In-vivo Estimation of Optimized Floating Microspheres Design by QBD approach

Abstract

In order to regulate its release, avoid repeated dosing, and consequently maximize bioavailability, it is essential to develop and construct floating microspheres of glipizide. According to Strubel et al., HPMC and EC were combined to form glipizide-loaded floating microspheres using a solvent evaporation technique. Drug loading, particle size, and percent drug release were used as response factors in the formulation optimization process, which took into account polymer concentration and stirring rate as independent variables.The bulk density, tapped density, flowability, and particle size of the formulation batches were all evaluated. Its in-vitro performance was assessed using the standard pharmacopoeal assays as well as other procedures, including yield (%), drug polymer compatibility (FTIR scan), and tapped density (%). Analysis of compressibility particle size, drug entrapment effectiveness, surface topography (SEM), and an in vitro release research. with a yield of 88.30±0.53%, an ideal drug loading of 85.50±0.55%, and a preferred buoyancy of 91.93±0.61%, tiny to big and spherical microspheres were created at 40°C using a 1:6 polymer, 2% Tween 80, and that temperature. The development of a stable emulsion and equally sized internal phase droplets was largely dependent on the emulsifying agent. A crucial element in the emulsification process is the propeller's stirring speed. Additionally, it influences the droplets' size and shape, which has an impact on the microspheres. The decision was made to incorporate stirring rate and polymer concentration as independent variables in the experimental design while keeping the other variables fixed as a result of the debate that was just had. Measurements of the hypoglycemic response brought on by oral administration were used to determine the in vivo effectiveness of the optimized batch in healthy normal Wistar rats (250–300gm).