In vivo effects of propolis, a honeybee product, on gilthead seabream innate immune responses

Cuesta, Alberto; Rodrı́guez, Alejandro; Meseguer, José

doi:10.1016/j.fsi.2004.06.002

Cited by 116 publications

(89 citation statements)

References 28 publications

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“…These fibres were found in other fish and mammalians (Acosta et al 2005;Schubert-Schoppmeyer et al 2008) and they were correlated with poor meat quality. Finally, as previously described, the SGR was not significantly affected by the dietary intake of propolis at 10 g propolis·kg -1 diet according to the results of Cuesta et al (2005) and in discordance with Abd-El-Rhman (2009) according to who propolisethanolic -extract and crude propolis evoked the highest specific growth rate.…”

Section: Body Length and Fibre Typesupporting

confidence: 79%

“…Several authors have demonstrated the biological benefits of propolis but it is hard to compare the present findings and the current results with the literature because reports on the addition of propolis to diets deal with its effects on the immune response in fish (Cuesta 2005;Chu 2006). Talas (2009) reported that propolis has protective haematological and biochemical effects on rainbow trout.…”

Section: Body Length and Fibre Typementioning

confidence: 56%

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Effect of Propolis on the Fish Muscular Development and Histomorphometrical Characteristics

Velotto¹,

Vitale²,

Varricchio

et al. 2010

Acta Vet. Brno

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The aim of our study was to evaluate the effect of propolis on mortality of fish eggs due to mycosis, to study its efficacy on the fish growth rate and to analyze the histochemical and ultrastructural characteristics of muscle fibres. We evaluated the muscular development of fish in two different experimental conditions: traditional feeding and feeding with addition of propolis (groups A and B, respectively). The study was carried out on two species of teleosts bred in Italy, Oncorhynchus mykiss (rainbow trout) and Salmo trutta (brown trout). The use of propolis reduced mortality of fish eggs. The muscles were stained for myosin ATPase and succinic dehydrogenase to evaluate fibre type. The area, maximum and minimum diameter for each fibre type in relation to the body length were measured. The animals fed propolis showed a more rapid muscular growth compared to control fed the standard diet. Fibres were larger in the Salmo trutta than in the Oncorhynchus mykiss in both groups. In fish of A group, myotomal muscle, the presence of giant fibres and index of environmental stress was noticed. This type of study is valuable in order to extend the use of propolis as an antifungal agent and a natural product that can improve fish farming. ATPase and SDH activities, specific growth-rate, ultrastructural analysisEuropean aquaculture has shown a steady increase during the last twenty years, thanks to many initiatives promoted by the European Community to support this sector. But there are still many problems to solve, such as the support of ecocompatible activities, the improvement of animal health and welfare, product quality and consumer health assurance. Propolis is a mixture of various amounts of beeswax and resins collected by the honeybee from plants, particularly from flowers and leaf buds (Krell 1996). The colour of propolis ranges from yellow to dark brown depending on the origin of the resins. Propolis has attracted the researchers' interest in the last decades due to its biological and pharmacological properties, such as immunomodulatory, antitumor, antimicrobial, antiinflammatory, and antioxidant properties (Kanbur et al. 2009). The goal of this study was to analyze, for the first time, the influence of propolis on the fish muscular development and on the structural and ultrastructural characteristics of muscle fibre types. This study opens a new perspective on the investigation of biological properties of propolis, mainly with respect to the influence on the development of the trout and the mortality of fish eggs. Materials and MethodsThe following species of freshwater fish were used in our study: Salmo trutta (brown trout) and Oncorhynchus mykiss (rainbow trout). Experiment with eggsIn order to evaluate the effect of propolis on mortality of fish eggs, approximately 600 eggs (half and half) were placed in 3 "Californian tanks" arranged in cascade. Untreated samples (about 250 eggs) were used as control. The eggs were rinsed in propolis solution (1%) for 15 min, once every four days and then they were ...

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Section: Body Length and Fibre Typesupporting

confidence: 79%

Section: Body Length and Fibre Typementioning

confidence: 56%

Effect of Propolis on the Fish Muscular Development and Histomorphometrical Characteristics

Velotto¹,

Vitale²,

Varricchio

et al. 2010

Acta Vet. Brno

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show abstract

“…Similarly, Cuesta et al (2005) have related increased number of monocyte-macrophages and granulocytes phagocytosis from the blood of Sparus aurata fed supplemented diet with propolis. On the other hand, a gradated increase in leucocyte accumulation on the inflammatory site induced by Escherichia coli injection was reported for tilapia with the highest migration activity 24 hours after injection (Matsuyama and Iida, 1999).…”

