In Vivo Effects of Single Intra-Articular Injection of 0.5% Bupivacaine on Articular Cartilage

Abstract

This in vivo study showing reduced chondrocyte density without cartilage tissue loss six months after a single intra-articular injection of 0.5% bupivacaine suggests bupivacaine toxicity. The effects of bupivacaine were milder than those of an injection of 0.6% monoiodoacetate, which resulted in chondrolysis over the same time period.

Persistence, Localization, and External Control of Transgene Expression After Single Injection of Adeno-Associated Virus into Injured Joints

“…However, we have extensive experience in accurate intra-articular (IA) injection of AAV and other substrates in rat knee joints (Chu et al, 2010;Payne et al, 2011). In addition, we imaged the preinjury AAV-injected joints with IA luciferin injection at a weekly interval following surgery and found no difference in signal patterns from preinjury time points.…”

“…The bioluminescence signal pattern was no different from those imaged and quantified using the IA luciferin administration route the week prior (28-day postinjury or the 1-month timepoint). As previously mentioned, IA injections into rat stifle joints have been extensively and reliably utilized in our previous studies (Chu et al, 2010;Payne et al, 2011).…”

Articular cartilage and local anaesthetic: A systematic review of the current literature

“…In their final study, Chu et al 23 used an in vivo technique, injecting rat knee joints with 100 ml 0.9% saline control or 0.5% bupivacaine. They then analysed the cartilage at one week, four weeks, 12 weeks and six months using confocal live/dead cell microscopy, histological analysis and quantitative cell density analysis.…”

The Efficacy of Intra-Articular Injections for Pain Control Following the Closed Reduction and Percutaneous Pinning of Pediatric Supracondylar Humeral Fractures

“…In contrast, a pig and dog knee model revealed no significant differences in histological findings and/or cartilage anabolism between saline solution-treated and bupivacaine-treated samples three days after injection 49 . In a rat knee model, there was no difference in superficial chondrocyte viability between joint samples exposed to a single injection of 0.50% bupivacaine compared with saline solution up to six months after exposure 50 . We are not aware of any reported cases of chondrolysis of the elbow joint associated with the use of intra-articular injections of local anesthetics in the current literature, despite the frequency of their use during elbow arthroscopy.…”