PDLLA has been successfully implanted into animal femora and tibia [ 8 , 9 , 10 ]. Moreover, ectopic osteogenesis was observed in the implantation of 3D-HA/PDLLA loaded with bone marrow cells into muscles [ 11 ]. Therefore, it could potentially be developed for clinical applications as a novel bioresorbable scaffold in maxillofacial surgery.…”