Section: Discussionmentioning

confidence: 91%

Acute inflammation and hematological response in Nile tilapia fed supplemented diet with natural extracts of propolis and Aloe barbadensis

et al. 2015

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This study evaluated the acute inflammatory response induced by carrageenin in the swim bladder of Nile tilapia supplemented with the mixture of natural extracts of propolis and Aloe barbadensis (1:1) at a concentration of 0.5%, 1% and 2% in diet during 15 days. Thirty-six fish were distributed into four treatments with three replicates: fish supplemented with 0.5% of admix of extracts of propolis and Aloe (1:1) injected with 500 µg carrageenin; fish supplemented with 1% of admix of extracts of propolis and Aloe (1:1) injected with 500 µg carrageenin; fish supplemented with 2% of admix of extracts of propolis and Aloe (1:1), injected with 500 µg carrageenin and unsupplemented fish injected with 500 µg carrageenin. Six hours after injection, samples of blood and exudate from the swim bladder of fish were collected. It was observed an increase in the leukocyte count in the swim bladder exudate of fish supplemented with extracts of propolis and Aloe injected with carrageenin. The most frequent cells were macrophages followed by granular leukocytes, thrombocytes and lymphocytes. Supplementation with propolis and Aloe to 0.5% caused a significant increase in the number of cells on the inflammatory focus mainly macrophages, cells responsible for the phagocytic activity in tissues, agent of innate fish immune response.Keywords: inflammation, carrageenin, swim bladder, propolis, Aloe.Inflamação aguda e resposta hematológica em tilápias do Nilo alimentadas com extratos naturais de própolis e Aloe barbadensis suplementados na dieta ResumoEste estudo avaliou a resposta inflamatória aguda induzida por carragenina na bexiga natatóriade tilápia do Nilo suplementada com a mistura dos extratos naturais de própolis e Aloe barbadensis (1:1), nas concentrações de 0,5%, 1% e 2% na dieta durante o período de 15 dias. Trinta e seis peixes foram distribuídos em quatro tratamentos com três repetições: peixes suplementados com 0,5% da mistura dos extratos de própolis e Aloe (1:1) injetados na bexiga natatória com 500 µg de carragenina; peixes suplementados com 1% da mistura dos extratos de própolis e Aloe (1:1) injetados na bexiga natatória com 500 µg de carragenina; peixes suplementados com 2% da mistura dos extratos de própolis e Aloe (1:1) injetados na bexiga natatória com 500 µg de carragenina e peixes não suplementados injetados na bexiga natatória com 500 µg de carragenina. Seis horas após as injeções foram coletadas amostras de sangue e exsudato da bexiga natatória dos peixes. Foi observado aumento na contagem de leucócitos no exsudato da bexiga natatória de peixes suplementados com os extratos de própolis e Aloe injetados com carragenina. As células mais frequentes foram os macrófagos seguidos pelos leucócitos granulares, trombócitos e linfócitos. A suplementação com própolis e Aloe a 0,5% provocou aumento significativo no número de células no foco inflamatório, principalmente dos macrófagos, células responsáveis pela atividade fagocitária nos tecidos, agente da resposta imune inata nos peixes.Palavras-chave: inflamação,...

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“…This has allowed us to get a lot of information about the regulation of the seabream immune response, and concretely the NCC activity. Thus, we have shown in vitro and/or in vivo modulation of seabream NCC activity by vitamins C (Cuesta et al, 2002b), E , A (Cuesta et al, 2003b) and D3 (Cerezuela et al, 2009), chitin (Cuesta et al, 2003c;Esteban et al, 2000Esteban et al, , 2001, levamisole (Cuesta et al, 2002c), lactoferrin , melatonin (Cuesta et al, 2008a), propolis (Cuesta et al, 2005b), inulin (Cerezuela et al, 2008), unmethylated oligodeoxynucleotides (ODNs) containing cytosine-phosphodiester-guanosine (CpG) motifs (Cuesta et al, 2008b(Cuesta et al, , 2008c, probiotic bacteria Salinas et al, 2005Salinas et al, , 2006Salinas et al, , 2008, yeast (Cuesta et al, 2007;Ortuño et al, 2002;Reyes-Becerril et al, 2008;Rodríguez et al, 2003), fungi (Rodríguez et al, 2004), virus , environmental contaminants (p,p´-DDE and lindane) (Cuesta et al, 2008d) or stress factors (air exposure, crowding and anaesthetics) (Cuesta et al, 2003d). In general, we have demonstrated great NCC increments after these treatments.…”

Section: Modulation Of the Fish Cytotoxic Activitymentioning

confidence: 86%

Fighting Virus and Parasites with Fish Cytotoxic Cells

Esteban¹,

Meseguer²,

Cuesta³

2012

Health and Environment in Aquaculture

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In vivo effects of propolis, a honeybee product, on gilthead seabream innate immune responses

Cited by 116 publications

References 28 publications

Effect of Propolis on the Fish Muscular Development and Histomorphometrical Characteristics

Effect of Propolis on the Fish Muscular Development and Histomorphometrical Characteristics

Acute inflammation and hematological response in Nile tilapia fed supplemented diet with natural extracts of propolis and Aloe barbadensis

Fighting Virus and Parasites with Fish Cytotoxic Cells

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