New strategies for fast, efficient and environmentally friendly approaches in nanoparticle (NP) synthesis have increased the interest in less toxic green synthesis products. In this study, VA-Pd / AC nanoparticles (NPs) were synthesized using palladium (Pd), activated carbon (AC) and Viscum album (VA) aqueous extract as stabilizer / reductant. Activated carbon in the synthesis stage was obtained using the Chenopodium album (CA) plant. The structural and morphological properties of VA-Pd / AC NPs were examined using TEM and XRD analyses. Zebrafish embryo and larvae were exposed to the produced VA-Pd / AC NPs at different concentrations (10, 50, 100 and 500 mg/L) for 96 h. Embryos/larvae were examined for survival rate, hatching and malformation during development. Morever, potential neurotoxic effects were assessed histopathologically and immunohistochemically using Caspase 3 and nNOS indicators. Although the hatching rate of VA-Pd / AC NP was 100% at all concentrations, the survival rate decreased in a dose dependent manner. Histopathological analyses revealed that 10 mg/L group looked similar to the control group, whereas necrotic degenerations were observed in the 50, 100 and 500 mg/L groups in a dose- dependent manner. In addition, Caspase 3 and nNOS expressions were evaluated as negative in the control and 10 mg/L groups in immunofluorescence staining. These results provide the evidence that 500 mg/L concentration of green synthesized VA-Pd/AC NPs from Viscum album (VA) and Chenopodium album (CA) plant induces neurotoxic and tetragenonic effect on zebrafish.