In vivo evaluation of antitumoral and antiangiogenic effect of imiquimod-loaded polymeric nanoparticles

Dias, Marina França; Figueiredo, Bruna Caroline Pinheiro de; Teixeira-Neto, Júlia; Guerra, Maria Carolina Andrade; Fialho, Sílvia Ligório; Silva-Cunha, Armando

doi:10.1016/j.biopha.2018.04.079

Cited by 29 publications

(23 citation statements)

References 35 publications

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“…Since we have detected a different response to a vasoconstriction stimulus, we can speculate that the application of topical IMI may act also at a vascular level. Accordingly, a few recent studies have highlighted an antiangiogenic effect of topical IMI, thus suggesting a possible use in the treatment of hemangiomas [ 32 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

Non-Invasive Analysis of Actinic Keratosis before and after Topical Treatment Using a Cold Stimulation and Near-Infrared Spectroscopy

et al. 2020

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Background and objectives: The possible evolution of actinic keratoses (AKs) into invasive squamous cell carcinomas (SCC) makes their treatment and monitoring essential. AKs are typically monitored before and after treatment only through a visual analysis, lacking a quantitative measure to determine treatment effectiveness. Near-infrared spectroscopy (NIRS) is a non-invasive measure of the relative change of oxy-hemoglobin and deoxy-hemoglobin (O2Hb and HHb) in tissues. The aim of our study is to determine if a time and frequency analysis of the NIRS signals acquired from the skin lesion before and after a topical treatment can highlight quantitative differences between the AK skin lesion area. Materials and Methods: The NIRS signals were acquired from the skin lesions of twenty-two patients, with the same acquisition protocol: baseline signals, application of an ice pack near the lesion, removal of ice pack and acquisition of vascular recovery. We calculated 18 features from the NIRS signals, and we applied multivariate analysis of variance (MANOVA) to compare differences between the NIRS signals acquired before and after the therapy. Results: The MANOVA showed that the features computed on the NIRS signals before and after treatment could be considered as two statistically separate groups, after the ice pack removal. Conclusions: Overall, the NIRS technique with the cold stimulation may be useful to support non-invasive and quantitative lesion analysis and regression after a treatment. The results provide a baseline from which to further study skin lesions and the effects of various treatments.

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Section: Discussionmentioning

confidence: 99%

Non-Invasive Analysis of Actinic Keratosis before and after Topical Treatment Using a Cold Stimulation and Near-Infrared Spectroscopy

et al. 2020

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“…Each animal's dorsal skin was clipped 48 h before the experiment began in order to remove a 3 × 3 cm 2 area. A single dosage of the tumor initiator DMBA (1 mg in 200 µL acetone) was given subcutaneously to each mouse to generate a tumor [33]. Increased epidermal tumors which were larger and progressed more frequently to malignant carcinoma known as papillomas were observed after the injection of DMBA.…”

Section: In Vivo Anti-tumor Characterization Of Sle Gel Formulation 261 Study Designmentioning

confidence: 99%

Improving the Antitumor Activity and Bioavailability of Sonidegib for the Treatment of Skin Cancer

et al. 2021

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Throughout the United States and the world, skin cancer is the most frequent form of cancer. Sonidegib (SNG) is a hedgehog inhibitor that has been used for skin cancer treatment. However, SNG has low bioavailability and is associated with resistance. The focus of this work is to enhance bioavailability, anti-tumor efficacy and targeting of SNG via developing ethosome gel as a potential treatment for skin cancer. SNG-loaded ethosomes formulation was prepared and characterized in vitro by %entrapment efficiency (%EE), vesicle size, morphology, %release and steady-state flux. The results showed that the prepared formulation was spherical nanovesicles with a %EE of 85.4 ± 0.57%, a particle size of 199.53 ± 4.51 nm and a steady-state flux of 5.58 ± 0.08 µg/cm2/h. In addition, SNG-loaded ethosomes formulation was incorporated into carbopol gel to study the anti-tumor efficacy, localization and bioavailability in vivo. Compared with oral SNG, the formulation showed 3.18 times higher relative bioavailability and consequently significant anti-tumor activity. In addition, this formulation showed a higher rate of SNG penetration in the skin’s deep layers and passive targeting in tumor cells. Briefly, SNG-loaded ethosome gel can produce desirable therapeutic benefits for treatment of skin cancer.

