In Vivo Evaluation of Permeable and Impermeable Membranes for Guided Bone Regeneration

Abstract

Background: The degree of biodegradation and the inflammatory response of membranes employed for guided bone regeneration directly impact the outcome of this technique. This study aimed to evaluate four different experimental versions of Poly (L-lactate-co-Trimethylene Carbonate) (PTMC) + Poly (L-lactate-co-glycolate) (PLGA) membranes, implanted in mouse subcutaneous tissue, compared to a commercially available membrane and a Sham group. Methods: Sixty Balb-C mice were randomly divided into six experimental groups and subdivided into 1, 3, 6 and 12 weeks (n = 5 groups/period). The membranes (1 cm2) were implanted in the subcutaneous back tissue of the animals. The samples were obtained for descriptive and semiquantitative histological evaluation (ISO 10993-6). Results: G1 and G4 allowed tissue adhesion and the permeation of inflammatory cells over time and showed greater phagocytic activity and permeability. G2 and G3 detached from the tissue in one and three weeks; however, in the more extended periods, they presented a rectilinear and homogeneous aspect and were not absorbed. G2 had a major inflammatory reaction. G5 was almost completely absorbed after 12 weeks. Conclusions: The membranes are considered biocompatible. G5 showed a higher degree of biosorption, followed by G1 and G4. G2 and G3 are considered non-absorbable in the studied periods.

The influence of electrical high-speed rotation on mandibular third molar surgeries: a prospective, randomized, split-mouth clinical and radiographic study

“…For this purpose, the bone biopsies performed underwent standard histological processing (fixation in 10% formaldehyde, demineralization in EDTA, dehydration in alcohol sequence, clearing with xylene and inclusion in paraffin). Microtomy with serial sections of 4 μm thickness were obtained with subsequent staining in hematoxylin and eosin (HE) for histological and histomorphometric analysis of the necrosis range and regularity of the bone edge (smooth, slightly irregular and irregular) 4 , 19 . After obtaining the slides, images were captured at 25 × optical microscopic magnification using a digital camera attached to the optical microscope (PrimeCam, NPlus 12, Nikon, Florida, USA) and connected to a microcomputer, for analysis using the ImageJ software (National Institutes of Health, Bethesda, USA).…”