In Vivo Electrical Stimulation for the Assessment of Skeletal Muscle Contractile Function in Murine Models

Vitzel, Kaio Fernando; Fortes, Marco Aurélio Salomão; Marzuca‐Nassr, Gabriel Nasri; Scervino, Maria V. M.; Pinheiro, Carlos Hermano da Justa; Silveira, Leonardo R.; Curi, Rui

doi:10.1007/978-1-4939-7614-0_26

Cited by 7 publications

(5 citation statements)

References 22 publications

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“…In the test presented, the tetanic curve starts at ~60 Hz, where the potentiation can be visualized (Figure 4A) and the maximum force is determined at ~150 Hz (Figure 4B), when the plateau is reached with a completed fused curve 9,16 . Any variation of these results may indicate that the muscles are not being properly stimulated by the electrodes.…”

Section: Representative Resultsmentioning

confidence: 99%

“…The force-frequency curve is a useful test in which muscles can be stimulated by lower and higher frequencies to distinguish suboptimal and optimal force responses 15 . The force at lower frequencies can stimulate a single twitch, activating fewer and smaller motor units, and at higher frequencies a stable peak is reached, where isolated twitches fused (tetanus), reaching maximum force through activating all motor units 16 .…”

Section: Representative Resultsmentioning

confidence: 99%

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Non-invasive Assessment of Dorsiflexor Muscle Function in Mice

Gerlinger-Romero

Addinsall

Lovering

et al. 2019

JoVE

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Assessment of skeletal muscle contractile function is an important measurement for both clinical and research purposes. Numerous conditions can negatively affect skeletal muscle. This can result in a loss of muscle mass (atrophy) and/or loss of muscle quality (reduced force per unit of muscle mass), both of which are prevalent in chronic disease, muscle-specific disease, immobilization, and aging (sarcopenia). Skeletal muscle function in animals can be evaluated by a range of different tests. All tests have limitations related to the physiological testing environment, and the selection of a specific test often depends on the nature of the experiments. Here, we describe an in vivo, non-invasive technique involving a helpful and easy assessment of force frequency-curve (FFC) in mice that can be performed on the same animal over time. This permits monitoring of disease progression and/or efficacy of a potential therapeutic treatment.

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Section: Representative Resultsmentioning

confidence: 99%

Section: Representative Resultsmentioning

confidence: 99%

Non-invasive Assessment of Dorsiflexor Muscle Function in Mice

Gerlinger-Romero

Addinsall

Lovering

et al. 2019

JoVE

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“…The method of electrical stimulation was modified from previous studies 7,37 . The muscles of the lower extremity were simultaneously stimulated on both sides using electrical stimulation at 7-8 Hz with a pulse width of 250 μs, eliciting a continuous twitch for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Low-frequency electrical stimulation of bilateral hind legs by belt electrodes is effective for preventing denervation-induced atrophies in multiple skeletal muscle groups in rats

Uno

Kamiya²,

Akimoto³

et al. 2022

Sci Rep

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Belt electrode skeletal muscle electrical stimulation (B-SES) can simultaneously contract multiple muscle groups. Although the beneficial effects of B-SES in clinical situations have been elucidated, its molecular mechanism remains unknown. In this study, we developed a novel rodent B-SES ankle stimulation system to test whether low-frequency stimulation prevents denervation-induced muscle atrophy. Electrical stimulations (7‒8 Hz, 30 min) with ankle belt electrodes were applied to Sprague–Dawley rats daily for one week. All animals were assigned to the control (CONT), denervation-induced atrophy (DEN), and DEN + electrical stimulation (ES) groups. The tibialis anterior (TA) and gastrocnemius (GAS) muscles were used to examine the effect of ES treatment. After seven daily sessions of continuous stimulation, muscle wet weight (n = 8–11), and muscle fiber cross-sectional area (CSA, n = 4–6) of TA and GAS muscles were lower in DEN and DEN + ES than in CON. However, it was significantly higher in DEN than DEN + ES, showing that ES partially prevented muscle atrophy. PGC-1α, COX-IV, and citrate synthase activities (n = 6) were significantly higher in DEN + ES than in DEN. The mRNA levels of muscle proteolytic molecules, Atrogin-1 and Murf1, were significantly higher in DEN than in CONT, while B-SES significantly suppressed their expression (p < 0.05). In conclusion, low-frequency electrical stimulation of the bilateral ankles using belt electrodes (but not the pad electrodes) is effective in preventing denervation-induced atrophy in multiple muscles, which has not been observed with pad electrodes. Maintaining the mitochondrial quantity and enzyme activity by low-frequency electrical stimulation is key to suppressing muscle protein degradation.

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“…The force at lower frequencies can stimulate a single twitch, activating fewer and smaller motor units, and at higher frequencies a stable peak is reached, where isolated twitches fused (tetanus), reaching maximum force through activating all motor units 16 . In the test presented, the tetanic curve starts at ~60 Hz, where the potentiation can be visualized (Figure 4A) and the maximum force is determined at ~150 Hz (Figure 4B), when the plateau is reached with a completed fused curve 9,16 . Any variation of these results may indicate that the muscles are not being properly stimulated by the electrodes.…”

Section: Representative Resultsmentioning

confidence: 99%