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“…The dorsal skin of each animal was clipped 48 hours before the experiment to remove a 3 × 3 cm 2 section. To create a tumour, each mouse was given a single dose of the tumour initiator DMBA (1 mg in 200 L acetone) [28]. Following the injection of DMBA, epidermal tumours became bigger and more commonly proceeded to malignant carcinoma (known as papilloma).…”

Section: In Vitro Characterization Of Optimum Rldl Formulation Gelmentioning

confidence: 99%

“…The quantification of the number and diameter of papilloma > 1 mm were investigated weekly to measure the antitumour activity of the RLDL formulation [28]. Additionally, histopathology estimation was obtained to confirm the antitumour activity and toxicity of the RLDL formulation.…”

Section: Antitumour Activity and Toxicity Determinationmentioning

confidence: 99%

“…The skin was the homogenised for 10 min at 8000 rpm and the tissue homogenate (1 mL) was mixed with acetonitrile before being centrifuged for 10 min at 3.0× g [32]. The supernatant was evaporated and dissolved in the mobile phase for HPLC analysis in triplicate to determine the drug concentration [28]. The student's t-test was used to assess the statistical analysis.…”

Section: In Vivo Permeation and Bioavailability Studiesmentioning

confidence: 99%

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The Impact of Improving Dermal Permeation on the Efficacy and Targeting of Liposome Nanoparticles as a Potential Treatment for Breast Cancer

et al. 2021

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Breast cancer is the most frequent malignancy in women. This work focuses on developing deformable liposomes as a potential carrier for breast cancer treatment and studying the impact of improving dermal permeation on the efficacy and targeting of liposomes. Raloxifene (RXF), an oestrogen antagonist, was used as a model drug. Using Box–Behnken design, different formulations of RXF-loaded deformable liposome (RLDL) were prepared using different propylene glycol, phospholipid and cholesterol concentrations. The percentage of entrapment efficiency (Y1), particle size (Y2), zeta potential (Y3) and steady-state flux (Y4) of the prepared formulations were all evaluated. Y1 and Y4 were significantly increased and Y2 and Y3 were significantly decreased when the propylene glycol concentration was increased. The optimization was obtained and the optimum formulation was that including phospholipid (1.40% w/w), cholesterol (0.15% w/w) and propylene glycol (10% v/v). The selected optimum formulation displayed a % EE of 78.34 ± 1.04% with a steady-state flux of 4.21 ± 0.02 µg/cm2/h. In order to investigate bioavailability, antitumor effectiveness and permeation, the optimum formulation was selected and included in a carbopol gel. The optimum gel formulation had 2.77 times higher bioavailability and, as a result, considerable antitumor action as compared to oral RXF. In conclusion, optimum RLDL gel may be an effective breast cancer treatment.

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In vivo evaluation of antitumoral and antiangiogenic effect of imiquimod-loaded polymeric nanoparticles

Cited by 29 publications

References 35 publications

Non-Invasive Analysis of Actinic Keratosis before and after Topical Treatment Using a Cold Stimulation and Near-Infrared Spectroscopy

Non-Invasive Analysis of Actinic Keratosis before and after Topical Treatment Using a Cold Stimulation and Near-Infrared Spectroscopy

Improving the Antitumor Activity and Bioavailability of Sonidegib for the Treatment of Skin Cancer

The Impact of Improving Dermal Permeation on the Efficacy and Targeting of Liposome Nanoparticles as a Potential Treatment for Breast Cancer

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