In vivo electroporation enhances the potency of poly-lactide co-glycolide (PLG) plasmid DNA immunization

Barbon, Christine M.; Baker, Lisa; Lajoie, Christa; Ramstedt, Urban; Hedley, Mary Lynne; Luby, Thomas M.

doi:10.1016/j.vaccine.2010.09.078

Cited by 15 publications

(15 citation statements)

References 43 publications

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“…Indeed, uric acid and high mobility group protein B1 (HMGB1) have been detected in electroporated muscle with or without vaccination and have been associated with robust T cell responses to plasmid DNA antigens 46 . Tissue damage and inflammation depend on EP parameters such as the intensity, the duration of electrical pulses and the type of electrodes 47, 48 .…”

Section: Discussionmentioning

confidence: 99%

Electroporation as a vaccine delivery system and a natural adjuvant to intradermal administration of plasmid DNA in macaques

Todorova

Adam

Čulina

et al. 2017

Sci Rep

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In vivo electroporation (EP) is used to enhance the uptake of nucleic acids and its association with DNA vaccination greatly stimulates immune responses to vaccine antigens delivered through the skin. However, the effect of EP on cutaneous cell behavior, the dynamics of immune cell recruitment and local inflammatory factors, have not been fully described. Here, we show that intradermal DNA vaccination combined with EP extends antigen expression to the epidermis and the subcutaneous skin muscle in non-human primates. In vivo fibered confocal microscopy and dynamic ex vivo imaging revealed that EP promotes the mobility of Langerhans cells (LC) and their interactions with transfected cells prior to their migration from the epidermis. At the peak of vaccine expression, we detected antigen in damaged keratinocyte areas in the epidermis and we characterized recruited immune cells in the skin, the hypodermis and the subcutaneous muscle. EP alone was sufficient to induce the production of pro-inflammatory cytokines in the skin and significantly increased local concentrations of Transforming Growth Factor (TGF)-alpha and IL-12. Our results show the kinetics of inflammatory processes in response to EP of the skin, and reveal its potential as a vaccine adjuvant.

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Section: Discussionmentioning

confidence: 99%

Electroporation as a vaccine delivery system and a natural adjuvant to intradermal administration of plasmid DNA in macaques

Todorova

Adam

Čulina

et al. 2017

Sci Rep

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“…Synthetic nanomaterial-based delivery systems can be beneficial for these DNA-based therapeutics [126, 127]. Similar to DNA delivery in other biological transfection processes, DNA encoding antigens can also be delivered by various synthetic and natural nanosystems, including PLGA [128, 129], PEI/PGA [130], chitosan [131, 132], cationic lipids [133, 134], grapheme and carbon nanotubes[135], and even hybrid particles [136, 137]. …”

Section: Application Of Nanomaterials For Cancer Vaccine Designmentioning

confidence: 99%

Nanomaterials for cancer immunotherapy

2017

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Cancer immunotherapy is quickly growing to be the fourth most important cancer therapy, after surgery, radiation therapy, and chemotherapy. Immunotherapy is the most promising cancer management strategy because it orchestrates the body’s own immune system to target and eradicate cancer cells, which may result in durable antitumor responses and reduce metastasis and recurrence more than traditional treatments. Nanomaterials hold great promise in further improving the efficiency of cancer immunotherapy - in many cases, they are even necessary for effective delivery. In this review, we briefly summarize the basic principles of cancer immunotherapy and explain why and where to apply nanomaterials in cancer immunotherapy, with special emphasis on cancer vaccines and tumor microenvironment modulation.

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“…These mini-DNA systems, as well as the selection of a proper concentration, and perhaps volume of DNA may yield a high level of gene expression via electroporation. The volume and DNA concentration are mentioned here because results suggest that electroporation interacts with these parameters to enhance the gene transfer efficiency and the level of gene expression(17). …”

Section: Effects Of Dna Properties On Dna Electroporationmentioning

confidence: 99%

The Impact of Non-electrical Factors on Electrical Gene Transfer

Cutrera

2014

Methods in Molecular Biology

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Electrical pulses directly and effectively boost both in vitro and in vivo gene transfer, but this process is greatly affected by non-electrical factors that exist during electroporation. These factors include, but are not limited to, the types of cells or tissues used, the property of DNA, DNA formulation, and the expressed protein. In this mini-review, we only describe and discuss a summary of DNA properties and selected DNA formulations on gene transfer via electroporation. The properties of DNA were selected for review because a substantial amount of remarkable work has been performed during the past few years but has received less notice than other work, although DNA properties appear to be critical for boosting electroporation delivery. The selected formulations will be covered in this mini-review because we are only interested in the simple formulations that could be used for cell or gene therapy via electroporation. Plus, there was an extensive review of DNA formulations in the first edition of this book. The formulations discussed in this mini-review represent novel developments in recent years and may impact electroporation significantly. These advancements in DNA formulations could prove to be important for gene delivery and disease treatment.

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In vivo electroporation enhances the potency of poly-lactide co-glycolide (PLG) plasmid DNA immunization

Cited by 15 publications

References 43 publications

Electroporation as a vaccine delivery system and a natural adjuvant to intradermal administration of plasmid DNA in macaques

Electroporation as a vaccine delivery system and a natural adjuvant to intradermal administration of plasmid DNA in macaques

Nanomaterials for cancer immunotherapy

The Impact of Non-electrical Factors on Electrical Gene Transfer

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