In Vivo Ergogenic Properties of the Bifidobacterium longum OLP-01 Isolated from a Weightlifting Gold Medalist

Abstract

In recent years, probiotics of human origin have shown superior results and performance compared to probiotics from plant or dairy sources, in both in vitro and animal studies. Towards this end, the current study was conducted to explore the ergogenic properties of Bifidobacterium longum subsp. longum OLP-01 isolated from the intestinal microbiome of the gold medalist from the 2008 Beijing Olympics women’s 48 kg weightlifting competition. Male Institute of Cancer Research (ICR) mice were divided into four groups (n = 10 per group) and orally administered OLP-01 for 4 weeks at 0 (vehicle), 2.05 × 109 (OLP-01-1X), 4.10 × 109 (OLP-01-2X), and 1.03 × 1010 (OLP-01-5X) CFU/kg/day. Physical performance tests including grip strength and endurance time were measured, with OLP-01 supplementation dose-dependently elevating grip strength and endurance. The anti-fatigue activity levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) were measured after an acute exercise challenge, and OLP-01 was found to significantly decrease lactate, ammonia, and CK levels. OLP-01 treatment was also found to significantly increase the resting levels of both hepatic and muscular glycogen, an indicator of energy storage. Supplementation by OLP-01 showed no subchronic toxic effects while supporting many health-promoting, performance-improving, and fatigue-ameliorating functions.

Bifidobacterium longum subsp. longum OLP-01 Supplementation during Endurance Running Training Improves Exercise Performance in Middle- and Long-Distance Runners: A Double-Blind Controlled Trial

“…The probiotic OLP-01 isolated from the 2008 Olympic women's 48 kg weightlifting gold medalist's gut microbiota has been proven in past animal experiments to effectively improve exercise endurance performance and reduce fatigue indicators [28,29]. In this study, after the well-trained At the Genus level ( Figure 3B), no significant differences between placebo and OLP-01 groups existed before the experimental intervention.…”

Lactobacillus salivarius Subspecies salicinius SA-03 is a New Probiotic Capable of Enhancing Exercise Performance and Decreasing Fatigue

“…Previous studies have shown that supplementation of Saccharomyces boulardii in rats undergoing accelerated exercise increased maximum oxygen consumption by 12.7%, maximum aerobic speed by 12.4%, and running time to fatigue by 21.6%, compared to with sedentary the control group [40]. In our previous study, the Bifidobacterium longum (OLP-01) isolated from a weightlifting gold medalist found that, not only help in fatigue indicators, but also significantly increased 1.77-3.37-fold exercise to exhaustive time by 1X, 2X and 5X dose OLP-01 supplementation for four weeks, in addition, it also has a significant increase in forelimb grip, although there is no significant difference in muscle mass [16]. Lactobacillus plantarum (TWK10) supplement not only improves exercise performance and increases muscle mass in mice [15], but also increased endurance performance and elevated blood glucose concentration following exercise-to-exhaustion after six weeks of high dose (1 × 10 11 CFU) TWK10 supplementation in untrained healthy male adults [41].…”

“…According to the method previously used in our laboratory, immediately after euthanizing the mice, the collected samples were stored at -80 • C for DNA extraction. Detailed procedures for sample extraction, preparation and analysis have been previously described [16].…”

“…They have also been shown to enhance carbohydrate metabolism to produce SCFA [13] such as butyrate, which is then converted to acetyl-CoA for production of adenosine triphosphate (ATP) to provide energy [14]. Therefore, past research has shown that, probiotic supplements can effectively improve exercise performance and reduce fatigue biochemical values after exercise [15,16].…”

Gut Microbiota, Probiotics and Physical Performance in Athletes and Physically Active Individuals

“…Recently, the oral administration of either B. longum subsp. longum OLP-01 [ 98 ] or Ligilactobacillus salivarius subsp. salicinius SA-03 [ 99 ], isolated from a female weightlifting Olympic medalist, was demonstrated to significantly increase forelimb grip strength and endurance capacity in a swim-to-exhaustion test in mice.…”