In-vivo electrochemical monitoring of H2O2 production induced by root-inoculated endophytic bacteria in Agave tequilana leaves

Lima, Alex Silva; Prieto, Kátia R.; Santos, Carla Santana; Valerio, Hellen Paula; García-Ochoa, Evelyn Y.; Huerta-Robles, Aurora; Beltrán-García, Miguel J.; Mascio, Paolo Di; Bertotti, Mauro

doi:10.1016/j.bios.2017.07.039

Cited by 38 publications

(22 citation statements)

References 53 publications

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“…For a long time there has been concern about plant beneficial endophytes because they are closely related to human and animal opportunistic and pathogenic microbes (Rosenblueth et al, 2004; Mishra et al, 2017; Lima et al, 2018). Based on analyses of in vitro hemolysis of red blood cells and antibiotic resistance, we show that K. pneumoniae causes β-hemolysis and may be considered virulent, but E. cloacae has no hemolytic activity and is not likely virulent (Supplementary Figure S1).…”

Section: Discussionmentioning

confidence: 99%

Enterobacter cloacae, an Endophyte That Establishes a Nutrient-Transfer Symbiosis With Banana Plants and Protects Against the Black Sigatoka Pathogen

et al. 2019

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Banana ( Musa spp.) is an important crop worldwide, but black Sigatoka disease caused by the fungus Pseudocercospora fijiensis threatens fruit production. In this work, we examined the potential of the endophytes of banana plants Enterobacter cloacae and Klebsiella pneumoniae , as antagonists of P. fijiensis and support plant growth in nutrient limited soils by N-transfer. The two bacterial isolates were identified by MALDI-TOF mass spectrometry and corroborated by 16S rRNA sequence analysis. Both bacteria were positive for beneficial traits such as N-fixation, indole acetic acid production, phosphate solubilization, negative for 1-aminocyclopropane 1-carboxylic acid deaminase and were antagonistic to P. fijiensis . To measure the effects on plant growth, the two plant bacteria and an E. coli strain (as non-endophyte), were inoculated weekly for 60 days as active cells (AC) and heat-killed cells (HKC) into plant microcosms without nutrients and compared to a water only treatment, and a mineral nutrients solution (MMN) treatment. Bacterial treatments increased growth parameters and prevented accelerated senescence, which was observed for water and mineral nutrients solution (MMN) treatments used as controls. Plants died after the first 20 days of being irrigated with water; irrigation with MMN enabled plants to develop some new leaves, but plants lost weight (−30%) during the same period. Plants treated with bacteria showed good growth, but E. cloacae AC treated plants had significantly greater biomass than the E. cloacae HKC. After 60 days, plants inoculated with E. cloacae AC showed intracellular bacteria within root cells, suggesting that a stable symbiosis was established. To evaluate the transference of organic N from bacteria into the plants, the 3 bacteria were grown with 15 NH 4 Cl or Na 15 NO 3 as the nitrogen source. The 15 N transferred from bacteria to plant tissues was measured by pheophytin isotopomer abundance. The relative abundance of the isotopomers m/z 872.57, 873.57, 874.57, 875.57, 876.57 unequivocally demonstrated that plants acquired 15 N atoms directly from bacterial cells, using them as a source of N, to support plant growth in restricted nutrient soils. E. cloacae might be a new alternative to promote growth and health of banana crops.

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Section: Discussionmentioning

confidence: 99%

Enterobacter cloacae, an Endophyte That Establishes a Nutrient-Transfer Symbiosis With Banana Plants and Protects Against the Black Sigatoka Pathogen

et al. 2019

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“…H 2 O 2 production will reach ≈6000 kt in 2024, worth US$6.4 billion . It is necessary to real‐time monitor H 2 O 2 during its production because residues of H 2 O 2 is harmful to the environment . However, traditional methods such as spectrophotometry are tedious and inconducive to realize online monitoring of H 2 O 2 .…”

Section: Introductionmentioning

confidence: 99%

Cobalt Nanoparticles and Atomic Sites in Nitrogen‐Doped Carbon Frameworks for Highly Sensitive Sensing of Hydrogen Peroxide

Liu

Tang

et al. 2019

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In situ monitoring of hydrogen peroxide (H2O2) during its production process is needed. Here, an electrochemical H2O2 sensor with a wide linear current response range (concentration: 5 × 10−8 to 5 × 10−2 m), a low detection limit (32.4 × 10−9 m), and a high sensitivity (568.47 µA mm−1 cm−2) is developed. The electrocatalyst of the sensor consists of cobalt nanoparticles and atomic Co‐Nx moieties anchored on nitrogen doped carbon nanotube arrays (Co‐N/CNT), which is obtained through the pyrolysis of the sandwich‐like urea@ZIF‐67 complex. More cobalt nanoparticles and atomic Co‐Nx as active sites are exposed during pyrolysis, contributing to higher electrocatalytic activity. Moreover, a portable screen‐printed electrode sensor is constructed and demonstrated for rapidly detecting (cost ≈40 s) H2O2 produced in microbial fuel cells with only 50 µL solution. Both the synthesis strategy and sensor design can be applied to other energy and environmental fields.

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“…For the striking release of H 2 O 2 in agave, tequilana leaves are correlated with a number of plant-pathogen interactions or environmental stresses and may play an important role in disease resistance. 34 The samples were diluted 100 times with a PBS solution (pH 7.0) and measured by the standard addition method. As listed in Table 1, the recovery for the determination of the H 2 O 2 sample was 96.1%-103.7% and the RSD was less than 3.8%.…”

Section: Application In Real Samplesmentioning

confidence: 99%

A novel enzymatic biosensor for detection of intracellular hydrogen peroxide based on 1-aminopyrene and reduced graphene oxides

et al. 2019

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An electrochemical sensor for hydrogen peroxide (H 2 O 2) detection was established using 1aminopyrene (AP) as a linker between horseradish peroxidase (HRP) and reduced graphene oxides (rGO) modified glassy carbon electrode. The AP can combine with HRP via a covalent bond and combine with rGO via π-π conjugation. The covalent bond linkages can prevent the enzymes leaching of the enzymes and the π-π conjugate combination can facilitate electrons transfer, which plays synergistic effects to improve the performance of this sensor. The electrochemical activity of the HRP-AP/rGO was described by cyclic voltammetry, alternating current impedance and amperometric techniques. Under the optimal conditions, the biosensor showed a wide linear range from 1.5 μM to 28.5 μM and a low detection limit of 0.5 μM with good stability and high selectivity, confirming that the sensor is well-suited for the detection of H 2 O 2 during biological processes.

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In-vivo electrochemical monitoring of H2O2 production induced by root-inoculated endophytic bacteria in Agave tequilana leaves

Cited by 38 publications

References 53 publications

Enterobacter cloacae, an Endophyte That Establishes a Nutrient-Transfer Symbiosis With Banana Plants and Protects Against the Black Sigatoka Pathogen

Enterobacter cloacae, an Endophyte That Establishes a Nutrient-Transfer Symbiosis With Banana Plants and Protects Against the Black Sigatoka Pathogen

Cobalt Nanoparticles and Atomic Sites in Nitrogen‐Doped Carbon Frameworks for Highly Sensitive Sensing of Hydrogen Peroxide

A novel enzymatic biosensor for detection of intracellular hydrogen peroxide based on 1-aminopyrene and reduced graphene oxides

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