In vivo endocrine disruption assessment of wastewater treatment plant effluents with small organisms

Castillo, Luis; Seriki, Kemi; Matéos, Stéphanie; Loire, Nicolas; Guédon, Nathalie; Lemkine, Gregory F.; Demeneix, Barbara; Tindall, Andrew J.

doi:10.2166/wst.2013.179

Cited by 15 publications

(6 citation statements)

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“…Using oligo microarray and qPCR analyses, they found that WWE and T 3 exposures significantly increased the expression of TH-sensitive genes: thrb, dio2, crhbp , and fap (Table 4). The in vivo effects of WWE on TH-linked gene expression was also demonstrated by Castillo et al in a transgenic X. laevis harboring a thibz -GFP reporter construct that was activated by WWE exposure (Table 4) (188).…”

Section: Complex Mixturessupporting

confidence: 54%

Contaminant and Environmental Influences on Thyroid Hormone Action in Amphibian Metamorphosis

et al. 2019

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Aquatic and terrestrial environments are increasingly contaminated by anthropogenic sources that include pharmaceuticals, personal care products, and industrial and agricultural chemicals (i. e., pesticides). Many of these substances have the potential to disrupt endocrine function, yet their effect on thyroid hormone (TH) action has garnered relatively little attention. Anuran postembryonic metamorphosis is strictly dependent on TH and perturbation of this process can serve as a sensitive barometer for the detection and mechanistic elucidation of TH disrupting activities of chemical contaminants and their complex mixtures. The ecological threats posed by these contaminants are further exacerbated by changing environmental conditions such as temperature, photoperiod, pond drying, food restriction, and ultraviolet radiation. We review the current knowledge of several chemical and environmental factors that disrupt TH-dependent metamorphosis in amphibian tadpoles as assessed by morphological, thyroid histology, behavioral, and molecular endpoints. Although the molecular mechanisms for TH disruption have yet to be determined for many chemical and environmental factors, several affect TH synthesis, transport or metabolism with subsequent downstream effects. As molecular dysfunction typically precedes phenotypic or histological pathologies, sensitive assays that detect changes in transcript, protein, or metabolite abundance are indispensable for the timely detection of TH disruption. The emergence and application of ‘omics techniques—genomics, transcriptomics, proteomics, metabolomics, and epigenomics—on metamorphosing tadpoles are powerful emerging assets for the rapid, proxy assessment of toxicant or environmental damage for all vertebrates including humans. Moreover, these highly informative ‘omics techniques will complement morphological, behavioral, and histological assessments, thereby providing a comprehensive understanding of how TH-dependent signal disruption is propagated by environmental contaminants and factors.

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Section: Complex Mixturessupporting

confidence: 54%

Contaminant and Environmental Influences on Thyroid Hormone Action in Amphibian Metamorphosis

et al. 2019

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“…Thyroid hormone signaling is essential for metabolism, growth, and organ development, including the brain. , While some studies reported thyroid receptor α (TRα)activation by (un)treated wastewater, , only four studies (5 assays) included advanced wastewater treatment technologies and detected no thyroid activity in the effluents of the CT or the advanced treatment. ,,, In contrast, in vivo studies with the Xenopus embryonic thyroid assay (XETA) suggest the occurrence of thyroid disruptors in conventionally treated wastewater, − which is not surprising, given that these compounds often act via non-receptor-mediated mechanisms . Thus, the XETA assay or in vitro bioassays for thyroid hormone biosynthesis (e.g., inhibition of the sodium-iodide symporter) are more relevant than TRα activation to investigate the removal of thyroid disruptors by (advanced) wastewater treatment.…”

Section: Removal Of In Vitro Toxicitymentioning

confidence: 99%

Systematic Review of Toxicity Removal by Advanced Wastewater Treatment Technologies via Ozonation and Activated Carbon

Völker

Stapf

Miehe

et al. 2019

Environ. Sci. Technol.

127

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Upgrading wastewater treatment plants (WWTPs) with advanced technologies is one key strategy to reduce micropollutant emissions. Given the complex chemical composition of wastewater, toxicity removal is an integral parameter to assess the performance of WWTPs. Thus, the goal of this systematic review is to evaluate how effectively ozonation and activated carbon remove in vitro and in vivo toxicity. Out of 2464 publications, we extracted 46 relevant studies conducted at 22 pilot or full-scale WWTPs. We performed a quantitative and qualitative evaluation of in vitro (100 assays) and in vivo data (20 species), respectively. Data is more abundant on ozonation (573 data points) than on an activated carbon treatment (162 data points), and certain in vitro end points (especially estrogenicity) and in vivo models (e.g., daphnids) dominate. The literature shows that while a conventional treatment effectively reduces toxicity, residual effects in the effluents may represent a risk to the receiving ecosystem on the basis of effect-based trigger values. In general, an upgrade to ozonation or activated carbon treatment will significantly increase toxicity removal with similar performance. Nevertheless, ozonation generates toxic transformation products that can be removed by a post-treatment. By assessing the growing body of effect-based studies, we identify sensitive and underrepresented end points and species and provide guidance for future research.

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“…At the organismal level, the thyroid system represents an important target of endocrine disruption (Boas, Feldt-Rasmussen, & Main, 2012;Brucker-Davis, 1998), thereby prompting development of the Xenopus metamorphosis assay for screening TH-disrupting chemicals (Opitz et al, 2005). A high-throughput, whole-tadpole method using transgenic animals that express green fluorescent protein upon exposure to TH-disrupting chemicals was also developed (Castillo et al, 2013;Fini et al, 2007). At the receptor level, the two TR isoforms, a and b, have distinct tissue-specific expression patterns (Cheng et al, 2010), such that use of TR antagonists and TR isoform-selective agonists may enable targeting specific tissues and avoid affecting off-target tissue-specific side-effects.…”

Section: Tool For Screening Th-active Compoundsmentioning

confidence: 99%

Frogs model man: In vivo thyroid hormone signaling during development

Sachs

Buchholz

2017

Genesis

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Thyroid hormone (TH) signaling comprises TH transport across cell membranes, metabolism by deiodinases, and molecular mechanisms of gene regulation. Proper TH signaling is essential for normal perinatal development, most notably for neurogenesis and fetal growth. Knowledge of perinatal TH endocrinology needs improvement to provide better treatments for premature infants and endocrine diseases during gestation and to counteract effects of endocrine disrupting chemicals. Studies in amphibians have provided major insights to understand in vivo mechanisms of TH signaling. The frog model boasts dramatic TH-dependent changes directly observable in free-living tadpoles with precise and easy experimental control of the TH response at developmental stages comparable to fetal stages in mammals. The hormones, their receptors, molecular mechanisms, and developmental roles of TH signaling are conserved to a high degree in humans and amphibians, such that with respect to developmental TH signaling "frogs are just little people that hop." The frog model is exceptionally illustrative of fundamental molecular mechanisms of in vivo TH action involving TH receptors, transcriptional cofactors, and chromatin remodeling. This review highlights the current need, recent successes, and future prospects using amphibians as a model to elucidate molecular mechanisms and functional roles of TH signaling during post-embryonic development.

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In vivo endocrine disruption assessment of wastewater treatment plant effluents with small organisms

Cited by 15 publications

References 0 publications

Contaminant and Environmental Influences on Thyroid Hormone Action in Amphibian Metamorphosis

Contaminant and Environmental Influences on Thyroid Hormone Action in Amphibian Metamorphosis

Systematic Review of Toxicity Removal by Advanced Wastewater Treatment Technologies via Ozonation and Activated Carbon

Frogs model man: In vivo thyroid hormone signaling during development

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