In Vivo Evaluation of Magnetic Targeting in Mice Colon Tumors with Ultra-Magnetic Liposomes Monitored by MRI

Thébault, Caroline; Ramniceanu, Gregory; Michel, Aude; Beauvineau, Claire; Girard, Christian; Séguin, Johanne; Mignet, Nathalie; Ménager, Christine; Doan, Bich‐Thuy

doi:10.1007/s11307-018-1238-3

Cited by 16 publications

(18 citation statements)

References 39 publications

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“…Citrate-coated γ-Fe 2 O 3 (cit-Fe 2 O 3 ) MNPs used in this work was synthesized by the co-precipitation method as described in our previous studies [ 22 ] ( Figure 2 , Table S1 ). All the lipids were dissolved in CHCl 3 (Carlo Erba Reagents, Val-de-Reuil, France) at a concentration of 25 mg/mL.…”

Section: Methodsmentioning

confidence: 99%

“…Previously, we successfully prepared ultra-magnetic liposomes (UMLs) by a reverse phase evaporation method, which gave a higher MNPs loading efficiency (about 100 folds) compared to the classical thin film hydration method [ 20 , 21 ]. Thanks to high content of MNPs, these vehicles are able to give high T2 MRI contrast enhancement and targeting effect in vivo [ 22 ]. Based on these positive results, in this work, we ought to obtain magnetic lipoplexes that would benefit from targeting efficacy and MRI contrast enhancement.…”

Section: Introductionmentioning

confidence: 99%

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Development of Theranostic Cationic Liposomes Designed for Image-Guided Delivery of Nucleic Acid

et al. 2020

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Cationic liposomes have been considered as potential vectors for gene delivery thanks to their ability to transfect cells with high efficiency. Recently, the combination of diagnostic agent and therapeutic agents in the same particle to form a theranostic system has been reported. Magnetic liposomes are one of these examples. Due to the magnetic nanoparticles encapsulated in the liposomes, they can act as a drug delivery system and, at the same time, a magnetic resonance imaging contrast enhancement agent or hyperthermia. In this work, nucleic acid delivery systems based on magnetic cationic liposomes (MCLs) were developed. Two different techniques, reverse phase evaporation and cosolvent sonication, were employed for liposome preparation. Both strategies produced MCLs of less than 200 nm with highly positive charge. Enhancement of their transverse and longitudinal relaxivities r2 and r1 was obtained with both kinds of magnetic liposomes compared to free magnetic nanoparticles. Moreover, these MCLs showed high capacity to form complexes and transfect CT-26 cells using the antibiotic-free pFAR4-luc plasmid. The transfection enhancement with magnetofection was also carried out in CT26 cells. These results suggested that our MCLs could be a promising candidate for image-guided gene therapy.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of Theranostic Cationic Liposomes Designed for Image-Guided Delivery of Nucleic Acid

et al. 2020

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“…Among them, magnetic liposomes, also called Ultra Magnetic Liposomes (UML) because they contain a high volume ratio of magnetic nanoparticles, have been developed [11] . Due to their magnetic charge which was previously determined by magnetization measurements around 20% in volume fraction, UML have shown the ability to be accumulated in xenografted murine colon tumors with a factor 3 increase as regard to non-targeted liposomes [12]. Despite the presence of magnetic nanoparticles there is enough free space to expect a high loading of CA4P in the aqueous part of the liposomes.…”

Section: Introductionmentioning

confidence: 91%

“…The normalized volume of the tumor was then calculated at each time point by dividing the volume of the tumor by one of the tumor at D0. To compare the efficiency of accumulation of the CA4P-UML depending on the treatment group, the previously described post-processing methodology %I0.25 was used [12]. Briefly, the pixel intensity distribution of each tumor was obtained from the T2-weighted images and the percentage of pixels under the value I0.25= 0.25x(Intensitymax-Intensitymin) was calculated and noted %I0.25.…”

Section: Mri Data Analysismentioning

confidence: 99%

“…Despite the presence of magnetic nanoparticles there is enough free space to expect a high loading of CA4P in the aqueous part of the liposomes. Considering the magnetic relaxivities for MRI, encapsulation of magnetic nanoparticles in ULM provides cooperating MNP and gives a higher magnetic moment, resulting in a higher proton spin dephasing with shortened T2 value [12] allows to multiply by a factor of 3 the r2 relaxivity and by a factor of 5 the r2:r1 ratio, leading to a far more efficient contrast agent for MRI. Another interest of magnetic nanocarriers, is the possibility to trigger the release in a controlled manner.…”

Section: Introductionmentioning

confidence: 99%

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Theranostic MRI liposomes for magnetic targeting and ultrasound triggered release of the antivascular CA4P

Thébault

Ramniceanu

Boumati

et al. 2020

Journal of Controlled Release

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HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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Near‐Infrared Emission‐Cyanine 5.5 and Doxorubicin Loaded Magnetite Nanoparticles for Dual In Vivo Magnetic Resonance and Biodistribution Imaging

Son Phan,

Thuy Doan,

Thu Huong Le

et al. 2023

ChemistrySelect

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The in vivo imaging of the drug distribution during cancer treatment plays an important role in helping physicians and pharmacists to monitor the progress of treatment and improve the effectiveness of therapy. Our goal in this work was to prepare a multifunctional nano drug delivery system that not only co‐loaded iron oxide nanoparticles (Fe3O4 NPs) and Doxorubicin (Dox) but also attached the near‐infrared fluorescent agent Cyanine 5.5 based on poly(lactide)‐tocopheryl polyethylene glycol 1000 succinate (PLA‐TPGS) copolymer. Cyanine 5.5 was attached to the surface Fe3O4 NPs via the NH−CO chemical bond, followed by co‐loading of Dox onto the PLA‐TPGS copolymer by the emulsion solvent evaporation method. The structural, morphological, dimensional, stability, and optical‐magnetic properties of the as‐synthesized nanosystem were fully characterized. From the drug‐released study, the prolonged release of Dox accelerated with increasing acidic conditions (at pH 5.0 and 6.5), while a slow release was observed at pH 7.4. Through the MTT assay, the nanosystem had cellular toxicity against Hep−G2 and HeLa cell lines with low IC50 values of 0.42 and 0.78 μg.mL−1 in terms of Dox concentration, respectively. At a magnetic field of 7 T, this nanosystem also exhibited a high r2/r1 ratio of 164.5 and a strong contrast magnetic resonance (MR) image in the liver. In vivo biodistribution studies revealed that the highest fluorescence intensity achived in the liver after 6 h, and the nanosystem was eliminated from the body after at least 24 h. Collectively, these results demonstrated that the PLA‐TPGS‐Fe3O4‐Cyanine 5.5‐Dox nanosystem is a good candidate for simultaneously increasing contrast for MR and near‐infrared fluorescence imaging.

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In Vivo Evaluation of Magnetic Targeting in Mice Colon Tumors with Ultra-Magnetic Liposomes Monitored by MRI

Abstract: We have developed a method that allows a semi-quantitative evaluation of targeting efficiency in tumors, which could be applied to different T contrast agents.

Cited by 16 publications

References 39 publications

Development of Theranostic Cationic Liposomes Designed for Image-Guided Delivery of Nucleic Acid

Development of Theranostic Cationic Liposomes Designed for Image-Guided Delivery of Nucleic Acid

Theranostic MRI liposomes for magnetic targeting and ultrasound triggered release of the antivascular CA4P

Near‐Infrared Emission‐Cyanine 5.5 and Doxorubicin Loaded Magnetite Nanoparticles for Dual In Vivo Magnetic Resonance and Biodistribution Imaging

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