In vivo effects of fluconazole on lymphocyte subpopulations of the thymus and spleen in mice: Flow cytometry analysis

Liu, J.; Mengel, Klaus; Friedberg, K. D.

doi:10.1016/s0192-0561(96)00049-5

Cited by 6 publications

(7 citation statements)

References 9 publications

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“…The procedure for flow cytometry (FCM) staining followed the method described by Liu [ 14 ]. Briefly, the cells of each sample were stained with anti-CD4 RPE and anti-CD3 FITC, or anti-CD8 Alexa Flour 647 and anti-CD3 FITC.…”

Section: Methodsmentioning

confidence: 99%

Comparison of worm development and host immune responses in natural hosts of schistosoma japonicum, yellow cattle and water buffalo

Yang

Feng

et al. 2012

BMC Vet Res

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BackgroundYellow cattle and water buffalo are two of the most important natural hosts for Schistosoma japonicum in China. Previous observation has revealed that yellow cattle are more suited to the development of S. japonicum than water buffalo. Understanding more about the molecular mechanisms involved in worm development, as well as the pathological and immunological differences between yellow cattle and water buffalo post infection with S japonicum will provide useful information for the vaccine design and its delivery procedure.ResultsThe worm length (p < 0.01), worm recovery rate (p < 0.01) and the percentage of paired worms (p < 0.01) were significantly greater in yellow cattle than those in water buffalo. There were many white egg granulomas in the livers of yellow cattle, but fewer were observed in water buffalo at 7 weeks post infection. The livers of infected yellow cattle contained significantly increased accumulation of inflammatory cells, and the schistosome eggs were surrounded with large amounts of eosinophil infiltration. In contrast, no hepatocyte swelling or lymphocyte infiltration, and fewer white blood cells, was observed in water buffalo. The percentage of CD4+ T cells was higher in yellow cattle, while the percentage of CD8+ T cells was higher in water buffalo from pre-infection to 7 w post infection. The CD4/CD8 ratios were decreased in both species after challenge with schistosomes. Comparing with water buffalo, the IFN-γ level was higher and decreased significantly, while the IL-4 level was lower and increased gradually in yellow cattle from pre-infection to 7 w post infection.ConclusionsIn this study, we confirmed that yellow cattle were more suited to the development of S. japonicum than water buffalo, and more serious pathological damage was observed in infected yellow cattle. Immunological analysis suggested that CD4+ T cells might be an integral component of the immune response and might associate with worm development in yellow cattle. A shift from Th1 to Th2 type polarized immunity was only shown clearly in schistosome-infected yellow cattle, but no shift in water buffalo. The results provide valuable information for increased understanding of host-schistosome interactions, and for control of schistosomiasis.

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Section: Methodsmentioning

confidence: 99%

Comparison of worm development and host immune responses in natural hosts of schistosoma japonicum, yellow cattle and water buffalo

Yang

Feng

et al. 2012

BMC Vet Res

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“…Vital cells were counted by means of trypan blue dye exclusion staining and adjusted to 5×10 6 cells/mL in fluorescence-activated cell sorter (FACS) buffer (PBS, 1% FCS, 0.1% sodium azide, 5% normal mouse serum). The procedure for flow cytometry (FCM) staining has been described [32] . Briefly, the rats and M. fortis splenocytes were stained with fluorescein (FITC)-conjugated anti-rat CD4 and phycoerythrin (PE)-conjugated anti-rat CD8 and mouse splenocytes were stained with FITC-conjugated anti-mouse CD4 and PE-conjugated anti-mouse CD8.…”

Section: Methodsmentioning

confidence: 99%

Study on Differences in the Pathology, T Cell Subsets and Gene Expression in Susceptible and Non-Susceptible Hosts Infected with Schistosoma japonicum

et al. 2010

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More than 40 kinds of mammals in China are known to be naturally infected with Schistosoma japonicum (S. japonicum); Microtus fortis (M. fortis), a species of vole, is the only mammal in which the schistosomes cannot mature or cause significant pathogenic changes. In the current study, we compared the differences in pathology by Hematoxylin-eosin staining and in changes in the T cell subsets with flow cytometry as well as gene expression using genome oligonucleotide microarrays in the lung and liver, before challenge and 10 days post-infection with schistosomes in a S. japonicum-susceptible mouse model of infection, a non-susceptible rat model and the non-permissive host, M. fortis. The results demonstrated that S. japonicum promoted a more intensive immune response and more pathological lesions in M. fortis and rats than in mice. Hematoxylin-eosin staining revealed that the immune effector cells involved were mainly eosinophilic granulocytes supplemented with heterophilic granulocytes and macrophages. The analysis of splenic T cell subsets showed that CD4+ T cell subsets and the CD4+/CD8+ ratio were increased, while the CD8+ T cell subsets decreased remarkably in rats; whereas the CD8+ T cell subsets were increased, but the CD4+/CD8+ ratio was decreased significantly in mice. The analysis of the pattern of gene expression suggested that some immune-associated genes and apoptosis-inducing genes up-regulated, while some development-associated genes were down-regulated in the infected M. fortis compared to the uninfected controls; the three different hosts have different response mechanisms to schistosome infection. The results of this study will be helpful for identifying the key molecules in the immune response to S. japonicum in M. fortis and for understanding more about the underlying mechanism of the response, as well as for elucidating the interaction between S. japonicum and its hosts.

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“…In contrast, the organ-to-body weight ratios of treatment with compound 6 were not significantly different from that of NS. These results suggest that compound 6 did not affect body weights and the organ-to-body weight ratio of mice at the treatment dose, which suggests it was not toxic at that dose …”

Section: Results and Discussionmentioning

confidence: 99%

Design and Synthesis of Biotinylated Bivalent Carboline Derivatives as Potent Antitumor Agents

et al. 2020

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Compound 6, a novel β-carboline comprising two 1-methyl-9H-βcarboline-3-carboxylic acids and a biotin moiety conjugated together using tris(2aminoethyl)amine, was synthesized and tested for its cytotoxicity toward MCF-7 and HepG2 cell lines and antitumor potency in an S180 tumor-bearing mouse model. Compound 6 was delivered via biotin receptor-mediated endocytosis and exerted its therapeutic effects by intercalation binding with DNA. In vivo antitumor evaluations of 6 revealed that it is efficacious and exhibits low systemic toxicity.

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In vivo effects of fluconazole on lymphocyte subpopulations of the thymus and spleen in mice: Flow cytometry analysis

Cited by 6 publications

References 9 publications

Comparison of worm development and host immune responses in natural hosts of schistosoma japonicum, yellow cattle and water buffalo

Comparison of worm development and host immune responses in natural hosts of schistosoma japonicum, yellow cattle and water buffalo

Study on Differences in the Pathology, T Cell Subsets and Gene Expression in Susceptible and Non-Susceptible Hosts Infected with Schistosoma japonicum

Design and Synthesis of Biotinylated Bivalent Carboline Derivatives as Potent Antitumor Agents

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