In vivo electrical impedance measurement in human skin assessment

Kubisz, Leszek; Hojan‐Jezierska, Dorota; Szewczyk, Maria T.; Majewska, Anna; Kawałkiewicz, Weronika; Pankowski, Edward; Januś, M.; Cwajda‐Białasik, Justyna; Mościcka, Paulina; Jawień, Arkadiusz

doi:10.1515/pac-2018-1106

Cited by 12 publications

(21 citation statements)

References 26 publications

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“…It is less sensitive to the hydration status of the skin and can easily measure small changes in electrical resistance of hydrated skin, including at sites where micropores have been created [2]. Many studies have reported significant differences in skin impedance measured at various anatomical locations [24,25] and attributed these observations to differences in skin thickness, hydration level, and activity of sweat glands [25]. The results in our study showed that baseline skin impedance at the abdomen was significantly higher than at the upper arm across all subjects, and within the Latino and black self-identified racial/ethnic groups.…”

Section: Baseline Epidermal Propertiesmentioning

confidence: 99%

Micropore Closure Rates following Microneedle Application at Various Anatomical Sites in Healthy Human Subjects

Ogunjimi

Lawson

Carr

et al. 2021

Skin Pharmacol Physiol

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Introduction: The continuous availability of open micropores is crucial for a successful microneedle (MN) drug delivery strategy. However, micropore lifetime depends on intrinsic skin functional and anatomical characteristics, which vary significantly at different anatomical sites. Objective: This pilot study explored if differences exist in micropore closure timeframes at 3 anatomical sites – upper arm, volar forearm, and abdomen. Methods: Healthy subjects (n = 35) self-identifying as Asian (n = 9), Bi-/multiracial (n = 2), Black (n = 9), Latino (n = 6), and White (n = 9) completed the study. The upper arm, volar forearm, and abdomen were treated with MNs; skin impedance and transepidermal water loss (TEWL) were measured at baseline and post-MN to confirm micropore formation. Impedance was measured for 3 days to evaluate micropore lifetime. Measurements of L*, which quantifies the skin lightness/darkness, were made using a tristimulus colorimeter. Micropore lifetime was determined by comparing baseline and post-MN impedance measurements, and micropore closure half-life was predicted using mathematical modeling. Results: Post-MN increase in TEWL and decrease in impedance were significant (p < 0.05), confirming successful micropore formation at all anatomical sites. When data were analyzed according to subject self-identified racial/ethnic groups, the mean micropore closure time at the abdomen (63.09 ± 13.13 h) was longer than the upper arm (60.34 ± 14.69 h) and volar forearm (58.29 ± 16.76 h). The predicted micropore closure half-life at anatomical sites was the abdomen (25.86 ± 14.96 h) ≈ upper arm (23.69 ± 13.67 h) > volar forearm (20.2 ± 11.99 h). Differences were not statistically significant between groups. Objective categorization by L* showed that the darker skin may be associated with longer micropore closure time at the abdomen site. Conclusions: Our results suggest that anatomical site of application may not be a source of significant variability in micropore closure time. These findings may help reduce the number of physiological parameters that need to be explicitly considered when developing drug products to support MN-assisted drug delivery strategies.

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Section: Baseline Epidermal Propertiesmentioning

confidence: 99%

Micropore Closure Rates following Microneedle Application at Various Anatomical Sites in Healthy Human Subjects

Ogunjimi

Lawson

Carr

et al. 2021

Skin Pharmacol Physiol

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“…It is often stressed that some of the collagen production methods may be expensive and cost-ineffective, which results in the limited use of collagen biomateri-als in the treatment of chronic wounds [13]. In our study we used collagen from the skin of freshwater fish (silver carp), which is inexpensive, easily available and characterized by good physicochemical properties [13,24,33,34,49]. Fish collagen has a relatively low molecular weight (less intermolecular crosslinking), which makes it possible to obtain a fully biologically active molecule in the form of helix.…”

Section: Discussionmentioning

confidence: 99%

“…In the preliminary analysis conducted in the group of 59 patients with VLUs we assessed the effect of the formulation on periwound skin and its ability to penetrate the corneal layer of the epidermis [49]. Electrical impedance measurements showed the improvement in the overall skin condition [49] while cutometry showed the improvement in skin elasticity and firmness after the use of collagen. In the placebo group, skin improvement was not significant [49,51,52].…”

