In vivo determination of 5-bromo-2′-deoxyuridine incorporation into DNA tumor tissue by a new 32P-postlabelling thin-layer chromatographic method

Nucleic Acids and Their Derivatives

“…Exposure times enhance the ability to label unknowns at high counts in as little as 2 h, but typically run 24-72 h. Present densitometric techniques can range from a few minutes for one-dimensional analyses to 2 h for complete analyses of a 20 X 20 cm autoradiogram. Comparisons of techniques to direct scintillation counting approach r values of 0.99 and similarly correlate with the best quantifying techniques (7)(8)(9)12,50,55).…”

“…Intermediate-chain oligonucleotides are nicely handled by HPLC, but many smaller ones are not. Silica gel TLC has been important in oligomer separations well up to decamers (50)(51)(52)(53).…”

“…It is extremely easy to compare prior TLC plates, generate tables for comparison, and rapidly apply statistical or analytical methods by computer. This has yielded quantifiable relationships in nucleic acid and DNA chemistry that were difficult to examine previously (7)(8)(9)12,50,55).…”

“…The better ability to subtract controls, carry out statistical analyses, and enhance minute adducts magnifies adduct and analog detection of nucleic acids. Some studies have presented data on phosphoroimager use and TLC including "high power" focusing carried out by computer interaction (7)(8)(9)12,50,55).…”

“…Thin-layer chromatography has been used in the study of metabolic diseases; illicit drug use; toxicology, including mycotoxins; environmental injury; and, particularly, analytical drug characterization and quantification for both diagnostic and regulatory purposes (7)(8)(9)12,50,55,62,63). It has extensive applications for the pharmaceutical industry in all areas of drug development: synthesis, separation, drug screening, batch quality testing, and government monitoring and reg- ulations.…”