In vivo Efficacy of Biophytum petersianum on Haemonchus contortus in Goats

Abstract

H aemonchosis is one of the major parasitic diseases in goats in Indonesia. The biggest economic losses due to haemonchosis occur in subtropical and tropical regions which are mainly due to mortality, decreased production, stunted growth and low body weight (Mengist et al., 2014). In fact, West Papua could be a potential development area for goat livestock. Based on data from the Department of Animal Husbandry of West Papua Province (2016), the research Article Abstract | Haemonchosis is a common and severe disease caused by the infection of worms Haemonchus sp. Kebar grass (Biophytum petersianum) known as a plant from Papua Indonesia that contains tannin compounds and has potential anthelmintic activity. The present study was carried out to determine the influence of Kebar grass infusion on H. contortus under in vivo conditions. A total of 15 female goats at 6-8 months old were divided into 5 groups, and each consisted of 3 goats. All groups were infected 1000 infective larvae orally every week for 4 weeks. Three groups were given Kebar grass infusion a day at a dose of 2 mg/mL, 4 mg/mL and 6 mg/mL respectively for 7 days at the 6th week. Group 4 was given Albendazole at a dose of 3.8 mg/kg body weight, and group 5 was not given treatment. Clinical examinations, hematological and EPG were carried out every week. All goats were autopsied and analyzed for pathology at the seventh week. Quantitative data to perform statistical analysis, as well as the results of physical examination and histopathology were analyzed descriptively. Body weight measurements in vivo as the mean, Body Condition Scores (BCS), and FAMACHA showed an increase. RBC, Hb, PCV and total protein values increased. The FECR value of the treatment group was higher than the negative control group. During the autopsy, both carcass and visceral organs were colourless, slight of subcutaneous fat, and small intestine haemorrhage and ascites in the abdominal cavity. Many nodules with diameters between 1.5-2.0 mm were observed in the abomasal mucosa. Histopathological changes in the control group were infiltration of inflammatory cells, congestion and the presence of worm pieces. Based on results obtained in this line of research, it is therefore concluded that Kebar grass infusion is anthelmintic against H. contortus under in vivo conditions.

Anthelmintic effect of Indigofera tinctoria L on Haemonchus contortus obtained from sheep in Indonesia

“…González-Cortazar et al [36] stated that the aqueous extract of L. acapulcensis leaves reduced the EPG value of gastrointestinal nematodes in sheep. Sambodo et al [14] also stated that the aqueous extract of Biophytum petersianum decreased the EPG value of H. contortus in goats. The decrease in the EPG value is believed to be caused by secondary compounds in I. tinctoria.…”

“…A number of I. tinctoria L. leaves were cleaned, dried in a drying room, and stored until they were used for the extraction process. Each of the dry I. tinctoria leaves (10,12,14,16,18,20, and 22 g) was placed in 100 mL of distilled water and then incubated at 90°C for 15 min. The solution was filtered using filter paper, and the filtrate (100, 120, 140, 160, 180, 200, and 220 mg/mL) was stored in the refrigerator until SEM and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were conducted [10].…”

“…The variables analyzed included body weight, EPG, hematology results, and SEM. Observations, data collection, and testing were carried out on days 0, 7, and 14 [14]. SEM was performed using worms found in the abomasum of slaughtered sheep.…”

“…Blood was collected from all sheep through the jugular vein (2 mL/sheep). The parameters analyzed included hemoglobin (Hb), packed cell volume (PCV), and total erythrocyte count (TEC) [14].…”

The ultrastructure changes of Haemonchus contortus exposed to bamboo leaves (Gigantochloa apus) aqueous extract under in vitro condition

“…Min et al (2005) have proven that increased protein consumption will decrease parasite infections by increasing host immunity. Sambodo et al (2020) also report that condensed tannin concentrations over 55 g per kg dry feed ingredients will decrease feed intake, decrease digestibility, and suppress average daily weight gain of goats.…”