Section: Discussionmentioning

confidence: 99%

“…Electrical impedance measurements showed the improvement in the overall skin condition [49] while cutometry showed the improvement in skin elasticity and firmness after the use of collagen. In the placebo group, skin improvement was not significant [49,51,52]. It is worth noting that the positive effect of collagen on the skin was noticeable only from week 8 .…”

Section: Discussionmentioning

confidence: 99%

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Venous leg ulcers treated with fish collagen gel in a 12-week randomized single-centre study

Cwajda‐Białasik¹,

Mościcka²,

Szewczyk³

et al. 2022

pdia

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Introduction: Venous ulcers are difficult to heal chronic wounds. Most challenging are the non-healing and recurrent ulcers, because of which we are seeking for therapies that stimulate and support their healing. Aim: To assess the efficacy of fish collagen and its impact on the process of healing of venous leg ulcers. Material and methods: This 12-week randomized single-centre study included a total of 97 adults with venous leg ulcer, randomized into two groups: the study group (n = 48) and the control group (n = 49). All patients received specialist medical care including skin and limb care, wound development according to the TIME scheme (tissue debridement, infection and inflammation control, moisture balance, edge) and short-stretch bandage compression (compression class II). In addition, the study group received tropocollagen gel applied to the skin around the wound. Every 2 weeks we evaluated the wound surface and temperature distribution on the shin (average temperature, median, minimum and maximum) -symmetrically on the sick and healthy limb. Results: We observed a greater number of complete ulcer healing in the study group (week 12: 29.2% vs. 22.4%; week 24: 52.1% vs. 36.7%). Faster healing time in the study group was observed in both big and small ulcers. The thermographic analysis showed statistically significant reduction in periwound inflammation among patients from the study group (p < 0.05). Statistically significant reduction in mean as well as maximum values of limb temperature occurred only in the study group (Tx: R = 0.37, p < 0.001; Tmax: R = 0.40, p < 0.001). Conclusions: Thermographic analysis showed better wound healing effects in the group using tropocollagen. Significant impact of collagen on the healing process was observed only after 8 weeks of application.

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“…The uncovering of collagen fibers in damaged tissues triggers a chain of cellular and biochemical reactions initiating the process of blood clotting and healing of tissues. This property of collagen is utilized in the process of homeostasis initiated by means of a collagen sponge applied on the wound [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Effects of Temperature on the FT NIR Raman Spectra of Fish Skin Collagen

et al. 2021

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The development of regenerative medicine turns attention toward native collagen as a biocompatible material. Particularly interesting is fish skin collagen, which is relatively easy to extract comparing mammalian tissues and free of some pathogens that are dangerous to humans. The paper presents results of IR Raman spectroscopy studies of silver carp (Hypophthalmichthys molitrix) skin collagen. As collagen properties result from its structure and conformation, both sensitive to temperature, FT NIR Raman spectroscopy is an excellent tool to characterize the molecular structure of fish skin collagen, particularly in temperature range typical for the manufacturing processes of biomedical products. Therefore, the Raman spectra were recorded in a temperature range of 300 to 403 K. The analysis of Raman spectra of prepared collagen films, particularly in the range of the bands related to amide I and amide III entities, showed a high content of α-helix and α-helix type molecular organization in fish skin collagen. Additionally, the secondary structure of the studied fish skin collagen is stable up to ~358 K. Heating to 403 K leads to irreversible changes in the molecular structure of fish skin collagen. It was found that the Raman spectrum of fish skin collagen preheated in this manner becomes similar to the spectrum of the collagen obtained from bovine Achilles tendon, whose secondary structure does not change up to 403 K.

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In vivo electrical impedance measurement in human skin assessment

Cited by 12 publications

References 26 publications

Micropore Closure Rates following Microneedle Application at Various Anatomical Sites in Healthy Human Subjects

Micropore Closure Rates following Microneedle Application at Various Anatomical Sites in Healthy Human Subjects

Venous leg ulcers treated with fish collagen gel in a 12-week randomized single-centre study

Effects of Temperature on the FT NIR Raman Spectra of Fish Skin Collagen

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