In vivo effects of a thymosin α1-containing colostral whey product on neutrophils and lymphocytes from lactating cows without and with experimentally induced Staphylococcus aureus mastitis

Abstract

Two separate experiments evaluated ID-1 (a commercial bovine whey product containing 5200 pg of thymosin α 1 /ml) as an immunotherapeutic agent in lactating cows. In the first experiment, cows without mastitis were evaluated for blood leukogram, milk production, total and differential milk cell counts, lymphocyte (Lc) blastogenesis, and neutrophil (PMN) functions (random and directed migration under agarose, chemiluminescence, ingestion of bacteria, iodination, cytochrome C reduction, antibodyindependent neutrophil-mediated cytotoxicity, and antibody-dependent cell-mediated cytotoxicity) before and after ID-1 therapy. ID-1 treatment resulted in a significant treatment group by time period interaction for the relative proportion of mononuclear cells (MNC) in milk (P<0.009) and for PMN random migration (P<0.01). Based on these interactions, ID-1 treatment appeared to slightly increase the proportion of small MNC in milk and to increase random migration from pretreatment levels by 73% more than increases observed in controls. No significant effect of ID-1 treatment on milk production, total milk somatic cell counts, Lc blastogenesis, or other PMN functions was observed. In cows with experimental Staphylococcus aureus intramammary infections, ID-1 treatment resulted in a significant decline in blood leukocyte count (P<0.001) and blood PMN count (P<0.02), and maintained PMN random migration (P<0.01) while controls declined and abrogated a depression in the ability of Lc to respond to mitogens (P<0.05) that developed in controls as a result of S. aureus mastitis. Injection of ID-1 into cows had no adverse effect on their overall health or level of milk production, but did cause subtle and potentially favorable changes in several in vitro immune parameters. In spite of these subtle changes which might indicate increased resistance to mastitis, cows actually developed a more severe S. aureus intramammary infection based on a 9% increase in log 10 bacterial shedding in milk. Veterinary Immunology and Immunopathology, 20 ( 1989) Two separate experiments evaluated ID-1 (a commercial bovine whey product containing 5200 pg ofthymosin a 1 /ml) as an immunotherapeutic agent in lactating cows. In the first experiment, cows without mastitis were evaluated for blood leukogram, milk production, total and differential milk cell counts, lymphocyte (Lc) blastogenesis, and neutrophil (PMN) functions (random and directed migration under agarose, chemiluminescence, ingestion of bacteria, iodination, cytochrome C reduction, antibody-independent neutrophil-mediated cytotoxicity, and antibody-dependent cell-mediated cytotoxicity) before and after ID-1 therapy. ID-1 treatment resulted in a significant treatment group by time period interaction for the relative proportion of mononuclear cells (MNC) in milk (P < 0.009) and for PMN random migration (P < 0.01). Based on these interactions, ID-1 treatment appeared to slightly increase the proportion of small MNC in milk and to increase random migration from pretreatment levels by 73% m...

Management of mastitis on organic and conventional dairy farms1

“…Ultrafiltered bovine whey has been shown to have the ability to enhance in vitro neutrophil activity (Kehrli et al, 1989;Roth et al, 2001). The ability of these products to successfully treat subclinical or clinical mastitis has not been described.…”

Pegbovigrastim Treatment around Parturition Enhances Postpartum Immune Response Gene Network Expression of whole Blood Leukocytes in Holstein and Simmental Cows

“…The negative energy balance of dairy cows during the transition period is associated with reduced immune function and increased concentrations of some blood metabolites due to tissue mobilization [1,2]. Polymorphonuclear neutrophil (PMN) and lymphocyte functions decrease gradually starting about 2 wk before calving, with the lowest efficiencies between time of calving and two days after [3,4].…”

Απομονωση Και Φυσικοχημικος Χαρακτηρισμος Των Α- Και Β-Θυμοσινων Της Πεστροφας (Salmo Gairdneri)

“…γ) Σε καλλιέργειες λεμφοκυττάρων, χορήγηση θυμοσίνης α-j αυξάνει την σύνθεση αντισωμάτων έναντι της γρίππης, κυρίως σε μεγαλύτερης ηλικίας άτομα που από μόνα τους ενεφάνιζαν μικρότερη ικανότητα παραγωγής αντισωμάτων (Ershler et al, 1984). δ) Βοηθά στην θεραπεία της πειραματικά προκαλούμενης από Staphylococcus aureus μαστίτιδας αγελάδων (Kehrli et al, 1989).…”